Dystrobrevin alpha (DTNA) (NM_001198944) Human Tagged ORF Clone

CAT#: RG231206

DTNA (GFP-tagged) - Human dystrobrevin, alpha (DTNA), transcript variant 16

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DTNA
• Synonyms: D18S892E; DRP3; DTN; DTN-A; LVNC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231206 representing NM_001198944
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCAGATCAGCCTGAGAAGCCACTCAACTTGGCTCACATCGTGCCTCCCAGACCTGTAACCAGCA
  TGAACGACACCCTGTTCTCCCACTCTGTTCCCTCCTCAGGAAGTCCTTTTATTACCAGGAGGTTACCTGA
  GGGAATAAGTGCATCCAGCCCTGTGGCTGAAGAGCATTCCCTCATAAAGCTGTACGTAAATCAGCTTGAT
  CACGGTGCACGCATGCTTGAGAGTTCAAACCGGCTTGATGAAGAACACAGGCTAATTGCCAGGTATGCGG
  CAAGGCTGGCAGCAGAGTCCTCTTCGTCTCAGCCACCTCAGCAGAGAAGTGCTCCTGACATCTCTTTCAC
  CATCGATGCGAATAAGCAGCAAAGGCAGCTGATTGCTGAGCTAGAAAACAAGAACAGAGAAATCTTACAG
  GAGATCCAGAGACTTCGGCTAGAGCATGAACAAGCTTCTCAGCCCACGCCAGAGAAGGCACAGCAAAACC
  CCACCCTGCTGGCAGAACTCCGGCTCCTCAGACAGCGCAAAGATGAGCTGGAACAGAGAATGTCTGCTCT
  CCAGGAGAGCCGGAGAGAGCTAATGGTCCAGTTGGAGGGTCTCATGAAGCTACTAAAGACTCAGGGGGCA
  GGCTCTCCCCGCTCCTCCCCCAGCCACACCATCAGCAGGCCAATTCCCATGCCCATCCGGTCAGCGTCAG
  CCTGCTCCACCCCGACGCACACGCCGCAGGACTCCCTCACAGGAGTAGGGGGAGATGTACAAGAGGCATT
  TGCACAAAGTTCAAGAAGAAACTTAAGGAATGACTTGCTAGTGGCTGCAGATTCCATCACTAACACTATG
  TCCTCTCTTGTGAAAGAGCTGAATTCTGAGGTTGGGAGTGAAACAGAGAGTAATGTGGATTCTGAATTTG
  CACGGACTCAGTTTGAGGATCTTGTTCCCTCACCAACCTCTGAAAAGGCTTTTCTAGCGCAAATCCATGC
  CCGAAAACCTGGGTACATTCACAGTGGAGCTACCACAAGTACCATGCGTGGCGACATGGTTACGGAGGAT
  GCAGATCCCTATGTGCAGCCTGAAGATGAAAACTATGAAAATGACTCTGTCCGGCAGCTGGAGAATGAGC
  TCCAGATGGAGGAATACCTGAAACAGAAGCTGCAAGATGAAGCTTATCAGGTCAGCTTGCAAGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231206 representing NM_001198944
  Red=Cloning site Green=Tags(s)
  
  MFPDQPEKPLNLAHIVPPRPVTSMNDTLFSHSVPSSGSPFITRRLPEGISASSPVAEEHSLIKLYVNQLD
  HGARMLESSNRLDEEHRLIARYAARLAAESSSSQPPQQRSAPDISFTIDANKQQRQLIAELENKNREILQ
  EIQRLRLEHEQASQPTPEKAQQNPTLLAELRLLRQRKDELEQRMSALQESRRELMVQLEGLMKLLKTQGA
  GSPRSSPSHTISRPIPMPIRSASACSTPTHTPQDSLTGVGGDVQEAFAQSSRRNLRNDLLVAADSITNTM
  SSLVKELNSEVGSETESNVDSEFARTQFEDLVPSPTSEKAFLAQIHARKPGYIHSGATTSTMRGDMVTED
  ADPYVQPEDENYENDSVRQLENELQMEEYLKQKLQDEAYQVSLQG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198944
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198944.1, NP_001185873.1
• RefSeq Size: 5741 bp
• RefSeq ORF: 1188 bp
• Locus ID: 1837
• Cytogenetics: 18q12.1
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene belongs to the dystrobrevin subfamily of the dystrophin family. This protein is a component of the dystrophin-associated protein complex (DPC), which consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and alpha- and beta-dystrobrevin. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Mutations in this gene are associated with left ventricular noncompaction with congenital heart defects. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]'