FBXO11 (NM_001190274) Human Tagged ORF Clone

CAT#: RG231443

FBXO11 (GFP-tagged) - Human F-box protein 11 (FBXO11), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FBXO11
• Synonyms: FBX11; IDDFBA; PRMT9; UBR6; UG063H01; VIT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231443 representing NM_001190274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCGTCCGAGCCGCCAACCGGAGACCCAGGCGAGTGTCGCGGCCGCGCCCGGTGCAGCAACAGC
  AGCAGCAGCCCCCGCAGCAGCCGCCGCCGCAGCCGCCCCAGCAGCAGCCGCCCCAGCAGCAGCCTCCGCC
  GCCGCCGCAGCAGCAGCAGCAGCAGCAGCCTCCGCCGCCGCCACCGCCGCCTCCGCCGCTGCCTCAGGAG
  CGGAACAACGTCGGCGAGCGGGATGATGATGTGCCTGCAGATATGGTTGCAGAAGAATCAGGTCCTGGTG
  CACAAAATAGTCCATACCAACTTCGTAGAAAAACTCTTTTGCCGAAAAGAACAGCGTGTCCCACAAAGAA
  CAGTATGGAGGGCGCCTCAACTTCAACTACAGAAAACTTTGGTCATCGTGCAAAACGTGCAAGAGTGTCT
  GGAAAATCACAAGATCTATCAGCAGCACCTGCTGAACAGTATCTTCAGGAGAAACTGCCAGATGAAGTGG
  TTCTAAAAATCTTCTCTTACTTGCTGGAACAGGATCTTTGTAGAGCAGCTTGTGTATGTAAACGCTTCAG
  TGAACTTGCTAATGATCCAATTTTGTGGAAACGATTATATATGGAAGTATTTGAATATACTCGCCCTATG
  ATGCATCCTGAACCTGGAAAATTCTACCAGATTAATCCAGAAGAGTATGAACATCCAAATCCCTGGAAAG
  AGAGTTTCCAGCAGTTGTATAAAGGTGCACATGTAAAGCCAGGATTTGCTGAACATTTCTACAGTAACCC
  TGCAAGATATAAAGGAAGAGAAAATATGTTGTATTATGATACTATTGAAGATGCCCTTGGTGGGGTACAA
  GAGGCTCATTTTGATGGACTTATCTTTGTTCATTCTGGAATATATACTGATGAATGGATATATATTGAAT
  CTCCAATCACCATGATTGGTGCAGCACCTGGGAAAGTGGCAGACAAAGTTATAATTGAAAACACTAGAGA
  TTCAACCTTCGTTTTTATGGAAGGCTCTGAAGATGCTTATGTTGGATATATGACAATAAGGTTTAACCCT
  GATGACAAATCTGCACAACACCACAATGCACACCACTGCTTAGAGATTACAGTAAATTGTAGCCCTATTA
  TTGATCACTGTATCATCCGAAGTACATGTACAGTTGGTTCTGCAGTATGTGTTAGTGGTCAAGGAGCATG
  TCCCACCATCAAGCACTGTAACATCAGTGACTGTGAAAATGTTGGACTATATATAACAGATCATGCACAG
  GGAATATATGAGGATAATGAAATTTCCAATAATGCGTTAGCTGGGATTTGGGTTAAAAATCATGGAAACC
  CAATTATTAGACGGAATCATATTCATCATGGACGTGATGTTGGTGTGTTCACATTTGATCATGGCATGGG
  TTACTTTGAAAGTTGCAATATACACAGAAATAGGATAGCAGGCTTTGAAGTAAAAGCCTATGCTAACCCT
  ACAGTGGTTCGATGTGAAATTCACCATGGGCAGACTGGAGGAATATATGTCCATGAAAAAGGAAGAGGAC
  AATTCATAGAGAATAAAATCTATGCAAACAACTTTGCAGGTGTATGGATTACCTCAAATAGTGACCCAAC
  AATAAGGGGAAATTCTATATTTAATGGAAATCAAGGAGGAGTTTACATCTTTGGTGATGGACGAGGCCTT
  ATTGAAGGAAATGACATTTATGGCAATGCATTAGCAGGAATTCAAATTAGGACAAACAGTTGTCCAATTG
  TTCGGCATAACAAAATTCATGATGGCCAGCATGGTGGGATTTATGTGCATGAAAAGGGACAAGGAGTAAT
  AGAAGAGAATGAAGTTTATAGTAACACTCTAGCTGGAGTCTGGGTGACAACTGGCAGCACTCCAGTACTG
  AGAAGAAACCGGATACACAGTGGCAAGCAGGTTGGTGTTTATTTTTATGACAATGGACATGGAGTGCTAG
  AAGACAATGATATCTATAATCATATGTATTCAGGGGTTCAGATAAGGACTGGAAGCAACCCCAAAATTAG
  ACGCAACAAAATCTGGGGAGGACAGAATGGTGGAATTCTAGTTTATAATTCTGGTCTAGGCTGTATAGAA
  GACAATGAAATATTTGACAATGCAATGGCTGGAGTCTGGATTAAGACAGATAGTAATCCTACACTAAGAA
  GAAATAAAATCCATGATGGAAGAGATGGTGGCATCTGTATATTTAATGGGGGTCGAGGTCTCCTTGAAGA
  AAATGATATTTTCAGGAATGCTCAAGCAGGTGTTCTCATCAGCACTAATAGTCATCCAATCTTAAGGAAA
  AACAGAATATTTGATGGATTTGCCGCAGGTATTGAAATTACAAATCACGCAACTGCAACACTAGAAGGCA
  ATCAGATTTTTAACAACCGGTTTGGAGGCTTATTTTTAGCATCTGGTGTTAATGTGACAATGAAAGATAA
  CAAAATAATGAACAATCAAGATGCCATAGAAAAGGCTGTTAGTAGAGGCCAATGTTTATATAAAATATCA
  AGTTATACCAGCTATCCCATGCATGATTTCTACAGATGTCATACTTGTAACACCACAGATCGAAATGCCA
  TATGTGTGAACTGCATTAAGAAGTGCCATCAGGGACATGATGTAGAGTTTATTAGACATGATAGGTTTTT
  CTGTGACTGTGGTGCTGGAACACTGTCTAATCCTTGTACATTAGCTGGTGAGCCTACACATGATACAGAT
  ACACTATATGACTCTGCTCCACCTATAGAATCTAATACATTGCAGCACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231443 representing NM_001190274
  Red=Cloning site Green=Tags(s)
  
