Corticotropin Releasing Factor Receptor 2 (CRHR2) (NM_001202482) Human Tagged ORF Clone

CAT#: RG234014

CRHR2 (GFP-tagged) - Homo sapiens corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CRHR2
• Synonyms: CRF-RB; CRF2; CRFR2; HM-CRF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234014 representing NM_001202482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCGGCACTGCTCCACAGCCTGCTGGAGGCCAACTGCAGCCTGGCGCTGGCTGAAGAGCTGCTCT
  TGGACGGCTGGGGGCCACCCCTGGACCCCGAGGGTCCCTACTCCTACTGCAACACGACCTTGGACCAGAT
  CGGAACGTGCTGGCCCCGCAGCGCTGCCGGAGCCCTCGTGGAGAGGCCGTGCCCCGAGTACTTCAACGGC
  GTCAAGTACAACACGACCCGGAATGCCTATCGAGAATGCTTGGAGAATGGGACGTGGGCCTCAAAGATCA
  ACTACTCACAGTGTGAGCCCATTTTGGATGACAAGAGGAAGTATGACCTGCACTACCGCATCGCCCTTGT
  CGTCAACTACCTGGGCCACTGCGTATCTGTGGCAGCCCTGGTGGCCGCCTTCCTGCTTTTCCTGGCCCTG
  CGGAGCATTCGCTGTCTGCGGAATGTGATTCACTGGAACCTCATCACCACCTTTATCCTGCGAAATGTCA
  TGTGGTTCCTGCTGCAGCTCGTTGACCATGAAGTGCACGAGAGCAATGAGGTCTGGTGCCGCTGCATCAC
  CACCATCTTCAACTACTTCGTGGTGACCAACTTCTTCTGGATGTTTGTGGAAGGCTGCTACCTGCACACG
  GCCATTGTCATGACCTACTCCACTGAGCGCCTGCGCAAGTGCCTCTTCCTCTTCATCGGATGGTGCATCC
  CCTTCCCCATCATCGTCGCCTGGGCCATCGGCAAGCTCTACTATGAGAATGAACAGTGCTGGTTTGGCAA
  GGAGCCTGGCGACCTGGTGGACTACATCTACCAAGGCCCCATCATTCTCGTGCTCCTGATCAATTTCGTA
  TTTCTGTTCAACATCGTCAGGATCCTAATGACAAAGTTACGCGCGTCCACCACATCCGAGACAATCCAGT
  ACAGGAAGGCAGTGAAGGCCACCCTGGTGCTCCTGCCCCTCCTGGGCATCACCTACATGCTCTTCTTCGT
  CAATCCCGGGGAGGACGACCTGTCACAGATCATGTTCATCTATTTCAACTCCTTCCTGCAGTCGTTCCAG
  GGTTTCTTCGTGTCTGTCTTCTACTGCTTCTTCAATGGAGAGGTGCGCTCAGCCGTGAGGAAGAGGTGGC
  ACCGCTGGCAGGACCATCACTCCCTTCGAGTCCCCATGGCCCGGGCCATGTCCATCCCTACATCACCCAC
  ACGGATCAGCTTCCACAGCATCAAGCAGACGGCCGCTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234014 representing NM_001202482
  Red=Cloning site Green=Tags(s)
  
  MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNG
  VKYNTTRNAYRECLENGTWASKINYSQCEPILDDKRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLAL
  RSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESNEVWCRCITTIFNYFVVTNFFWMFVEGCYLHT
  AIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINFV
  FLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQ
  GFFVSVFYCFFNGEVRSAVRKRWHRWQDHHSLRVPMARAMSIPTSPTRISFHSIKQTAAV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001202482
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202482.1, NP_001189411.1
• RefSeq Size: 2995 bp
• RefSeq ORF: 1233 bp
• Locus ID: 1395
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011]'