Oct-1 (POU2F1) (NM_001198786) Human Tagged ORF Clone

CAT#: RG231382

POU2F1 (GFP-tagged) - Human POU class 2 homeobox 1 (POU2F1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POU2F1
• Synonyms: oct-1B; OCT1; OTF1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231382 representing NM_001198786
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGGAGGAGCAGCGAGTCAAGATGAGAGTTCAGCCGCGGCGGCAGCAGCAGCAGACTCAAGAA
  TGAACAATCCGTCAGAAACCAGTAAACCATCTATGGAGAGTGGAGATGGCAACACAGGCACACAAACCAA
  TGGTCTGGACTTTCAGAAGCAGCCTGTGCCTGTAGGAGGAGCAATCTCAACAGCCCAGGCGCAGGCTTTC
  CTTGGACATCTCCATCAGGTCCAACTCGCTGGAACAAGTTTACAGGCTGCTGCTCAGTCTTTAAATGTAC
  AGTCTAAATCTAATGAAGAATCGGGGGATTCGCAGCAGCCAAGCCAGCCTTCCCAGCAGCCTTCAGTGCA
  GGCAGCCATTCCCCAGACCCAGCTTATGCTAGCTGGAGGACAGATAACTGGGGATCTTCAACAACTGCAA
  CAGCTTCAACAGCAGAATCTCAACCTGCAACAGTTTGTGTTGGTGCATCCAACCACCAATTTGCAGCCAG
  CGCAGTTTATCATCTCACAGACGCCCCAGGGCCAGCAGGGTCTCCTGCAAGCGCAAAATCTTCTAACGCA
  ACTACCTCAGCAAAGCCAAGCCAACCTCCTACAGTCGCAGCCAAGCATCACCCTCACCTCCCAGCCAGCA
  ACCCCAACACGCACAATAGCAGCAACCCCAATTCAGACACTTCCACAGAGCCAGTCAACACCAAAGCGAA
  TTGATACTCCCAGCTTGGAGGAGCCCAGTGACCTTGAGGAGCTTGAGCAGTTTGCCAAGACCTTCAAACA
  AAGACGAATCAAACTTGGATTCACTCAGGGTGATGTTGGGCTCGCTATGGGGAAACTATATGGAAATGAC
  TTCAGCCAAACTACCATCTCTCGATTTGAAGCCTTGAACCTCAGCTTTAAGAACATGTGCAAGTTGAAGC
  CACTTTTAGAGAAGTGGCTAAATGATGCAGAGAACCTCTCATCTGATTCGTCCCTCTCCAGCCCAAGTGC
  CCTGAATTCTCCAGGAATTGAGGGCTTGAGCCGTAGGAGGAAGAAACGCACCAGCATAGAGACCAACATC
  CGTGTGGCCTTAGAGAAGAGTTTCTTGGAGAATCAAAAGCCTACCTCGGAAGAGATCACTATGATTGCTG
  ATCAGCTCAATATGGAAAAAGAGGTGATTCGTGTTTGGTTCTGTAACCGCCGCCAGAAAGAAAAAAGAAT
  CAACCCACCAAGCAGTGGTGGGACCAGCAGCTCACCTATTAAAGCAATTTTCCCCAGCCCAACTTCACTG
  GTGGCGACCACACCAAGCCTTGTGACTAGCAGTGCAGCAACTACCCTCACAGTCAGCCCTGTCCTCCCTC
  TGACCAGTGCTGCTGTGACGAATCTTTCAGTTACAGGCACTTCAGACACCACCTCCAACAACACAGCAAC
  CGTGATTTCCACAGCGCCTCCAGCTTCCTCAGCAGTCACGTCCCCCTCTCTGAGTCCCTCCCCTTCTGCC
  TCAGCCTCCACCTCCGAGGCATCCAGTGCCAGTGAGACCAGCACAACACAGACCACCTCCACTCCTTTGT
  CCTCCCCTCTTGGGACCAGCCAGGTGATGGTGACAGCATCAGGTTTGCAAACAGCAGCAGCTGCTGCCCT
  TCAAGGAGCTGCACAGTTGCCAGCAAATGCCAGTCTTGCTGCCATGGCAGCTGCTGCAGGACTAAACCCA
  AGCCTGATGGCACCCTCACAGTTTGCGGCTGGAGGTGCCTTACTCAGTCTGAATCCAGGGACCCTGAGCG
  GTGCTCTCAGCCCAGCTCTAATGAGCAACAGTACACTGGCAACTATTCAAGCTCTTGCTTCTGGTGGCTC
  TCTTCCAATAACATCACTTGATGCAACTGGGAACCTGGTATTTGCCAATGCGGGAGGAGCCCCCAACATC
  GTGACTGCCCCTCTGTTCCTGAACCCTCAGAACCTCTCTCTGCTCACCAGCAACCCTGTTAGCTTGGTCT
  CTGCCGCCGCAGCATCTGCAGGGAACTCTGCACCTGTAGCCAGCCTTCACGCCACCTCCACCTCTGCTGA
  GTCCATCCAGAACTCTCTCTTCACAGTGGCCTCTGCCAGCGGGGCTGCGTCCACCACCACCACCGCCTCC
  AAGGCACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231382 representing NM_001198786
  Red=Cloning site Green=Tags(s)
  
  MADGGAASQDESSAAAAAAADSRMNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAF
  LGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGDLQQLQ
  QLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPA
  TPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGND
  FSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNI
  RVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSL
  VATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSA
  SASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNP
  SLMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNI
  VTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTAS
  KAQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198786
• ORF Size: 2109 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198786.1, NP_001185715.1
• RefSeq Size: 13716 bp
• RefSeq ORF: 2112 bp
• Locus ID: 5451
• Cytogenetics: 1q24.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]'