PRODH (NM_001195226) Human Tagged ORF Clone

CAT#: RG230843

PRODH (GFP-tagged) - Human proline dehydrogenase (oxidase) 1 (PRODH), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRODH
• Synonyms: HSPOX2; PIG6; POX; PRODH1; PRODH2; TP53I6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230843 representing NM_001195226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGACCTTCTATGGGCATTTTGTAGCCGGGGAGGACCAGGAGTCCATCCAGCCCCTGCTTCGGC
  ACTACAGGGCCTTCGGTGTCAGCGCCATCCTGGACTATGGAGTGGAGGAGGACCTGAGCCCCGAGGAGGC
  AGAGCACAAGGAGATGGAGTCCTGCACCTCAGCTGCGGAGAGGGATGGCAGTGGCACGAATAAGCGGGAC
  AAGCAATACCAGGCCCACCGGGCCTTCGGGGACCGCAGGAATGGTGTCATCAGTGCCCGCACCTACTTCT
  ACGCCAATGAGGCCAAGTGCGACAGCCACATGGAGACATTCTTGCGCTGCATCGAAGCCTCAGGTAGAGT
  CAGCGATGACGGCTTCATAGCCATTAAGCTCACAGCACTGGGGAGACCCCAGTTTCTGCTGCAGTTCTCA
  GAGGTGCTGGCCAAGTGGAGGTGCTTCTTTCACCAAATGGCTGTGGAGCAAGGGCAGGCGGGCCTGGCTG
  CCATGGACACCAAGCTGGAGGTGGCGGTGCTGCAGGAAAGTGTCGCAAAGTTGGGCATCGCATCCAGGGC
  TGAGATTGAGGACTGGTTCACGGCAGAGACCCTGGGAGTGTCTGGCACCATGGACCTGCTGGACTGGAGC
  AGCCTCATCGACAGCAGGACCAAGCTGTCCAAGCACCTGGTAGTCCCCAACGCACAGACAGGACAGCTGG
  AGCCCCTGCTGTCCCGGTTCACTGAGGAGGAGGAGCTACAGATGACCAGGATGCTACAGCGGATGGATGT
  CCTGGCCAAGAAAGCCACAGAGATGGGCGTGCGGCTGATGGTGGATGCCGAGCAGACCTACTTCCAGCCG
  GCCATCAGCCGCCTGACGCTGGAGATGCAGCGGAAGTTCAATGTGGAGAAGCCGCTCATCTTCAACACAT
  ACCAGTGCTACCTCAAGGATGCCTATGACAATGTGACCCTGGACGTGGAGCTGGCTCGCCGTGAGGGCTG
  GTGTTTTGGGGCCAAGCTGGTGCGGGGCGCATACCTGGCCCAGGAGCGAGCCCGTGCGGCAGAGATCGGC
  TATGAGGACCCCATCAACCCCACGTACGAGGCCACCAACGCCATGTACCACAGGTGCCTGGACTACGTGT
  TGGAGGAGCTGAAGCACAACGCCAAGGCCAAGGTGATGGTGGCCTCCCACAATGAGGACACAGTGCGCTT
  TGCACTGCGCAGGATGGAGGAGCTGGGCCTGCATCCTGCTGACCACCGGGTGTACTTTGGACAGCTGCTA
  GGCATGTGTGACCAGATCAGCTTCCCGCTGGGCCAGGCTGGCTACCCCGTGTACAAGTACGTGCCCTATG
  GCCCCGTGATGGAGGTGCTGCCCTACTTGTCCCGCCGTGCCCTGGAGAACAGCAGCCTCATGAAGGGCAC
  CCATCGGGAGCGGCAGCTGCTGTGGCTGGAGCTCTTGAGGCGGCTCCGAACTGGCAACCTCTTCCATCGC
  CCTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230843 representing NM_001195226
  Red=Cloning site Green=Tags(s)
  
  MKMTFYGHFVAGEDQESIQPLLRHYRAFGVSAILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRD
  KQYQAHRAFGDRRNGVISARTYFYANEAKCDSHMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFS
  EVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVAVLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWS
  SLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTEEEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQP
  AISRLTLEMQRKFNVEKPLIFNTYQCYLKDAYDNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIG
  YEDPINPTYEATNAMYHRCLDYVLEELKHNAKAKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLL
  GMCDQISFPLGQAGYPVYKYVPYGPVMEVLPYLSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHR
  PA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195226
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195226.1, NP_001182155.1
• RefSeq Size: 2008 bp
• RefSeq ORF: 1479 bp
• Locus ID: 5625
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]'