PTCRA (NM_001243169) Human Tagged ORF Clone

CAT#: RG233565

PTCRA (GFP-tagged) - Homo sapiens pre T-cell antigen receptor alpha (PTCRA), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTCRA
• Synonyms: PT-ALPHA; PTA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233565 representing NM_001243169
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGTACATGGCTGCTACTTCTCCTGGCCCTTGGGTGTCCAGCCCTACCCACAGGTCCTGTTTCCT
  TCCCTTCCTCACCAGAGGCTGCCACAACAGGCCCCATCTGGTTCTCAGCCGGCAATGGCAGTGCACTGGA
  TGCCTTCACCTATGGCCCTTCCCCAGCAACGGATGGCACCTGGACCAACTTGGCCCATCTCTCCCTGCCT
  TCTGAGGAGCTGGCATCCTGGGAGCCTTTGGTCTGCCACACTGGGCCTGGGGCTGAGGGTCACAGCAGGA
  GTACACAGCCCATGCATCTGTCAGGAGAGGCTTCTACAGCCAGGACCTGCCCCCAGGAGCCTCTCAGGGG
  GACACCGGGTGGGGCGCTGTGGCTGGGGGTCCTGCGGCTGCTGCTCTTCAAGCTGCTGCTGTTTGACCTG
  CTCCTGACCTGCAGCTGCCTGTGCGACCCCGCGGGCCCGCTGCCTTCCCCCGCAACCACCACCCGCCTGC
  GAGCCCTCGGCTCCCATCGACTGCACCCGGCCACGGAGACTGGGGGACGAGAGGCCACCAGCTCACCCAG
  ACCCCAGCCTCGGGACCGCCGCTGGGGTGACACCCCTCCGGGTCGGAAGCCCGGGAGCCCAGTATGGGGG
  GAAGGGTCTTACCTCAGCAGTTACCCCACTTGCCCAGCACAGGCCTGGTGCTCAAGATCTGCCCTCAGGG
  CTCCTTCCTCCAGTCTTGGAGCATTTTTTGCAGGTGACCTGCCTCCTCCTCTGCAGGCTGGAGCTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233565 representing NM_001243169
  Red=Cloning site Green=Tags(s)
  
  MAGTWLLLLLALGCPALPTGPVSFPSSPEAATTGPIWFSAGNGSALDAFTYGPSPATDGTWTNLAHLSLP
  SEELASWEPLVCHTGPGAEGHSRSTQPMHLSGEASTARTCPQEPLRGTPGGALWLGVLRLLLFKLLLFDL
  LLTCSCLCDPAGPLPSPATTTRLRALGSHRLHPATETGGREATSSPRPQPRDRRWGDTPPGRKPGSPVWG
  EGSYLSSYPTCPAQAWCSRSALRAPSSSLGAFFAGDLPPPLQAGAA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243169
• ORF Size: 768 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001243169.1, NP_001230098.1
• RefSeq Size: 1022
• RefSeq ORF: 771
• Locus ID: 171558
• Protein Families: Transmembrane
• Protein Pathways: Notch signaling pathway
• Gene Summary: The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]