BORIS (CTCFL) (NM_001269055) Human Tagged ORF Clone
CAT#: RG232413
- TrueORF®
CTCFL (GFP-tagged) - Homo sapiens CCCTC-binding factor (zinc finger protein)-like (CTCFL), transcript variant 16
"NM_001269055" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CTCFL |
Synonyms | BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG232413 representing NM_001269055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACCAGTGAGAAGCCTCACC TGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTGCGGAACCATGTTAACACCCACACAGG AACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTTGTCACCAGTGGAGAACTCGTCCGACACAGG CGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCCATGTGCAAGTATGCCAGTGTGGAGGCAAGTA AATTGAAGCGCCATGTCCGATCCCACACTGGGGAGCGCCCCTTTCAGTGTTGCCAGTGCAGCTATGCCAG CAGAGATACCTACAAGCTGAAACGCCACATGAGAACGCACTCAGGTGAGAAGCCTTACGAATGCCACATC TGCCACACCCGCTTCACCCAGAGCGGGACCATGAAAATACATATTCTGCAGAAACACGGCGAAAATGTCC CCAAATACCAGTGTCCCCATTGTGCCACCATCATTGCACGGAAAAGCGACCTACGTGTGCATATGCGCAA CTTGCATGCTTACAGCGCTGCAGAGCTGAAATGCCGCTACTGTTCTGCTGTCTTCCATGAACGCTATGCC CTCATTCAGCACCAGAAAACTCATAAGAATGAGAAGAGGTTCAAGTGCAAACACTGCAGTTATGCCTGCA AGCAGGAACGTCATATGACCGCTCACATTCGTACCCACACTGGAGAGAAACCATTCACCTGCCTTTCTTG CAATAAATGTTTCCGACAGAAGCAACTTCTAAACGCTCACTTCAGGAAATACCACGATGCAAATTTCATC CCGACTGTTTACAAATGCTCCAAGTGTGGCAAAGGCTTTTCCCGCTGGGTGTTGTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232413 representing NM_001269055
Red=Cloning site Green=Tags(s) MFTSSRMSSFNRHMKTHTSEKPHLCHLCLKTFRTVTLLRNHVNTHTGTRPYKCNDCNMAFVTSGELVRHR RYKHTHEKPFKCSMCKYASVEASKLKRHVRSHTGERPFQCCQCSYASRDTYKLKRHMRTHSGEKPYECHI CHTRFTQSGTMKIHILQKHGENVPKYQCPHCATIIARKSDLRVHMRNLHAYSAAELKCRYCSAVFHERYA LIQHQKTHKNEKRFKCKHCSYACKQERHMTAHIRTHTGEKPFTCLSCNKCFRQKQLLNAHFRKYHDANFI PTVYKCSKCGKGFSRWVLY TRTRRLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001269055 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_001269055.1, NP_001255984.1 |
RefSeq Size | 2175 |
RefSeq ORF | 900 |
Locus ID | 140690 |
Protein Families | Transcription Factors |
Gene Summary | CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] |
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