MAK (NM_001242957) Human Tagged ORF Clone

CAT#: RG234651

MAK (GFP-tagged) - Homo sapiens male germ cell-associated kinase (MAK), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAK
• Synonyms: RP62
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234651 representing NM_001242957
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGATACACAACCATGAGACAGTTGGGGGACGGCACGTATGGGAGTGTGCTTATGGGCAAGAGTA
  ATGAATCCGGGGAGCTGGTGGCCATCAAAAGGATGAAGAGAAAGTTCTATTCTTGGGATGAATGCATGAA
  CTTGAGAGAAGTTAAGTCTCTGAAGAAACTTAATCATGCCAATGTTATTAAATTGAAAGAAGTTATCAGA
  GAAAATGACCATCTTTATTTTATATTTGAATATATGAAAGAAAACCTCTATCAATTAATGAAAGACAGAA
  ACAAGTTGTTCCCTGAATCAGTCATCAGAAATATTATGTATCAAATATTGCAAGGGCTGGCTTTTATCCA
  TAAACATGGCTTTTTTCATAGGGACATGAAACCAGAAAACTTGCTTTGTATGGGTCCAGAGCTTGTGAAA
  ATTGCTGATTTTGGACTTGCAAGAGAATTAAGGTCACAGCCACCATACACTGATTATGTATCTACCAGAT
  GGTATCGTGCCCCTGAAGTTTTACTGAGATCTTCAGTTTATAGTTCTCCCATTGATGTGTGGGCTGTTGG
  AAGTATCATGGCTGAACTCTATATGTTAAGGCCACTTTTCCCAGGGACAAGTGAGGTCGATGAAATCTTT
  AAAATTTGCCAAGTTTTAGGGACTCCCAAAAAAAGTGACTGGCCAGAAGGATACCAGCTGGCATCCTCTA
  TGAACTTCCGTTTTCCCCAGTGTGTTCCTATAAACTTAAAAACTCTTATTCCCAATGCCAGTAATGAAGC
  TATTCAGCTCATGACCGAAATGTTGAATTGGGATCCAAAGAAACGACCGACAGCAAGCCAGGCATTGAAA
  CACCCATATTTTCAAGTTGGTCAGGTATTAGGCCCTTCGTCAAATCATCTGGAATCAAAACAGTCTTTAA
  ATAAGCAGCTGCAACCATTAGAATCAAAGCCATCTTTAGTTGAGGTAGAGCCTAAGCCTCTGCCGGATAT
  AATCGATCAGGTTGTTGGACAACCCCAGCCAAAAACTAGCCAGCAGCCACTGCAGCCCATTCAGCCGCCA
  CAGAACCTGAGCGTCCAGCAACCTCCAAAGCAACAGAGTCAGGAGAAACCGCCACAAACGCTATTCCCGA
  GCATCGTCAAAAACATGCCAACTAAGCCAAATGGCACACTGAGTCATAAAAGTGGTAGGAGGCGTTGGGG
  TCAGACTATCTTCAAGTCTGGAGATAGCTGGGAAGAGTTGGAGGACTATGATTTCGGAGCCTCCCATTCC
  AAGAAGCCAAGCATGGGTGTTTTTAAAGAAAAAAGGAAAAAAGATTCTCCATTTCGGCTTCCAGAGCCAG
  TACCCTCAGGCTCCAACCACTCGACAGGGGAAAACAAGAGCTTACCTGCTGTTACTTCCCTAAAATCTGA
  TTCCGAATTGTCAACTGCTCCAACCTCTAAACAGTACTACTTGAAACAATCAAGATATCTTCCAGGTGTG
  AATCCCAAGAAGGTGTCCTTGATAGCCAGTGGAAAGGAAATAAACCCCCACACTTGGAGCAACCAGTTAT
  TCCCCAAGTCACTGGGACCCGTTGGGGCAGAACTTGCTTTCAAAAGGAGCAATGCAGAAGAAAGCATAAT
  TAAACCAATCGAAAAACTATCATGCAATGAAACTTTTCCTGAAAAATTAGAGGACCCACAAGGAAATCTT
  GGAAGTTATGCTACTTACAATCAGTCAGGATATATTCCTTCCTTTCTCAAAAAAGAAGTGCAGTCAGCTG
  GCCAGAGGATCCACTTAGCACCTCTCAATGCAACGGCTTCAGAATATACCTGGAACACAAAAACTGGTCG
  GGGGCAGTTTTCAGGACGTACTTATAATCCTACAGCAAAAAACCTAAATATTGTGAACCGTGCACAGCCC
  ATTCCCTCAGTGCATGGGAGGACAGACTGGGTGGCCAAGTATGGAGGCCACCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234651 representing NM_001242957
  Red=Cloning site Green=Tags(s)
  
  MNRYTTMRQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHANVIKLKEVIR
  ENDHLYFIFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHGFFHRDMKPENLLCMGPELVK
  IADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSVYSSPIDVWAVGSIMAELYMLRPLFPGTSEVDEIF
  KICQVLGTPKKSDWPEGYQLASSMNFRFPQCVPINLKTLIPNASNEAIQLMTEMLNWDPKKRPTASQALK
  HPYFQVGQVLGPSSNHLESKQSLNKQLQPLESKPSLVEVEPKPLPDIIDQVVGQPQPKTSQQPLQPIQPP
  QNLSVQQPPKQQSQEKPPQTLFPSIVKNMPTKPNGTLSHKSGRRRWGQTIFKSGDSWEELEDYDFGASHS
  KKPSMGVFKEKRKKDSPFRLPEPVPSGSNHSTGENKSLPAVTSLKSDSELSTAPTSKQYYLKQSRYLPGV
  NPKKVSLIASGKEINPHTWSNQLFPKSLGPVGAELAFKRSNAEESIIKPIEKLSCNETFPEKLEDPQGNL
  GSYATYNQSGYIPSFLKKEVQSAGQRIHLAPLNATASEYTWNTKTGRGQFSGRTYNPTAKNLNIVNRAQP
  IPSVHGRTDWVAKYGGHR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242957
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242957.1, NP_001229886.1
• RefSeq Size: 3974 bp
• RefSeq ORF: 1947 bp
• Locus ID: 4117
• Cytogenetics: 6p24.2
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'