ISY1 (NM_001204890) Human Tagged ORF Clone

CAT#: RG233787

ISY1 (GFP-tagged) - Homo sapiens ISY1-RAB43 readthrough (ISY1-RAB43)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ISY1
• Synonyms: ISY1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233787 representing NM_001204890
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGAAATGCAGAAAAGGCCATGACGGCCTTAGCAAGATTTCGCCAGGCTCAGCTGGAAGAGGGAA
  AAGTGAAGGAACGAAGACCCTTTCTGGCCTCAGAATGTACTGAACTGCCTAAAGCTGAGAAGTGGAGACG
  ACAGATCATTGGAGAGATCTCTAAAAAAGTGGCTCAGATTCAGAATGCTGGTTTAGGTGAATTTCGAATT
  CGTGACCTGAATGATGAAATTAACAAGCTGCTAAGGGAGAAAGGACACTGGGAGGTCCGGATAAAGGAGC
  TGGGAGGTCCTGATTATGGAAAAGTTGGCCCTAAAATGCTGGATCATGAAGGAAAAGAAGTCCCAGGAAA
  CCGAGGTTACAAGTACTTTGGAGCAGCAAAAGATTTGCCTGGTGTTAGAGAGCTGTTTGAAAAAGAACCT
  CTTCCTCCTCCCAGAAAGACACGTGCTGAGCTCATGAAGGCAATCGATTTTGAGTACTATGGTTACCTAG
  ATGAAGATGATGGTGTTATTGTGCCTTTGGAACAGGAATATGAAAAGAAACTCAGAGCCGAGTTAGTGGA
  AAAGTGGAAAGCAGAGAGAGAGGCTCGGCTGGCAAGAGGAGAAAAGGAAGAGGAGGAGGAAGAGGAGGAA
  GAGATCAACATCTATGCAGTCACCGAGGAGGAGTCGGACGAGGAAGGCAGCCAGGAGAAAGGAGGGGACG
  ACAGCCAGCAGAAGTTCATTGCTCACGTCCCTGTTCCCTCGCAGCAAGAGATTGAGGAGGCACTGGTGCG
  AAGGAAGAAAATGGAACTCCTCCAGAAGTATGCAAGCGAGACCCTGCAGGCCCAAAGTGAAGAAGCCAGA
  AGGCTCCTGGGCTGCAGATCTGGGACACGGCCGGCCAGGAGCGGTTCCGCACCATCACCCAGAGCTACTA
  CCGCAGTGCCAATGGGGCCATCCTTGCCTACGACATCACCAAGAGGAGCTCCTTCCTGTCGGTGCCTCAC
  TGGATTGAGGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233787 representing NM_001204890
  Red=Cloning site Green=Tags(s)
  
  MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRI
  RDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEP
  LPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKWKAEREARLARGEKEEEEEEEE
  EINIYAVTEEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAR
  RLLGCRSGTRPARSGSAPSPRATTAVPMGPSLPTTSPRGAPSCRCLTGLRM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204890
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001204890.1, NP_001191819.1
• RefSeq Size: 5068
• RefSeq ORF: 996
• Locus ID: 100534599
• Gene Summary: This locus represents naturally occurring read-through transcription between the neighboring ISY1 (ISY1 splicing factor homolog) and RAB43 (RAB43, member RAS oncogene family) gene on chromosome 3. The read-through transcript encodes a protein that shares sequence identity with the upstream gene product, but its C-terminus is distinct due to a frameshift relative to the downstream gene. [provided by RefSeq, Mar 2011]