PHOSPHO2 (NM_001199286) Human Tagged ORF Clone

CAT#: RG233522

PHOSPHO2 (GFP-tagged) - Homo sapiens phosphatase, orphan 2 (PHOSPHO2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHOSPHO2
• Synonyms: MGC22679; MGC111048
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233522 representing NM_001199286
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATTTTGCTAGTTTTTGACTTTGACAATACAATCATAGATGACAATAGTGACACTTGGATTGTAC
  AATGTGCTCCCAACAAAAAGCTTCCTATTGAACTACGTGATTCTTATCGAAAAGGATTTTGGACAGAATT
  TATGGGCAGAGTCTTTAAGTATTTGGGAGATAAGGGTGTAAGAGAACATGAAATGAAAAGAGCAGTGACA
  TCATTGCCTTTCACTCCAGGGATGGTGGAACTCTTCAACTTTATAAGAAAGAATAAGGATAAATTTGACT
  GCATTATTATTTCAGATTCAAATTCGGTCTTCATAGATTGGGTTTTAGAAGCTGCCAGTTTTCATGACAT
  ATTTGATAAAGTGTTTACAAATCCAGCAGCTTTTAATAGCAATGGTCATCTCACTGTTGAAAATTATCAT
  ACTCATTCTTGCAATAGATGCCCAAAGAATCTTTGCAAAAAGGTAGTTTTGATAGAATTTGTAGATAAAC
  AGTTACAACAGGGAGTGAATTATACACAAATTGTTTATATTGGTGATGGTGGAAATGATGTCTGTCCAGT
  CACCTTTTTAAAGAATGATGATGTTGCCATGCCACGGAAAGGATATACCTTACAGAAAACTCTTTCCAGA
  ATGTCTCAAAATCTTGAGCCTATGGAATATTCTGTTGTAGTTTGGTCCTCAGGTGTTGATATAATTTCTC
  ATTTACAATTTCTAATAAAGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233522 representing NM_001199286
  Red=Cloning site Green=Tags(s)
  
  MKILLVFDFDNTIIDDNSDTWIVQCAPNKKLPIELRDSYRKGFWTEFMGRVFKYLGDKGVREHEMKRAVT
  SLPFTPGMVELFNFIRKNKDKFDCIIISDSNSVFIDWVLEAASFHDIFDKVFTNPAAFNSNGHLTVENYH
  THSCNRCPKNLCKKVVLIEFVDKQLQQGVNYTQIVYIGDGGNDVCPVTFLKNDDVAMPRKGYTLQKTLSR
  MSQNLEPMEYSVVVWSSGVDIISHLQFLIKD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199286
• ORF Size: 723 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001199286.1, NP_001186215.1
• RefSeq Size: 1268
• RefSeq ORF: 726
• Locus ID: 493911
• Gene Summary: Phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). Also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate. [UniProtKB/Swiss-Prot Function]