AFF2 (NM_001169123) Human Tagged ORF Clone

CAT#: RG230695

AFF2 (GFP-tagged) - Human AF4/FMR2 family, member 2 (AFF2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AFF2
• Synonyms: FMR2; FMR2P; FRAXE; MRX2; OX19
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230695 representing NM_001169123
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTATTCGACTTTTTCAGAGACTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTG
  CACTTAAAAAAAGGGAATGGGAGCGGAGGAATCAAGAAGTCCAGCAAGAAGACGATCTCTTTTCTTCAGG
  CTTTGATCTTTTTGGGGAGCCATACAAGACAAACAAAGGTGATGCACTTGCCAACCGAGTCCAGAACACG
  CTTGGAAACTATGATGAAATGAAGAATTTGCTAACTAACCATTCTAATCAGAATCACCTAGTGGGAATTC
  CAAAGAATTCTGTGCCCCAGAATCCCAACAACAAAAATGAACCAAGCTTTTTTCCAGAACAAAAGAACAG
  AATAATTCCACCTCACCAGGATAATACCCATCCTTCAGCACCAATGCCTCCACCTTCTGTTGTGATACTG
  AATTCAACTCTAATACACAGCAACAGAAAATCAAAACCTGAGTGGTCACGTGATAGTCATAACCCTAGCA
  CTGTACTGGCAAGCCAGGCCAGTGGTCAGCCAAACAAGATGCAGACTTTGACACAGGACCAGTCTCAAGC
  CAAACTGGAAGACTTCTTTGTCTACCCAGCTGAACAGCCCCAGATTGGAGAAGTTGAAGAGTCAAACCCA
  TCTGCAAAGGAAGACAGTAACCCTAATTCTAGTGGAGAAGATGCTTTCAAAGAAATCTTTCAATCCAATT
  CACCGGAAGAATCTGAATTCGCCGTGCAAGCGCCTGGGTCTCCCCTAGTGGCTTCCTCTTTATTAGCTCC
  TAGCAGTGGCCTTTCAGTTCAAAACTTCCCACCAGGGCTTTACTGCAAAACAAGCATGGGGCAGCAAAAG
  CCAACTGCATACGTCAGACCCATGGATGGCCAGGACCAGGCACCGGACATCTCACCAACACTGAAACCTT
  CAATTGAATTTGAGAACAGCTTTGGGAATCTGTCATTTGGAACACTCTTGGATGGAAAACCCAGTGCAGC
  CAGTTCAAAGACTAAACTGCCAAAGTTCACCATCCTCCAAACAAGTGAAGTAAGCCTTCCCAGTGATCCA
  AGCTGTGTTGAAGAAATCTTGCGGGAGATGACCCATTCCTGGCCTACTCCTCTCACTTCCATGCATACTG
  CTGGACACTCTGAGCAGAGCACCTTTTCCATCCCAGGACAGGAATCGCAGCATCTGACCCCAGGATTCAC
  CTTACAAAAGTGGAATGACCCAACCACCAGAGCTTCTACAAAGATGCTTGAGGATGACCTGAAGCTGAGC
  AGTGATGAAGATGACCTTGAGCCTGTGAAGACCTTGACCACTCAGTGCACTGCCACTGAGCTCTACCAGG
  CTGTTGAAAAGGCAAAACCTAGGAATAATCCTGTGAACCCACCCTTGGCCACTCCCCAGCCCCCACCTGC
  AGTGCAAGCCAGCGGGGGTTCTGGCAGCTCCAGCGAATCGGAGAGCAGCTCTGAGTCGGATTCAGACACT
  GAAAGTAGCACCACTGACAGCGAATCTAATGAGGCACCTCGTGTGGCAACTCCAGAGCCTGAGCCACCCT
  CAACCAACAAGTGGCAACTGGATAAATGGCTTAACAAAGTGACATCCCAGAACAAGTCTTTTATTTGTGG
  CCAAAATGAAACACCCATGGAGACTATTTCTCTGCCTCCTCCAATCATCCAACCAATGGAAGTCCAGATG
  AAAGTGAAGACGAATGCCAGTCAGGTCCCAGCTGAACCCAAAGAAAGGCCTCTCCTCAGTCTCATTAGGG
  AGAAAGCCCGTCCACGGCCCACTCAGAAAATTCCAGAAACAAAGGCTTTGAAGCATAAGTTGTCAACAAC
  TAGTGAGACAGTGTCTCAAAGGACAATTGGGAAAAAACAGCCCAAAAAAGTTGAGAAGAACACCAGCACT
  GACGAGTTTACCTGGCCCAAACCAAATATTACCAGCAGCACTCCCAAAGAAAAAGAAAGTGTGGAGCTTC
  ATGACCCACCAAGAGGCCGCAACAAAGCCACTGCCCACAAACCAGCCCCTAGGAAAGAACCAAGACCTAA
  CATCCCTTTGGCTCCCGAGAAGAAGAAGTACAGAGGGCCTGGCAAGATTGTGCCAAAGTCTCGGGAATTC
  ATTGAAACAGATTCATCTACATCTGACTCCAACACAGATCAGGAAGAGACCCTGCAAATCAAAGTCCTGC
  CTCCGTGCATTATTTCTGGAGGTAATACTGCCAAATCCAAGGAAATCTGTGGTGCCAGCCTGACCCTCAG
  CACCTTAATGAGTAGCAGTGGCAGCAACAACAACTTATCCATCAGTAATGAAGAGCCAACATTTTCACCT
  ATTCCTGTCATGCAAACTGAAATCCTGTCCCCTCTGCGAGATCATGAGAACCTGAAAAACCTCTGGGTGA
  AGATTGACCTTGACTTACTCTCTAGAGTACCTGGCCACAGCTCACTCCATGCAGCACCTGCCAAGCCAGA
  CCACAAGGAGACTGCCACAAAACCCAAGCGTCAGACAGCTGTCACAGCTGTGGAGAAACCAGCCCCTAAG
  GGCAAACGTAAGCACAAGCCAATAGAAGTTGCAGAGAAGATCCCTGAGAAGAAGCAGCGCCTGGAGGAGG
  CCACAACTATCTGCTTGCTCCCTCCTTGCATCTCACCAGCCCCACCCCACAAGCCTCCCAACACTAGAGA
  AAATAATTCATCCAGGAGAGCAAATAGAAGAAAGGAAGAAAAACTATTTCCTCCTCCACTTTCCCCACTG
  CCAGAGGACCCTCCACGCCGCAGAAATGTCAGTGGCAATAATGGTCCCTTTGGTCAAGACAAAAACATCG
  CCATGACTGGACAAATCACATCTACCAAACCTAAGAGAACTGAAGGCAAATTCTGTGCTACTTTCAAAGG
  GATATCGGTAAATGAGGGAGACACTCCAAAAAAGGCATCCTCTGCCACCATCACTGTCACCAATACTGCT
  ATTGCCACTGCTACTGTCACTGCTACTGCCATTGTCACCACCACTGTCACAGCTACTGCCACCGCCACGG
  CCACCACCACAACTACTACCACTACCATTTCCACCATCACCTCTACCATCACTACTGGCCTCATGGATAG
  CAGTCACCTGGAGATGACGTCCTGGGCGGCTCTGCCCCTTCTATCCAGCAGCAGCACTAATGTCCGGAGA
  CCCAAGCTCACTTTTGATGACTCGGTTCACAATGCTGATTATTACATGCAAGAAGCTAAGAAGCTGAAGC
  ACAAAGCTGATGCACTGTTCGAGAAATTTGGCAAAGCTGTGAATTATGCTGATGCCGCCCTCTCCTTCAC
  TGAATGTGGCAATGCCATGGAACGCGACCCTCTGGAAGCAAAGTCCCCATACACCATGTACTCTGAGACT
  GTGGAGCTCCTCAGGTATGCAATGAGGCTGAAGAACTTTGCAAGTCCCTTGGCTTCGGATGGGGACAAAA
  AGCTAGCAGTACTATGCTACCGATGTTTATCACTCCTCTATTTGAGAATGTTTAAGCTGAAGAAGGACCA
  TGCTATGAAGTACTCCAGATCACTGATGGAATATTTTAAGCAAAATGCTTCAAAAGTCGCACAGATACCC
  TCTCCATGGGTAAGCAATGGAAAGAACACTCCATCCCCAGTGTCTCTCAACAACGTCTCCCCCATCAACG
  CAATGGGGAACTGTAACAATGGCCCAGTCACCATTCCCCAGCGCATTCACCACATGGCTGCCAGCCACGT
  CAACATCACTAGCAATGTGTTACGGGGCTATGAACACTGGGATATGGCCGACAAACTGACAAGAGAAAAC
  AAAGAATTCTTTGGTGATCTGGACACGCTGATGGGGCCTCTGACCCAGCACAGCAGCATGACCAATCTTG
  TCCGCTACGTTCGCCAAGGACTGTGTTGGCTGCGCATCGATGCCCACTTGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230695 representing NM_001169123
  Red=Cloning site Green=Tags(s)
  
  MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLFSSGFDLFGEPYKTNKGDALANRVQNT
  LGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVIL
  NSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNP
  SAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSMGQQK
  PTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLPKFTILQTSEVSLPSDP
  SCVEEILREMTHSWPTPLTSMHTAGHSEQSTFSIPGQESQHLTPGFTLQKWNDPTTRASTKMLEDDLKLS
  SDEDDLEPVKTLTTQCTATELYQAVEKAKPRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSDT
  ESSTTDSESNEAPRVATPEPEPPSTNKWQLDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPMEVQM
  KVKTNASQVPAEPKERPLLSLIREKARPRPTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTST
  DEFTWPKPNITSSTPKEKESVELHDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREF
  IETDSSTSDSNTDQEETLQIKVLPPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFSP
  IPVMQTEILSPLRDHENLKNLWVKIDLDLLSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAPK
  GKRKHKPIEVAEKIPEKKQRLEEATTICLLPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFPPPLSPL
  PEDPPRRRNVSGNNGPFGQDKNIAMTGQITSTKPKRTEGKFCATFKGISVNEGDTPKKASSATITVTNTA
  IATATVTATAIVTTTVTATATATATTTTTTTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVRR
  PKLTFDDSVHNADYYMQEAKKLKHKADALFEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSET
  VELLRYAMRLKNFASPLASDGDKKLAVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQIP
  SPWVSNGKNTPSPVSLNNVSPINAMGNCNNGPVTIPQRIHHMAASHVNITSNVLRGYEHWDMADKLTREN
  KEFFGDLDTLMGPLTQHSSMTNLVRYVRQGLCWLRIDAHLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001169123
• ORF Size: 3903 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001169123.1, NP_001162594.1
• RefSeq Size: 13716 bp
• RefSeq ORF: 3906 bp
• Locus ID: 2334
• Cytogenetics: Xq28
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016]'