ICA1 (NM_001276478) Human Untagged Clone

CAT#: SC331064

ICA1 (untagged) - Homo sapiens islet cell autoantigen 1, 69kDa (ICA1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ICA1
• Synonyms: ICA69; ICAp69
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001276478, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCAGGACACAAATGTTATCCCTGGGACTTACAGGATCGATATGCTCAAGATAAGTCAGTTGTAAATA
  AGATGCAACAGAAATATTGGGAGACGAAGCAGGCCTTTATTAAAGCCACAGGGAAGAAGGAAGATGAACA
  TGTTGTTGCCTCTGACGCGGACCTGGATGCCAAGCTAGAGCTGTTTCATTCAATTCAGAGAACCTGTCTG
  GACTTATCGAAAGCAATTGTACTCTATCAAAAGAGGATATGTTTCTTGTCTCAAGAAGAAAACGAACTGG
  GAAAATTTCTTCGATCCCAAGGTTTCCAAGATAAAACCAGAGCAGGAAAGATGATGCAAGCGACAGGAAA
  GGCCCTCTGCTTTTCTTCCCAGCAAAGGTTGGCCTTACGAAATCCTTTGTGTCGATTTCACCAAGAAGTG
  GAGACTTTTCGGCATCGGGCCATCTCAGATACTTGGCTGACGGTGAACCGCATGGAACAGTGCAGGACGG
  AATATAGAGGAGCACTATTATGGATGAAGGACGTGTCTCAGGAGCTTGATCCAGACCTCTACAAGCAAAT
  GGAGAAGTTCAGGAAGGTACAAACACAAGTGCGCCTTGCAAAAAAAAACTTTGACAAATTGAAGATGGAT
  GTTTGTCAAAAAGTGGATCTTCTTGGAGCGAGCAGATGCAATCTCTTGTCTCACATGCTAGCAACATACC
  AGACCACTCTGCTTCATTTTTGGGAGAAAACTTCTCACACTATGGCAGCCATCCATGAGAGTTTCAAAGG
  TTATCAACCATATGAATTTACTACTTTAAAGAGCTTACAAGACCCTATGAAAAAATTAGTTGAGAAAGAA
  GAGAAGAAGAAAATCAACCAGCAGGAAAGTACAGATGCAGCCGTGCAGGAGCCGAGCCAATTAATTTCAT
  TAGAGGAAGAAAACCAGCGCAAGGAATCCTCTAGTTTTAAGACTGAAGATGGAAAAAGTATTTTATCTGC
  CTTAGACAAAGGCTCTACACATACTGCATGCTCAGGACCCATAGATGAACTATTAGACATGAAATCTGAG
  GAAGGTGCTTGCCTGGGACCAGTGGCAGGGACCCCGGAACCTGAAGGTGCTGACAAAGATGACCTGCTGC
  TGTTGAGTGAGATCTTCAATGCTTCCTCCTTGGAAGAGGGCGAGTTCAGCAAAGAGTGGGCCGCTGTGTT
  TGGAGACGGCCAAGTGAAGGAGCCAGTGCCCACTATGGCCCTGGGAGAGCCAGACCCCAAGGCCCAGACA
  GGCTCAGGTTTCCTTCCTTCGCAGCTTTTAGACCAAAATATGAAAGACTTACAGGCCTCGCTACAAGAAC
  CTGCTAAGGCTGCCTCAGACCTGACTGCCTGGTTCAGCCTCTTCGCTGACCTCGACCCACTCTCAAATCC
  TGATGCTGTTGGGAAAACCGATAAAGAACACGAATTGCTCAATGCATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276478
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276478.1, NP_001263407.1
• RefSeq Size: 2455 bp
• RefSeq ORF: 1449 bp
• Locus ID: 3382
• Cytogenetics: 7p21.3
• Protein Pathways: Type I diabetes mellitus
• Gene Summary: 'This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]'
  Transcript Variant: This variant (4) has an alternate first exon (5' UTR) and uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is one aa shorter compared to isoform a. Variants 4, 7, 8, 9, and 10 all encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.