KLHL23 (PHOSPHO2) (NM_001199290) Human Tagged ORF Clone

CAT#: RG232968

PHOSPHO2 (GFP-tagged) - Homo sapiens PHOSPHO2-KLHL23 readthrough (PHOSPHO2-KLHL23)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHOSPHO2
• Synonyms: FLJ33334
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232968 representing NM_001199290
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTAAAAGGACAAGAAGATTATATTTATCTTTTCAAGGATTCAACACATCCAGTGGATTTTCTGG
  ATGCATTCAGAACATTTTACTTGGATGGATTATTTACTGATATTACTCTTCAGTGTCCTTCAGGCATAAT
  TTTCCATTGTCACCGAGCCGTTTTAGCTGCTTGCAGCAATTATTTTAAGGCAATGTTCACAGCTGACATG
  AAAGAAAAATTTAAAAATAAAATAAAACTCTCTGGCATCCACCATGATATTCTGGAAGGCCTTGTAAATT
  ATGCATACACTTCCCAAATTGAAATAACTAAAAGAAATGTTCAAAGCCTGCTTGAGGCAGCGGATCTGCT
  ACAGTTCCTTTCAGTAAAGAAGGCTTGTGAGCGGTTTTTGGTAAGGCACTTGGATATTGATAATTGTATT
  GGAATGCACTCCTTTGCAGAATTTCATGTGTGTCCAGAACTAGAGAAGGAATCTCGAAGAATTCTATGTT
  CAAAGTTTAAGGAAGTGTGGCAACAAGAAGAATTTCTGGAAATCAGCCTTGAAAAGTTTCTCTTTATCTT
  GTCCAGAAAGAATCTCAGTGTTTGGAAAGAAGAAGCTATCATAGAGCCAGTTATTAAGTGGACTGCTCAT
  GATGTAGAAAATCGAATTGAATGCCTCTATAATCTACTGAGCTATATCAACATTGATATAGATCCAGTGT
  ACTTAAAAACAGCCTTAGGCCTTCAAAGAAGCTGCCTGCTCACCGAAAATAAGATCCGCTCCCTAATATA
  CAATGCCTTGAATCCCATGCATAAAGAGATTTCCCAGAGGTCCACAGCCACAATGTATATAATTGGAGGC
  TATTACTGGCATCCTTTATCAGAGGTTCACATATGGGATCCTTTGACAAATGTTTGGATTCAGGGAGCAG
  AAATACCAGATTATACCAGGGAGAGCTATGGTGTTACATGTTTAGGACCCAACATTTATGTAACTGGGGG
  CTACAGGACGGATAACATAGAAGCTCTTGACACAGTGTGGATCTATAACAGTGAAAGTGATGAATGGACA
  GAAGGTTTGCCAATGCTCAATGCCAGGTATTACCACTGTGCAGTCACCTTGGGTGGCTGTGTCTATGCTT
  TAGGTGGTTACAGAAAAGGGGCTCCAGCAGAAGAGGCTGAGTTCTATGATCCTTTAAAAGAGAAATGGAT
  TCCTATTGCAAACATGATTAAAGGTGTGGGAAATGCTACTGCCTGTGTCTTACATGATGTTATCTACGTC
  ATTGGTGGCCACTGTGGCTACAGAGGAAGCTGCACCTATGACAAAGTTCAGAGCTACAATTCCGATATCA
  ACGAATGGAGCCTCATCACCTCCAGTCCACATCCAGAATATGGATTGTGCTCAGTTCCGTTTGAAAATAA
  GCTCTATCTAGTCGGCGGACAAACTACAATCACAGAATGCTATGACCCTGAACAAAATGAATGGAGAGAG
  ATAGCTCCCATGATGGAAAGGAGGATGGAGTGCGGTGCCGTCATCATGAATGGATGTATTTATGTCACTG
  GAGGATACTCCTACTCAAAGGGAACGTATCTTCAGAGCATTGAGAAATATGATCCAGATCTTAATAAGTG
  GGAAATAGTGGGTAATCTTCCCAGTGCCATGCGGTCTCATGGGTGTGTTTGTGTGTATAATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232968 representing NM_001199290
  Red=Cloning site Green=Tags(s)
  
  MALKGQEDYIYLFKDSTHPVDFLDAFRTFYLDGLFTDITLQCPSGIIFHCHRAVLAACSNYFKAMFTADM
  KEKFKNKIKLSGIHHDILEGLVNYAYTSQIEITKRNVQSLLEAADLLQFLSVKKACERFLVRHLDIDNCI
  GMHSFAEFHVCPELEKESRRILCSKFKEVWQQEEFLEISLEKFLFILSRKNLSVWKEEAIIEPVIKWTAH
  DVENRIECLYNLLSYINIDIDPVYLKTALGLQRSCLLTENKIRSLIYNALNPMHKEISQRSTATMYIIGG
  YYWHPLSEVHIWDPLTNVWIQGAEIPDYTRESYGVTCLGPNIYVTGGYRTDNIEALDTVWIYNSESDEWT
  EGLPMLNARYYHCAVTLGGCVYALGGYRKGAPAEEAEFYDPLKEKWIPIANMIKGVGNATACVLHDVIYV
  IGGHCGYRGSCTYDKVQSYNSDINEWSLITSSPHPEYGLCSVPFENKLYLVGGQTTITECYDPEQNEWRE
  IAPMMERRMECGAVIMNGCIYVTGGYSYSKGTYLQSIEKYDPDLNKWEIVGNLPSAMRSHGCVCVYNV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199290
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199290.1, NP_001186219.1
• RefSeq Size: 4206
• RefSeq ORF: 1677
• Locus ID: 100526832
• Gene Summary: This locus represents naturally occurring read-through transcription between the neighboring PHOSPHO2 (phosphatase, orphan 2) and KLHL23 (kelch-like 23) genes on chromosome 2. The read-through transcript includes only non-coding PHOSPHO2 exons, and thus encodes the KLHL23 protein. [provided by RefSeq, Dec 2010]