ICA1 (NM_001276478) Human Tagged ORF Clone
CAT#: RG232828
- TrueORF®
ICA1 (GFP-tagged) - Homo sapiens islet cell autoantigen 1, 69kDa (ICA1), transcript variant 4
"NM_001276478" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | ICA1 |
Synonyms | ICA69; ICAp69 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG232828 representing NM_001276478
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGGACACAAATGTTATCCCTGGGACTTACAGGATCGATATGCTCAAGATAAGTCAGTTGTAAATA AGATGCAACAGAAATATTGGGAGACGAAGCAGGCCTTTATTAAAGCCACAGGGAAGAAGGAAGATGAACA TGTTGTTGCCTCTGACGCGGACCTGGATGCCAAGCTAGAGCTGTTTCATTCAATTCAGAGAACCTGTCTG GACTTATCGAAAGCAATTGTACTCTATCAAAAGAGGATATGTTTCTTGTCTCAAGAAGAAAACGAACTGG GAAAATTTCTTCGATCCCAAGGTTTCCAAGATAAAACCAGAGCAGGAAAGATGATGCAAGCGACAGGAAA GGCCCTCTGCTTTTCTTCCCAGCAAAGGTTGGCCTTACGAAATCCTTTGTGTCGATTTCACCAAGAAGTG GAGACTTTTCGGCATCGGGCCATCTCAGATACTTGGCTGACGGTGAACCGCATGGAACAGTGCAGGACGG AATATAGAGGAGCACTATTATGGATGAAGGACGTGTCTCAGGAGCTTGATCCAGACCTCTACAAGCAAAT GGAGAAGTTCAGGAAGGTACAAACACAAGTGCGCCTTGCAAAAAAAAACTTTGACAAATTGAAGATGGAT GTTTGTCAAAAAGTGGATCTTCTTGGAGCGAGCAGATGCAATCTCTTGTCTCACATGCTAGCAACATACC AGACCACTCTGCTTCATTTTTGGGAGAAAACTTCTCACACTATGGCAGCCATCCATGAGAGTTTCAAAGG TTATCAACCATATGAATTTACTACTTTAAAGAGCTTACAAGACCCTATGAAAAAATTAGTTGAGAAAGAA GAGAAGAAGAAAATCAACCAGCAGGAAAGTACAGATGCAGCCGTGCAGGAGCCGAGCCAATTAATTTCAT TAGAGGAAGAAAACCAGCGCAAGGAATCCTCTAGTTTTAAGACTGAAGATGGAAAAAGTATTTTATCTGC CTTAGACAAAGGCTCTACACATACTGCATGCTCAGGACCCATAGATGAACTATTAGACATGAAATCTGAG GAAGGTGCTTGCCTGGGACCAGTGGCAGGGACCCCGGAACCTGAAGGTGCTGACAAAGATGACCTGCTGC TGTTGAGTGAGATCTTCAATGCTTCCTCCTTGGAAGAGGGCGAGTTCAGCAAAGAGTGGGCCGCTGTGTT TGGAGACGGCCAAGTGAAGGAGCCAGTGCCCACTATGGCCCTGGGAGAGCCAGACCCCAAGGCCCAGACA GGCTCAGGTTTCCTTCCTTCGCAGCTTTTAGACCAAAATATGAAAGACTTACAGGCCTCGCTACAAGAAC CTGCTAAGGCTGCCTCAGACCTGACTGCCTGGTTCAGCCTCTTCGCTGACCTCGACCCACTCTCAAATCC TGATGCTGTTGGGAAAACCGATAAAGAACACGAATTGCTCAATGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232828 representing NM_001276478
Red=Cloning site Green=Tags(s) MSGHKCYPWDLQDRYAQDKSVVNKMQQKYWETKQAFIKATGKKEDEHVVASDADLDAKLELFHSIQRTCL DLSKAIVLYQKRICFLSQEENELGKFLRSQGFQDKTRAGKMMQATGKALCFSSQQRLALRNPLCRFHQEV ETFRHRAISDTWLTVNRMEQCRTEYRGALLWMKDVSQELDPDLYKQMEKFRKVQTQVRLAKKNFDKLKMD VCQKVDLLGASRCNLLSHMLATYQTTLLHFWEKTSHTMAAIHESFKGYQPYEFTTLKSLQDPMKKLVEKE EKKKINQQESTDAAVQEPSQLISLEEENQRKESSSFKTEDGKSILSALDKGSTHTACSGPIDELLDMKSE EGACLGPVAGTPEPEGADKDDLLLLSEIFNASSLEEGEFSKEWAAVFGDGQVKEPVPTMALGEPDPKAQT GSGFLPSQLLDQNMKDLQASLQEPAKAASDLTAWFSLFADLDPLSNPDAVGKTDKEHELLNA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001276478 |
ORF Size | 1446 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276478.1, NP_001263407.1 |
RefSeq Size | 2455 bp |
RefSeq ORF | 1449 bp |
Locus ID | 3382 |
Cytogenetics | 7p21.3 |
Protein Pathways | Type I diabetes mellitus |
Gene Summary | 'This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]' |
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