APEX2 (NM_001271748) Human Tagged ORF Clone

CAT#: RG232544

APEX2 (GFP-tagged) - Homo sapiens APEX nuclease (apurinic/apyrimidinic endonuclease) 2 (APEX2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: APEX2
• Synonyms: APE2; APEXL2; XTH2; ZGRF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232544 representing NM_001271748
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTTCTATCGTTTGCTGCAAATCCGAGCAGAAGCCCTCCTGGCGGCAGGCAGCCATGTGATCATTC
  TGGGTGACCTGAATACAGCCCACCGCCCCATTGACCACTGGGATGCAGTCAACCTGGAATGCTTTGAAGA
  GGACCCAGGGCGCAAGTGGATGGACAGCTTGCTCAGTAACTTGGGGTGCCAGTCTGCCTCTCATGTAGGG
  CCCTTCATCGATAGCTACCGCTGCTTCCAACCAAAGCAGGAGGGGGCCTTCACCTGCTGGTCAGCAGTCA
  CTGGCGCCCGCCATCTCAACTATGGCTCCCGGCTTGACTATGTGCTGGGGGACAGGACCCTGGTCATAGA
  CACCTTTCAGGCCTCTTTCCTGCTGCCTGAGGTGATGGGCTCTGACCACTGCCCTGTGGGTGCAGTCTTG
  AGTGTGTCCTCTGTGCCTGCAAAACAGTGCCCACCTCTGTGCACCCGCTTCCTCCCTGAGTTTGCAGGCA
  CCCAGCTCAAGATCCTTCGCTTCCTAGTTCCTCTCGAACAAAGTCCTGTGTTGGAGCAGTCGACGCTGCA
  GCACAACAATCAAACCCGGGTACAGACATGCCAAAACAAAGCCCAAGTGCGCTCAACCAGGCCTCAGCCC
  AGTCAGGTTGGCTCTAGCAGAGGCCAGAAAAACCTGAAGAGCTACTTTCAGCCCTCCCCTAGCTGTCCCC
  AAGCCTCTCCTGACATAGAGCTGCCTAGCCTACCACTGATGAGCGCCCTCATGACCCCGAAGACTCCAGA
  AGAGAAGGCAGTGGCCAAAGTGGTGAAGGGGCAGGCCAAGACTTCAGAAGCCAAAGATGAGAAGGAGTTA
  CGGACCTCATTCTGGAAGTCTGTGCTGGCGGGGCCCTTGCGCACACCCCTCTGTGGGGGCCACAGGGAGC
  CATGTGTGATGCGTACTGTGAAGAAGCCAGGACCCAACTTGGGCCGCCGCTTCTACATGTGTGCCAGGCC
  CCGGGGTCCTCCCACTGACCCCTCCTCCCGGTGCAACTTCTTCCTCTGGAGCAGGCCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232544 representing NM_001271748
  Red=Cloning site Green=Tags(s)
  
  MRFYRLLQIRAEALLAAGSHVIILGDLNTAHRPIDHWDAVNLECFEEDPGRKWMDSLLSNLGCQSASHVG
  PFIDSYRCFQPKQEGAFTCWSAVTGARHLNYGSRLDYVLGDRTLVIDTFQASFLLPEVMGSDHCPVGAVL
  SVSSVPAKQCPPLCTRFLPEFAGTQLKILRFLVPLEQSPVLEQSTLQHNNQTRVQTCQNKAQVRSTRPQP
  SQVGSSRGQKNLKSYFQPSPSCPQASPDIELPSLPLMSALMTPKTPEEKAVAKVVKGQAKTSEAKDEKEL
  RTSFWKSVLAGPLRTPLCGGHREPCVMRTVKKPGPNLGRRFYMCARPRGPPTDPSSRCNFFLWSRPS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271748
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271748.1, NP_001258677.1
• RefSeq Size: 1914
• RefSeq ORF: 1044
• Locus ID: 27301
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]