HERC3 (NM_001271602) Human Tagged ORF Clone

CAT#: RG233180

HERC3 (GFP-tagged) - Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 3 (HERC3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HERC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233180 representing NM_001271602
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACTGAGGATTCTGTGGCAGTGCCCAGGTTAATACAAAAGCTGAACCAGCAAACAATATTACAAG
  TTTCCTGTGGCAACTGGCATTGCTTGGCTCTTGCGGCTGATGGCCAGTTCTTCACCTGGGGAAAGAACAG
  CCATGGGCAGCTTGGCTTAGGGAAGGAGTTCCCCTCCCAAGCCAGCCCACAGAGGGTGAGGTCCCTGGAG
  GGGATCCCACTGGCTCAGGTGGCTGCCGGAGGGGCTCACAGCTTTGCCCTGTCTCTCTCAGGAGCTGTTT
  TTGGCTGGGGGATGAATAATGCCGGGCAGCTAGGGCTCAGTGATGAAAAAGATCGAGAATCTCCATGCCA
  TGTAAAACTCTTACGCACGCAAAAAGTTGTCTATATTAGTTGTGGAGAAGAACACACAGCAGTTCTCACA
  AAGAGTGGAGGTGTGTTTACCTTTGGCGCTGGTTCCTGTGGGCAACTTGGACACGACTCCATGAATGATG
  AGGTTAACCCTAGAAGAGTTCTAGAGCTGATGGGTAGTGAAGTAACTCAAATTGCTTGTGGCAGACAACA
  TACCCTAGCCTTCGTGCCTTCTTCTGGACTCATCTATGCATTTGGTTGTGGAGCAAGAGGTCAATTAGGA
  ACTGGGCACACTTGTAATGTTAAGTGCCCATCTCCTGTCAAGGGTTACTGGGCTGCCCACAGTGGCCAGC
  TTTCAGCCCGAGCTGATCGCTTTAAATATCATATCGTTAAGCAGATCTTCTCTGGAGGAGACCAGACTTT
  TGTACTTTGCTCCAAATACGAGAATTATTCTCCTGCTGTTGACTTCAGGACTATGAACCAAGCACATTAT
  ACCAGTTTAATAAATGATGAAACCATAGCAGTTTGGAGACAAAAACTCTCAGAACACAACAATGCAAATA
  CAATCAATGGTGTTGTTCAGATATTATCTTCTGCAGCCTGTTGGAATGGAAGTTTTCTTGAAAAAAAAAT
  TGATGAACATTTTAAAACGAGTCCCAAAATCCCTGGGATTGACCTGAACTCAACTAGGGTGTTATTTGAG
  AAGTTAATGAACTCTCAGCACTCCATGATTCTAGAACAGATTTTGAACAGTTTTGAAAGTTGTCTGATTC
  CCCAGTTGTCAAGCTCACCACCAGATGTTGAAGCCATGAGAATCTATTTAATACTACCTGAGTTTCCCCT
  ACTCCAGGATTCCAAGTATTATATAACATTGACTATTCCCTTGGCTATGGCCATTCTTCGGCTGGATACA
  AACCCCAGCAAAGTACTAGATAACTGGTGGTCTCAGGTATGCCCGAAATATTTCATGAAGCTGGTAAACC
  TCTATAAAGGTGCAGTCCTTTATCTACTGAGGGGAAGAAAGACATTCTTAATTCCCGTACTGTTTAACAA
  TTATATCACAGCAGCTCTCAAACTCTTGGAGAAGTTATATAAGGTAAATCTTAAAGTGAAGCATGTGGAA
  TATGATACATTTTACATTCCTGAGATTTCCAATCTCGTGGACATTCAGGAAGACTACCTCATGTGGTTCT
  TGCATCAAGCAGGGATGAAGGCTAGACCATCAATAATACAGGATACTGTAACACTTTGTTCCTACCCTTT
  CATCTTTGATGCCCAAGCCAAGACCAAAATGTTACAGACAGATGCTGAACTACAGATGCAGGTGGCAGTC
  AATGGAGCCAACCTGCAGAATGTCTTCATGCTTCTCACCCTGGAGCCTCTGCTGGCCAGAAGCCCCTTCC
  TGGTCCTTCACGTTCGCAGGAACAACCTTGTTGGAGATGCCCTAAGAGAGCTGAGCATTCATTCTGATAT
  TGATTTGAAAAAGCCTCTCAAAGTAATCTTTGATGGTGAAGAAGCAGTGGATGCCGGTGGTGTTACAAAG
  GAATTTTTTCTTTTGCTGTTAAAAGAACTTTTGAATCCCATCTATGGAATGTTTACCTACTATCAAGATT