  MNSVRAANRRPRRVSRPRPVQQQQQQPPQQPPPQPPQQQPPQQQPPPPPQQQQQQQPPPPPPPPPPLPQE
  RNNVGERDDDVPADMVAEESGPGAQNSPYQLRRKTLLPKRTACPTKNSMEGASTSTTENFGHRAKRARVS
  GKSQDLSAAPAEQYLQEKLPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDPILWKRLYMEVFEYTRPM
  MHPEPGKFYQINPEEYEHPNPWKESFQQLYKGAHVKPGFAEHFYSNPARYKGRENMLYYDTIEDALGGVQ
  EAHFDGLIFVHSGIYTDEWIYIESPITMIGAAPGKVADKVIIENTRDSTFVFMEGSEDAYVGYMTIRFNP
  DDKSAQHHNAHHCLEITVNCSPIIDHCIIRSTCTVGSAVCVSGQGACPTIKHCNISDCENVGLYITDHAQ
  GIYEDNEISNNALAGIWVKNHGNPIIRRNHIHHGRDVGVFTFDHGMGYFESCNIHRNRIAGFEVKAYANP
  TVVRCEIHHGQTGGIYVHEKGRGQFIENKIYANNFAGVWITSNSDPTIRGNSIFNGNQGGVYIFGDGRGL
  IEGNDIYGNALAGIQIRTNSCPIVRHNKIHDGQHGGIYVHEKGQGVIEENEVYSNTLAGVWVTTGSTPVL
  RRNRIHSGKQVGVYFYDNGHGVLEDNDIYNHMYSGVQIRTGSNPKIRRNKIWGGQNGGILVYNSGLGCIE
  DNEIFDNAMAGVWIKTDSNPTLRRNKIHDGRDGGICIFNGGRGLLEENDIFRNAQAGVLISTNSHPILRK
  NRIFDGFAAGIEITNHATATLEGNQIFNNRFGGLFLASGVNVTMKDNKIMNNQDAIEKAVSRGQCLYKIS
  SYTSYPMHDFYRCHTCNTTDRNAICVNCIKKCHQGHDVEFIRHDRFFCDCGAGTLSNPCTLAGEPTHDTD
  TLYDSAPPIESNTLQHN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190274
• ORF Size: 2781 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190274.1, NP_001177203.1
• RefSeq Size: 4070
• RefSeq ORF: 2784
• Locus ID: 80204
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]