PDE1C (NM_001191057) Human Tagged ORF Clone

CAT#: RG231386

PDE1C (GFP-tagged) - Human phosphodiesterase 1C, calmodulin-dependent 70kDa (PDE1C), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDE1C
• Synonyms: cam-PDE 1C; DFNA74; hCam-3; Hcam3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231386 representing NM_001191057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGCCAACCAAGGAGATTGAAGAATTTGAGAGCAACTCTCTGAAATACCTGCAACCGGAACAGA
  TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGATTACGGTCTTT
  GGTCAAACAATTAGAGAGAGGGGAAGCTTCAGTGGTAGATCTTAAGAAGAATTTGGAATATGCAGCCACA
  GTGCTTGAATCTGTGTATATTGATGAAACAAGGAGACTCCTGGATACAGAGGATGAGCTCAGTGACATTC
  AGTCAGATGCTGTGCCTTCTGAGGTCCGAGACTGGCTGGCCTCCACCTTCACGCGGCAGATGGGGATGAT
  GCTCAGGAGGAGCGACGAGAAGCCCCGGTTCAAGAGCATCGTTCACGCAGTGCAGGCTGGGATATTTGTG
  GAGAGAATGTATAGACGGACATCAAACATGGTTGGACTGAGCTATCCACCAGCTGTTATTGAGGCATTAA
  AGGATGTGGACAAGTGGTCCTTTGACGTCTTTTCCCTCAATGAGGCCAGTGGGGATCATGCACTGAAATT
  TATTTTCTATGAACTACTCACACGTTATGATCTGATCAGCCGTTTCAAGATCCCCATTTCTGCACTTGTC
  TCATTTGTGGAGGCCCTGGAAGTGGGATACAGCAAGCACAAAAATCCTTACCATAACTTAATGCACGCTG
  CCGATGTTACACAGACAGTGCATTACCTCCTCTATAAGACAGGAGTGGCGAACTGGCTGACGGAGCTGGA
  GATCTTTGCTATAATCTTCTCAGCTGCCATCCATGACTACGAGCATACCGGAACCACCAACAATTTCCAC
  ATTCAGACTCGGTCTGATCCAGCTATTCTGTATAATGACAGATCTGTACTGGAGAATCACCATTTAAGTG
  CAGCTTATCGCCTTCTGCAAGATGACGAGGAAATGAATATTTTGATTAACCTCTCAAAGGATGACTGGAG
  GGAGTTTCGAACCTTGGTAATTGAAATGGTGATGGCCACAGATATGTCTTGTCACTTCCAACAAATCAAA
  GCAATGAAGACTGCTCTGCAGCAGCCAGAAGCCATTGAAAAGCCAAAAGCCTTATCCCTTATGCTGCATA
  CAGCAGATATTAGCCATCCAGCAAAAGCATGGGACCTCCATCATCGCTGGACAATGTCACTCCTGGAGGA
  GTTCTTCAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCTTTTTCTCCTCTGTGTGACCGAAAGTCC
  ACTATGGTTGCTCAGTCACAAGTAGGTTTCATTGATTTCATCGTGGAACCCACCTTCACTGTGCTTACGG
  ACATGACCGAGAAGATTGTGAGTCCATTAATCGATGAAACCTCTCAAACTGGTGGGACAGGACAGAGGCG
  TTCGAGTTTGAATAGCATCAGCTCGTCAGATGCCAAGCGATCAGGTGTCAAGACCTCTGGTTCAGAGGGA
  AGTGCCCCGATCAACAATTCTGTCATCTCCGTTGACTATAAGAGCTTTAAAGCTACTTGGACGGAAGTGG
  TGCACATCAATCGGGAGAGATGGAGGGCCAAGGTACCCAAAGAGGAGAAGGCCAAGAAGGAAGCAGAGGA
  AAAGGCTCGCCTGGCCGCAGAGGAGCAGCAAAAGGAAATGGAAGCCAAAAGCCAGGCTGAAGAAGGCGCA
  TCTGGCAAAGCTGAGAAAAAGACGTCTGGAGAAACTAAGAATCAAGTCAATGGAACACGGGCAAACAAAA
  GTGACAACCCTCGTGGGAAAAACTCCAAAGCCGAGAAGTCATCAGGAGAACAGCAACAGAATGGTGACTT
  CAAAGATGGTAAAAATAAGACAGACAAGAAGGATCACTCTAACATCGGAAATGATTCAAAGAAAACAGAT
  GGCACAAAACAGCGTTCTCACGGCTCACCAGCCCCAAGCACCAGCTCCACGTGTCGCCTTACGTTGCCAG
  TCATCAAGCCTCCTTTGCGTCATTTTAAACGCCCTGCTTACGCATCTAGCTCCTATGCACCTTCAGTCTC
  AAAGAAAACTGATGAGCATCCTGCAAGGTACAAGATGCTGGATCAGAGGATCAAAATGAAAAAGATTCAG
  AACATCTCACATAACTGGAACAGAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231386 representing NM_001191057
  Red=Cloning site Green=Tags(s)
  
  MESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT
  VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV
  ERMYRRTSNMVGLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV
  SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH
  IQTRSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIK
  AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS
  TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEG
  SAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEMEAKSQAEEGA
  SGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTD
  GTKQRSHGSPAPSTSSTCRLTLPVIKPPLRHFKRPAYASSSYAPSVSKKTDEHPARYKMLDQRIKMKKIQ
  NISHNWNRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191057
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191057.1, NP_001177986.1
• RefSeq Size: 2957 bp
• RefSeq ORF: 2130 bp
• Locus ID: 5137
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Olfactory transduction, Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'This gene encodes an enzyme that belongs to the 3'5'-cyclic nucleotide phosphodiesterase family. Members of this family catalyze hydrolysis of the cyclic nucleotides, cyclic adenosine monophosphate and cyclic guanosine monophosphate, to the corresponding nucleoside 5'-monophosphates. The enzyme encoded by this gene regulates proliferation and migration of vascular smooth muscle cells, and neointimal hyperplasia. This enzyme also plays a role in pathological vascular remodeling by regulating the stability of growth factor receptors, such as PDGF-receptor-beta. [provided by RefSeq, Jul 2016]'