Apoptosis inhibitor 5 (API5) (NM_001243747) Human Tagged ORF Clone

CAT#: RG233788

API5 (GFP-tagged) - Homo sapiens apoptosis inhibitor 5 (API5), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: API5
• Synonyms: AAC-11; AAC11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233788 representing NM_001243747
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACAGTAGAGGAGCTTTACCGCAATTATGGCATCCTGGCCGATGCCACGGAGCAAGTGGGCCAGC
  ATAAAGATGCCTATCAAGTGATATTGGATGGTGTGAAAGGTGGTACTAAGGAAAAGCGATTAGCAGCTCA
  ATTTATTCCGAAATTCTTTAAGCATTTTCCAGAATTGGCTGATTCTGCTATCAATGCACAGTTAGACCTC
  TGTGAGGATGAAGATGTATCTATTCGACGTCAAGCAATTAAAGAACTGCCTCAATTTGCCACTGGAGAAA
  ATCTTCCTCGAGTGGCAGATATACTAACGCAACTTTTGCAGACAGATGACTCTGCAGAATTTAACCTAGT
  GAACAATGCCCTATTAAGTATATTTAAAATGGAAAAACTAGAAACAAATTTAAGGAAACTATTTGATAAG
  TTATTGGAATACATGCCCCTCCCTCCAGAAGAGGCAGAAAATGGAGAGAATGCTGGTAATGAAGAACCCA
  AGCTACAGTTCAGTTATGTGGAATGTTTGTTGTACAGTTTTCACCAGTTGGGCCGAAAACTTCCAGATTT
  CTTAACAGCCAAACTGAATGCAGAAAAGCTCAAAGATTTCAAAATCAGGCTGCAGTACTTTGCACGGGGC
  CTGCAAGTTTATATCAGACAACTTCGCTTAGCTCTCCAGGGTAAAACGGGTGAGGCCTTAAAAACAGAAG
  AGAACAAGATTAAAGTCGTTGCATTGAAAATAACAAACAATATCAATGTTTTAATCAAGGATCTCTTCCA
  CATTCCTCCTTCTTATAAGAGCACAGTAACACTATCCTGGAAACCTGTACAAAAGGTTGAGATTGGGCAA
  AAGAGAGCCAGTGAAGATACAACTTCAGGTTCACCACCCAAGAAATCTTCAGCAGGACCAAAAAGAGATG
  CCAGGCAGATTTATAACCCTCCCAGTGGGAAATATAGCAGCAATTTGGGCAACTTTAATTATGAGAGGAG
  CCTTCAGGGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233788 representing NM_001243747
  Red=Cloning site Green=Tags(s)
  
  MPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAINAQLDL
  CEDEDVSIRRQAIKELPQFATGENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMEKLETNLRKLFDK
  LLEYMPLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARG
  LQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQ
  KRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYERSLQGK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001243747
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001243747.1, NP_001230676.1
• RefSeq Size: 3257
• RefSeq ORF: 996
• Locus ID: 8539
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]