PKMYT1 (NM_001258451) Human Tagged ORF Clone

CAT#: RG234263

PKMYT1 (GFP-tagged) - Homo sapiens protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PKMYT1
• Synonyms: MYT1; PPP1R126
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234263 representing NM_001258451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATGCCCACGGAGGGCACCCCGCCACCTCTGAGTGGCACCCCCATCCCAGTCCCAGCCTACTTCC
  GCCACGCAGAACCTGGATTCTCCCTCAAGAGGCCCAGGGGGCTCAGCCGGAGCCTCCCACCTCCGCCCCC
  TGCCAAGGGCAGCATTCCCATCAGCCGCCTCTTCCCTCCTCGGACCCCAGGCTGGCACCAGCTGCAGCCC
  CGGCGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGCAGAGCCCTGGGTATGACCCAAGCCGGCCAG
  AGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCCTACGGAGAGGTCTTCAA
  GGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAAAGCGTTCCATGTCACCATTCCGGGGCCCCAAG
  GACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGAAGGTGGGGCAGCACCCATGCTGCGTGCGGC
  TGGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCAGACGGAGCTGTGCGGGCCCAGCCTGCAGCA
  ACACTGTGAGGCCTGGGGTGCCAGCCTGCCTGAGGCCCAGGTCTGGGGCTACCTGCGGGACACGCTGCTT
  GCCCTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTGATGTCAAGCCTGCCAACATCTTCCTGGGGC
  CCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGTGGAGCTGGGTACAGCAGGAGCTGGTGAGGT
  CCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTGCAGGGCTCCTATGGGACAGCAGCGGATGTG
  TTCAGTCTGGGCCTCACCATCCTGGAAGTGGCATGCAACATGGAGCTGCCCCACGGTGGGGAGGGCTGGC
  AGCAGCTGCGCCAGGGCTACCTGCCCCCTGAGTTCACTGCCGGTCTGTCTTCCGAGCTGCGTTCTGTCCT
  TGTCATGATGCTGGAGCCAGACCCCAAGCTGCGGGCCACGGCCGAGGCCCTGCTGGCACTGCCTGTGTTG
  AGGCAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAGCGGAGGCCCTGAGCCGAGGGTGGGCCCTGT
  GGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGGGCTGGCTCACCCTGCCAGCTGGCTACAGCC
  CCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGCAGTTTGCTCCTGGACAGCAGCCTCTCCAGC
  AACTGGGATGACGACAGCCTAGGGCCTTCACTCTCCCCTGAGGCTGTCCTGGCCCGGACTGTGGGGAGCA
  CCTCCACCCCCCGGAGCAGGTGCACACCCAGGGATGCCCTGGACCTAAGTGACATCAACTCAGAGCCTCC
  TCGGGGCTCCTTCCCCTCCTTTGAGCCTCGGAACCTCCTCAGCCTGTTTGAGGACACCCTAGACCCAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234263 representing NM_001258451
  Red=Cloning site Green=Tags(s)
  
  MPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQP
  RRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPK
  DRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLL
  ALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADV
  FSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVL
  RQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSS
  NWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258451
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258451.1, NP_001245380.1
• RefSeq Size: 2051
• RefSeq ORF: 1473
• Locus ID: 9088
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]