Leukotriene A4 hydrolase (LTA4H) (NM_001256644) Human Tagged ORF Clone

CAT#: RC234316

LTA4H (Myc-DDK tagged) - Homo sapiens leukotriene A4 hydrolase (LTA4H), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LTA4H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234316 representing NM_001256644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCACAGAGAAATCTTTCTAAAAGACAAGTACCGACCATGCATATCCCAGTTAAGACTCGTCGAC
  TTCTTGCAGCCTTAAAGGTTTTGGATACAAAGGACCTTACAATAGAAAAAGTAGTGATCAATGGACAAGA
  AGTCAAATATGCTCTTGGAGAAAGACAAAGTTACAAGGGATCGCCAATGGAAATCTCTCTTCCTATCGCT
  TTGAGCAAAAATCAAGAAATTGTTATAGAAATTTCTTTTGAGACCTCTCCAAAATCTTCTGCTCTCCAGT
  GGCTCACTCCTGAACAGACTTCTGGGAAGGAACACCCATATCTCTTTAGTCAGTGCCAGGCCATCCACTG
  CAGAGCAATCCTTCCTTGTCAGGACACTCCTTCTGTGAAATTAACCTATACTGCAGAGGTGTCTGTCCCT
  AAAGAACTGGTGGCACTTATGAGTGCTATTCGTGATGGAGAAACACCTGACCCAGAAGACCCAAGCAGGA
  AAATATACAAATTCATCCAAAAAGTTCCAATACCCTGCTACCTGATTGCTTTAGTTGTTGGAGCTTTAGA
  AAGCAGGCAAATTGGCCCAAGAACTTTGGTGTGGTCTGAGAAAGAGCAGGTGGAAAAGTCTGCTTATGAG
  TTTTCTGAGACTGAATCTATGCTTAAAATAGCAGAAGATCTGGGAGGACCGTATGTATGGGGACAGTATG
  ACCTATTGGTCCTGCCACCATCCTTCCCTTATGGTGGCATGGAGAATCCTTGCCTTACTTTTGTAACTCC
  TACTCTACTGGCAGGCGACAAGTCACTCTCCAATGTCATTGCACATGAAATATCTCATAGCTGGACAGGG
  AATCTAGTGACCAACAAAACTTGGGATCACTTTTGGTTAAATGAGGGACATACTGTGTACTTGGAACGCC
  ACATTTGCGGACGATTGTTTGGTGAAAAGTTCAGACATTTTAATGCTCTGGGAGGATGGGGAGAACTACA
  GAATTCGGTAAAGACATTTGGGGAGACACATCCTTTCACCAAACTTGTGGTTGATCTGACAGATATAGAC
  CCTGATGTAGCTTATTCTTCAGTTCCCTATGAGAAGGGCTTTGCTTTACTTTTTTACCTTGAACAACTGC
  TTGGAGGACCAGAGATTTTCCTAGGATTCTTAAAAGCTTATGTTGAGAAGTTTTCCTATAAGAGCATAAC
  TACTGATGACTGGAAGGATTTCCTGTATTCCTATTTTAAAGATAAGGTTGATGTTCTCAATCAAGTTGAT
  TGGAATGCCTGGCTCTACTCTCCTGGACTGCCTCCCATAAAGCCCAATTATGATATGACTCTGACAAATG
  CTTGTATTGCCTTAAGTCAAAGATGGATTACTGCCAAAGAAGATGATTTAAATTCATTCAATGCCACAGA
  CCTGAAGGATCTCTCTTCTCATCAATTGAATGAGTTTTTAGCACAGACGCTCCAGAGGATGGCTGCGGCT
  CTGCATTCAATCCAAGTGGGAGGACGCAATTCCTTTGGCGCTAAAGATGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234316 representing NM_001256644
  Red=Cloning site Green=Tags(s)
  
  MLPQRNLSKRQVPTMHIPVKTRRLLAALKVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIA
  LSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDTPSVKLTYTAEVSVP
  KELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSEKEQVEKSAYE
  FSETESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISHSWTG
  NLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFGETHPFTKLVVDLTDID
  PDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVD
  WNAWLYSPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRMAAA
  LHSIQVGGRNSFGAKDGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256644
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256644.1, NP_001243573.1
• RefSeq Size: 2062 bp
• RefSeq ORF: 1527 bp
• Locus ID: 4048
• Cytogenetics: 12q23.1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• MW: 57.7 kDa
• Gene Summary: 'The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]'