DOPA Decarboxylase (DDC) (NM_001242888) Human Tagged ORF Clone

CAT#: RG232662

DDC (GFP-tagged) - Homo sapiens dopa decarboxylase (aromatic L-amino acid decarboxylase) (DDC), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDC
• Synonyms: AADC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232662 representing NM_001242888
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCAAGTGAATTCCGAAGGAGAGGGAAGGAGATGGTGGATTACGTGGCCAACTACATGGAAGGCA
  TTGAGGGACGCCAGGTCTACCCTGACGTGGAGCCCGGGTACCTGCGGCCGCTGATCCCTGCCGCTGCCCC
  TCAGGAGCCAGACACGTTTGAGGACATCATCAACGACGTTGAGAAGATAATCATGCCTGGGGGAAGTGCC
  AGTGAAGCCACCCTGGTGGCCCTGCTGGCCGCTCGGACCAAAGTGATCCATCGGCTGCAGGCAGCGTCCC
  CAGAGCTCACACAGGCCGCTATCATGGAGAAGCTGGTGGCTTACTCATCCGATCAGGCACACTCCTCAGT
  GGAAAGAGCTGGGTTAATTGGTGGAGTGAAATTAAAAGCCATCCCCTCAGATGGCAACTTCGCCATGCGT
  GCGTCTGCCCTGCAGGAAGCCCTGGAGAGAGACAAAGCGGCTGGCCTGATTCCTTTCTTTATGGTTGCCA
  CCCTGGGGACCACAACATGCTGCTCCTTTGACAATCTCTTAGAAGTCGGTCCTATCTGCAACAAGGAAGA
  CATATGGCTGCACGTTGATGCAGCCTACGCAGGCAGTGCATTCATCTGCCCTGAGTTCCGGCACCTTCTG
  AATGGAGTGGAGTTTGCAGATTCATTCAACTTTAATCCCCACAAATGGCTATTGGTGAATTTTGACTGTT
  CTGCCATGTGGGTGAAAAAGAGAACAGACTTAACGGGAGCCTTTAGACTGGACCCCACTTACCTGAAGCA
  CAGCCATCAGGATTCAGGGCTTATCACTGACTACCGGCATTGGCAGATACCACTGGGCAGAAGATTTCGC
  TCTTTGAAAATGTGGTTTGTATTTAGGATGTATGGAGTCAAAGGACTGCAGGCTTATATCCGCAAGCATG
  TCCAGCTGTCCCATGAGTTTGAGTCACTGGTGCGCCAGGATCCCCGCTTTGAAATCTGTGTGGAAGTCAT
  TCTGGGGCTTGTCTGCTTTCGGCTAAAGGGTTCCAACAAAGTGAATGAAGCTCTTCTGCAAAGAATAAAC
  AGTGCCAAAAAAATCCACTTGGTTCCATGTCACCTCAGGGACAAGTTTGTCCTGCGCTTTGCCATCTGTT
  CTCGCACGGTGGAATCTGCCCATGTGCAGCGGGCCTGGGAACACATCAAAGAGCTGGCGGCCGACGTGCT
  GCGAGCAGAGAGGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232662 representing NM_001242888
  Red=Cloning site Green=Tags(s)
  
  MNASEFRRRGKEMVDYVANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFEDIINDVEKIIMPGGSA
  SEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMR
  ASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLL
  NGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFR
  SLKMWFVFRMYGVKGLQAYIRKHVQLSHEFESLVRQDPRFEICVEVILGLVCFRLKGSNKVNEALLQRIN
  SAKKIHLVPCHLRDKFVLRFAICSRTVESAHVQRAWEHIKELAADVLRAERE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242888
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001242888.1, NP_001229817.1
• RefSeq Size: 1741
• RefSeq ORF: 1209
• Locus ID: 1644
• Protein Families: Druggable Genome
• Protein Pathways: Histidine metabolism, Metabolic pathways, Phenylalanine metabolism, Tryptophan metabolism, Tyrosine metabolism
• Gene Summary: The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]