EGR3 (NM_001199880) Human Tagged ORF Clone

CAT#: RG233839

EGR3 (GFP-tagged) - Homo sapiens early growth response 3 (EGR3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EGR3
• Synonyms: EGR-3; PILOT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233839 representing NM_001199880
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCATGTGCGGCGTGGAGTCCCCGCGGTGGGAGAGAGAATGTAATGGACATCGGTCTGACCAACG
  AGAAGCCCAACCCGGAACTCTCTTACTCCGGCTCCTTCCAGCCAGCCCCCGGCAACAAGACCGTGACCTA
  CTTGGGAAAGTTCGCCTTCGACTCCCCTTCCAACTGGTGCCAGGACAACATCATTAGCCTCATGAGCGCC
  GGCATCTTGGGGGTGCCCCCGGCTTCAGGGGCGCTCAGCACGCAGACGTCCACGGCCAGCATGGTGCAGC
  CACCGCAGGGTGACGTGGAGGCCATGTATCCCGCGCTACCCCCCTACTCCAACTGCGGCGACCTCTACTC
  AGAGCCCGTGTCTTTCCACGACCCCCAGGGCAATCCCGGGCTCGCCTATTCCCCCCAGGATTACCAATCG
  GCCAAGCCGGCGTTGGACAGCAATCTCTTCCCCATGATTCCTGACTACAACCTCTACCACCACCCCAACG
  ACATGGGCTCCATTCCGGAGCACAAGCCCTTCCAGGGCATGGACCCCATCCGGGTCAACCCGCCCCCTAT
  TACCCCTCTGGAGACCATCAAGGCATTCAAAGACAAGCAGATCCACCCGGGCTTTGGCAGCCTGCCCCAG
  CCGCCGCTCACCCTCAAGCCCATCCGGCCCCGCAAGTACCCCAACCGGCCTAGCAAGACACCGCTCCACG
  AACGGCCCCACGCGTGCCCGGCCGAGGGCTGCGACCGCCGTTTCAGCCGTTCGGACGAGCTGACCCGGCA
  CCTGCGCATCCACACGGGCCACAAGCCCTTCCAGTGCCGGATCTGCATGCGGAGCTTCAGCCGCAGCGAC
  CACCTCACCACTCACATCCGCACTCATACGGGCGAGAAGCCCTTTGCCTGCGAGTTCTGCGGGCGCAAGT
  TTGCGCGCAGCGACGAGCGCAAGCGCCACGCCAAGATCCACCTCAAGCAAAAGGAGAAGAAGGCGGAGAA
  GGGCGGTGCACCCTCTGCATCCTCGGCGCCCCCCGTGTCGCTGGCCCCCGTGGTCACCACCTGCGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233839 representing NM_001199880
  Red=Cloning site Green=Tags(s)
  
  MEPCAAWSPRGGRENVMDIGLTNEKPNPELSYSGSFQPAPGNKTVTYLGKFAFDSPSNWCQDNIISLMSA
  GILGVPPASGALSTQTSTASMVQPPQGDVEAMYPALPPYSNCGDLYSEPVSFHDPQGNPGLAYSPQDYQS
  AKPALDSNLFPMIPDYNLYHHPNDMGSIPEHKPFQGMDPIRVNPPPITPLETIKAFKDKQIHPGFGSLPQ
  PPLTLKPIRPRKYPNRPSKTPLHERPHACPAEGCDRRFSRSDELTRHLRIHTGHKPFQCRICMRSFSRSD
  HLTTHIRTHTGEKPFACEFCGRKFARSDERKRHAKIHLKQKEKKAEKGGAPSASSAPPVSLAPVVTTCA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199880
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199880.1, NP_001186809.1
• RefSeq Size: 3918 bp
• RefSeq ORF: 1050 bp
• Locus ID: 1960
• Cytogenetics: 8p21.3
• Gene Summary: 'This gene encodes a transcriptional regulator that belongs to the EGR family of C2H2-type zinc-finger proteins. It is an immediate-early growth response gene which is induced by mitogenic stimulation. The protein encoded by this gene participates in the transcriptional regulation of genes in controling biological rhythm. It may also play a role in a wide variety of processes including muscle development, lymphocyte development, endothelial cell growth and migration, and neuronal development. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]'