Leukotriene A4 hydrolase (LTA4H) (NM_001256644) Human Tagged ORF Clone
CAT#: RG234316
- TrueORF®
LTA4H (GFP-tagged) - Homo sapiens leukotriene A4 hydrolase (LTA4H), transcript variant 3
"NM_001256644" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | LTA4H |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234316 representing NM_001256644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCACAGAGAAATCTTTCTAAAAGACAAGTACCGACCATGCATATCCCAGTTAAGACTCGTCGAC TTCTTGCAGCCTTAAAGGTTTTGGATACAAAGGACCTTACAATAGAAAAAGTAGTGATCAATGGACAAGA AGTCAAATATGCTCTTGGAGAAAGACAAAGTTACAAGGGATCGCCAATGGAAATCTCTCTTCCTATCGCT TTGAGCAAAAATCAAGAAATTGTTATAGAAATTTCTTTTGAGACCTCTCCAAAATCTTCTGCTCTCCAGT GGCTCACTCCTGAACAGACTTCTGGGAAGGAACACCCATATCTCTTTAGTCAGTGCCAGGCCATCCACTG CAGAGCAATCCTTCCTTGTCAGGACACTCCTTCTGTGAAATTAACCTATACTGCAGAGGTGTCTGTCCCT AAAGAACTGGTGGCACTTATGAGTGCTATTCGTGATGGAGAAACACCTGACCCAGAAGACCCAAGCAGGA AAATATACAAATTCATCCAAAAAGTTCCAATACCCTGCTACCTGATTGCTTTAGTTGTTGGAGCTTTAGA AAGCAGGCAAATTGGCCCAAGAACTTTGGTGTGGTCTGAGAAAGAGCAGGTGGAAAAGTCTGCTTATGAG TTTTCTGAGACTGAATCTATGCTTAAAATAGCAGAAGATCTGGGAGGACCGTATGTATGGGGACAGTATG ACCTATTGGTCCTGCCACCATCCTTCCCTTATGGTGGCATGGAGAATCCTTGCCTTACTTTTGTAACTCC TACTCTACTGGCAGGCGACAAGTCACTCTCCAATGTCATTGCACATGAAATATCTCATAGCTGGACAGGG AATCTAGTGACCAACAAAACTTGGGATCACTTTTGGTTAAATGAGGGACATACTGTGTACTTGGAACGCC ACATTTGCGGACGATTGTTTGGTGAAAAGTTCAGACATTTTAATGCTCTGGGAGGATGGGGAGAACTACA GAATTCGGTAAAGACATTTGGGGAGACACATCCTTTCACCAAACTTGTGGTTGATCTGACAGATATAGAC CCTGATGTAGCTTATTCTTCAGTTCCCTATGAGAAGGGCTTTGCTTTACTTTTTTACCTTGAACAACTGC TTGGAGGACCAGAGATTTTCCTAGGATTCTTAAAAGCTTATGTTGAGAAGTTTTCCTATAAGAGCATAAC TACTGATGACTGGAAGGATTTCCTGTATTCCTATTTTAAAGATAAGGTTGATGTTCTCAATCAAGTTGAT TGGAATGCCTGGCTCTACTCTCCTGGACTGCCTCCCATAAAGCCCAATTATGATATGACTCTGACAAATG CTTGTATTGCCTTAAGTCAAAGATGGATTACTGCCAAAGAAGATGATTTAAATTCATTCAATGCCACAGA CCTGAAGGATCTCTCTTCTCATCAATTGAATGAGTTTTTAGCACAGACGCTCCAGAGGATGGCTGCGGCT CTGCATTCAATCCAAGTGGGAGGACGCAATTCCTTTGGCGCTAAAGATGGCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234316 representing NM_001256644
Red=Cloning site Green=Tags(s) MLPQRNLSKRQVPTMHIPVKTRRLLAALKVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIA LSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVP KELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSAYE FSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTG NLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDID PDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVD WNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRMAAA LHSIQVGGRNSFGAKDGN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001256644 |
ORF Size | 1524 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001256644.1, NP_001243573.1 |
RefSeq Size | 2062 bp |
RefSeq ORF | 1527 bp |
Locus ID | 4048 |
Cytogenetics | 12q23.1 |
Protein Families | Druggable Genome, Protease |
Protein Pathways | Arachidonic acid metabolism, Metabolic pathways |
Gene Summary | 'The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]' |
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