  CAAATCTCTTGTGGTTTTCAGACACGTGTTTTGTAGAGCACAACTGGTTTCACTTGATTGGTATAACCTG
  TGGACTAGCTATCTACAACTCCACTGTGGTCGATCTCCACTTCCCATTGGCTCTCTACAAGAAGTTACTC
  AATGTAAAGCCTGGCTTGGAAGACTTAAAGGAGTTGTCACCCACTGAAGGAAGGAGTCTCCAAGAGCTTT
  TAGATTACCCCGGGGAGGATGTGGAGGAGACTTTCTGCCTCAACTTCACGATCTGCCGAGAAAGCTATGG
  AGTGATTGAACAGAAGAAGCTGATACCTGGGGGAGATAATGTAACTGTGTGCAAGGATAACAGGCAGGAA
  TTTGTGGATGCTTATGTGAATTATGTCTTCCAAATCTCAGTTCATGAATGGTACACAGCCTTCTCTAGTG
  GCTTCCTAAAGGTGTGTGGTGGCAAAGTACTTGAGCTCTTCCAGCCTTCAGAACTGAGGGCTATGATGGT
  GGGGAACAGCAACTACAACTGGGAAGAACTGGAAGAGACTGCCATCTACAAGGGAGATTACTCGGCCACA
  CATCCCACTGTAAAACTATTTTGGGAAACATTTCATGAGTTTCCATTGGAAAAGAAGAAGAAGTTTCTCT
  TGTTCCTGACAGGCAGCGATCGGATTCCCATCTACGGCATGGCCAGTCTGCAGATTGTCATCCAGTCCAC
  AGCCAGCGGGGAGGAGTACTTGCCGGTGGCCCACACTTGCTACAACCTTCTTGACCTCCCCAAGTACAGC
  AGCAAAGAGATTCTGAGTGCCCGGCTGACCCAGGCCCTTGACAACTATGAAGGGTTTAGTTTGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233180 representing NM_001271602
  Red=Cloning site Green=Tags(s)
  
  MTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLE
  GIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRESPCHVKLLRTQKVVYISCGEEHTAVLT
  KSGGVFTFGAGSCGQLGHDSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLG
  TGHTCNVKCPSPVKGYWAAHSGQLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAVDFRTMNQAHY
  TSLINDETIAVWRQKLSEHNNANTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFE
  KLMNSQHSMILEQILNSFESCLIPQLSSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDT
  NPSKVLDNWWSQVCPKYFMKLVNLYKGAVLYLLRGRKTFLIPVLFNNYITAALKLLEKLYKVNLKVKHVE
  YDTFYIPEISNLVDIQEDYLMWFLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAV
  NGANLQNVFMLLTLEPLLARSPFLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTK
  EFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEHNWFHLIGITCGLAIYNSTVVDLHFPLALYKKLL
  NVKPGLEDLKELSPTEGRSLQELLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQE
  FVDAYVNYVFQISVHEWYTAFSSGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELEETAIYKGDYSAT
  HPTVKLFWETFHEFPLEKKKKFLLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYS
  SKEILSARLTQALDNYEGFSLA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271602
• ORF Size: 2796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271602.1, NP_001258531.1
• RefSeq Size: 4730
• RefSeq ORF: 2799
• Locus ID: 8916
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]