PGM3 (NM_001199917) Human Tagged ORF Clone

CAT#: RG232979

PGM3 (GFP-tagged) - Homo sapiens phosphoglucomutase 3 (PGM3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PGM3
• Synonyms: AGM1; IMD23; PAGM; PGM 3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232979 representing NM_001199917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGAGAGTGGGGACAATCTGCTATCTGCCCTGAGTCTGCCCAGGAGTGGACATACCAAGTTGGAC
  AACATCTTGTAGACATGGATTTAGGTGCTATTACAAAATACTCAGCATTACACGCCAAGCCCAATGGACT
  GATCCTTCAATACGGGACTGCTGGATTTCGAACGAAGGCAGAACATCTTGATCATGTCATGTTTCGCATG
  GGATTATTAGCTGTCCTGAGGTCAAAACAGACAAAATCCACTATAGGAGTCATGGTAACAGCGTCCCACA
  ATCCTGAGGAAGACAATGGTGTAAAATTGGTTGATCCTTTGGGTGAAATGTTGGCACCATCCTGGGAGGA
  ACATGCCACCTGTTTAGCAAATGCTGAGGAACAAGATATGCAGAGAGTGCTTATTGACATCAGCGAGAAA
  GAAGCTGTGAATCTGCAACAAGATGCCTTTGTAGTTATTGGTAGAGATACCAGGCCCAGCAGTGAGAAAC
  TTTCACAATCTGTAATAGATGGTGTGACTGTTCTAGGAGGTCAATTCCATGATTATGGCTTGTTAACAAC
  ACCCCAGCTGCACTACATGGTGTATTGTCGAAACACGGGTGGCCGATATGGAAAGGCAACTATAGAAGGT
  TACTACCAGAAACTCTCTAAGGCTTTTGTGGAACTCACCAAACAGGCTTCTTGCAGTGGAGATGAATACA
  GATCACTTAAGGTTGACTGTGCAAATGGCATAGGGGCCCTGAAGCTAAGGGAAATGGAACACTACTTCTC
  ACAGGGCCTGTCAGTTCAGCTGTTTAATGATGGGTCCAAGGGCAAACTCAATCATTTATGTGGAGCTGAC
  TTTGTGAAAAGTCATCAGAAACCTCCACAGGGAATGGAAATTAAGTCCAATGAAAGATGCTGTTCTTTTG
  ATGGAGATGCAGACAGAATTGTTTATTACTACCATGATGCAGATGGCCACTTTCATCTCATAGATGGAGA
  CAAGATAGCAACGTTAATTAGCAGTTTCCTTAAAGAGCTCCTGGTGGAGATTGGAGAAAGTTTGAATATT
  GGTGTTGTACAAACTGCATATGCAAATGGAAGTTCAACACGGTATCTTGAAGAAGTTATGAAGGTACCTG
  TCTATTGCACTAAGACTGGTGTAAAACATTTGCACCACAAGGCTCAAGAGTTTGACATTGGAGTTTATTT
  TGAAGCAAATGGGCATGGCACTGCACTGTTTAGTACAGCTGTTGAAATGAAGATAAAACAATCAGCAGAA
  CAACTGGAAGATAAGAAAAGAAAAGCTGCTAAGATGCTTGAAAACATTATTGACTTGTTTAACCAGGCAG
  CTGGTGATGCTATTTCTGACATGCTGGTGATTGAAGCAATCTTGGCTCTGAAGGGCTTGACTGTACAACA
  GTGGGATGCTCTCTATACAGATCTTCCAAACAGACAACTTAAAGTTCAGGTTGCAGACAGGAGAGTTATT
  AGCACTACCGATGCTGAAAGACAAGCAGTTACACCCCCAGGATTACAGGAGGCAATCAATGACCTGGTGA
  AGAAGTACAAGCTTTCTCGAGCTTTTGTCCGGCCCTCTGGTACAGAAGATGTCGTCCGAGTATATGCAGA
  AGCAGACTCACAAGAAAGTGCAGATCACCTTGCACATGAAGTGAGCTTGGCAGTATTTCAGCTGGCTGGA
  GGAATTGGAGAAAGGCCCCAACCAGGTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232979 representing NM_001199917
  Red=Cloning site Green=Tags(s)
  
  MGGEWGQSAICPESAQEWTYQVGQHLVDMDLGAITKYSALHAKPNGLILQYGTAGFRTKAEHLDHVMFRM
  GLLAVLRSKQTKSTIGVMVTASHNPEEDNGVKLVDPLGEMLAPSWEEHATCLANAEEQDMQRVLIDISEK
  EAVNLQQDAFVVIGRDTRPSSEKLSQSVIDGVTVLGGQFHDYGLLTTPQLHYMVYCRNTGGRYGKATIEG
  YYQKLSKAFVELTKQASCSGDEYRSLKVDCANGIGALKLREMEHYFSQGLSVQLFNDGSKGKLNHLCGAD
  FVKSHQKPPQGMEIKSNERCCSFDGDADRIVYYYHDADGHFHLIDGDKIATLISSFLKELLVEIGESLNI
  GVVQTAYANGSSTRYLEEVMKVPVYCTKTGVKHLHHKAQEFDIGVYFEANGHGTALFSTAVEMKIKQSAE
  QLEDKKRKAAKMLENIIDLFNQAAGDAISDMLVIEAILALKGLTVQQWDALYTDLPNRQLKVQVADRRVI
  STTDAERQAVTPPGLQEAINDLVKKYKLSRAFVRPSGTEDVVRVYAEADSQESADHLAHEVSLAVFQLAG
  GIGERPQPGF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199917
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199917.1, NP_001186846.1
• RefSeq Size: 6243 bp
• RefSeq ORF: 1713 bp
• Locus ID: 5238
• Cytogenetics: 6q14.1
• Protein Pathways: Amino sugar and nucleotide sugar metabolism
• Gene Summary: 'This gene encodes a member of the phosphohexose mutase family. The encoded protein mediates both glycogen formation and utilization by catalyzing the interconversion of glucose-1-phosphate and glucose-6-phosphate. A non-synonymous single nucleotide polymorphism in this gene may play a role in resistance to diabetic nephropathy and neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]'