EPB41L1 (NM_001258330) Human Tagged ORF Clone

CAT#: RG234742

EPB41L1 (GFP-tagged) - Homo sapiens erythrocyte membrane protein band 4.1-like 1 (EPB41L1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EPB41L1
• Synonyms: 4.1N; MRD11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234742 representing NM_001258330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCTTGGGGAGGATCAATGAGGTAGAACCTGCAAAGGGCTTAGCAGAGAGCCTAGCACCTACTG
  AGCGCTCAGTAAAGAGCCTAGACATGGAGGAGAAGGACTACAGTGAGGCCGATGGCCTTTCGGAGAGGAC
  CACGCCCAGCAAGGCCCAGAAATCGCCCCAGAAGATTGCCAAGAAATACAAGAGTGCCATCTGCCGGGTC
  ACTCTGCTTGATGCCTCGGAGTATGAGTGTGAGGTGGAGAAACATGGCCGGGGCCAGGTGCTGTTTGACC
  TGGTCTGTGAACACCTCAACCTCCTAGAGAAGGACTACTTCGGCCTGACCTTCTGTGATGCTGACAGCCA
  GAAGAACTGGCTGGACCCCTCCAAGGAGATCAAGAAGCAGATCCGGAGTAGCCCCTGGAATTTTGCCTTC
  ACAGTCAAGTTCTACCCGCCTGATCCTGCCCAGCTGACAGAAGACATCACAAGATACTACCTGTGCCTGC
  AGCTGCGGGCAGACATCATCACGGGCCGGCTGCCATGCTCCTTTGTCACGCATGCCCTACTGGGCTCCTA
  CGCTGTGCAGGCTGAGCTGGGTGACTATGATGCTGAGGAGCATGTGGGCAACTATGTCAGCGAGCTCCGC
  TTCGCCCCTAACCAGACCCGGGAGCTGGAGGAGAGGATCATGGAGCTGCATAAGACATATAGGGGGATGA
  CCCCGGGAGAAGCAGAAATCCACTTCTTAGAGAATGCCAAGAAGCTTTCCATGTACGGAGTAGACCTGCA
  CCATGCCAAGGACTCTGAGGGCATCGACATCATGTTAGGCGTTTGTGCCAATGGCCTGCTCATCTACCGG
  GACCGGCTGAGAATCAACCGCTTTGCCTGGCCCAAGATCCTCAAGATCTCCTACAAGAGGAGTAACTTCT
  ATATCAAGATCCGGCCTGGGGAGTATGAGCAATTTGAGAGCACAATTGGCTTTAAGCTCCCAAACCACCG
  GTCAGCCAAGAGACTGTGGAAGGTCTGCATCGAGCATCATACATTCTTCCGGCTGGTGTCCCCTGAGCCC
  CCACCCAAGGGCTTCCTGGTGATGGGCTCCAAGTTCCGGTACAGTGGGAGGACCCAGGCACAGACTCGCC
  AGGCCAGCGCCCTCATTGACCGGCCTGCACCCTTCTTTGAGCGTTCTTCCAGCAAACGGTACACCATGTC
  CCGCAGCCTTGATGGAGCAGAGTTCTCCCGCCCAGCCTCGGTCAGCGAGAACCATGATGCAGGGCCTGAC
  GGTGACAAGCGGGATGAGGATGGCGAGTCTGGGGGGCAACGGTCAGAGGCTGAGGAGGGAGAGGTCAGGA
  CTCCAACCAAGATCAAGGAGCTAAAGTTCTTAGACAAGCCAGAAGATGTCTTGCTGAAGCACCAGGCCAG
  CATCAATGAGCTCAAAAGGACCCTGAAGGAGCCCAACAGCAAACTCATCCACCGGGATCGAGACTGGGAA
  CGGGAGCGCAGGCTGCCCTCCTCCCCCGCCTCCCCCTCCCCCAAGGGCACCCCTGAGAAAGCCAATGAGC
  CCGTGAAAACAGAAACCATGACTGTCAGCAGTCTGGCCATTAGAAAGAAGATTGAGCCGGAGGCCGTACT
  GCAGACCAGAGTCTCCGCTATGGATAACACCCAGCAGGTTGATGGGAGTGCCTCAGTGGGGAGGGAGTTC
  ATAGCAACCACTCCCTCCATCACCACGGAGACCATATCGACCACCATGGAGAACAGTCTCAAGTCCGGGA
  AGGGGGCAGCTGCCATGATCCCAGGCCCACAGACGGTGGCCACGGAAATCCGTTCTCTTTCTCCGATCAT
  CGGGAAAGATGTCCTCACCAGCACCTACGGCGCCACTGCGGAAACCCTCTCAACCTCCACCACCACCCAT
  GTCACCAAAACTGTGAAAGGAGGGTTTTCTGAGACAAGGATCGAGAAGCGAATCATCATTACTGGGGATG
  AAGATGTCGATCAAGACCAGGCCCTGGCTTTGGCCATCAAGGAGGCCAAACTGCAGCATCCTGATATGCT
  GGTAACCAAAGCTGTCGTATACAGAGAAACAGACCCATCCCCAGAGGAGAGGGACAAGAAGCCACAGGAA
  TCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234742 representing NM_001258330
  Red=Cloning site Green=Tags(s)
  
  MVFLGRINEVEPAKGLAESLAPTERSVKSLDMEEKDYSEADGLSERTTPSKAQKSPQKIAKKYKSAICRV
  TLLDASEYECEVEKHGRGQVLFDLVCEHLNLLEKDYFGLTFCDADSQKNWLDPSKEIKKQIRSSPWNFAF
  TVKFYPPDPAQLTEDITRYYLCLQLRADIITGRLPCSFVTHALLGSYAVQAELGDYDAEEHVGNYVSELR
  FAPNQTRELEERIMELHKTYRGMTPGEAEIHFLENAKKLSMYGVDLHHAKDSEGIDIMLGVCANGLLIYR
  DRLRINRFAWPKILKISYKRSNFYIKIRPGEYEQFESTIGFKLPNHRSAKRLWKVCIEHHTFFRLVSPEP
  PPKGFLVMGSKFRYSGRTQAQTRQASALIDRPAPFFERSSSKRYTMSRSLDGAEFSRPASVSENHDAGPD
  GDKRDEDGESGGQRSEAEEGEVRTPTKIKELKFLDKPEDVLLKHQASINELKRTLKEPNSKLIHRDRDWE
  RERRLPSSPASPSPKGTPEKANEPVKTETMTVSSLAIRKKIEPEAVLQTRVSAMDNTQQVDGSASVGREF
  IATTPSITTETISTTMENSLKSGKGAAAMIPGPQTVATEIRSLSPIIGKDVLTSTYGATAETLSTSTTTH
  VTKTVKGGFSETRIEKRIIITGDEDVDQDQALALAIKEAKLQHPDMLVTKAVVYRETDPSPEERDKKPQE
  S
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258330
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258330.1, NP_001245259.1
• RefSeq Size: 5745 bp
• RefSeq ORF: 2106 bp
• Locus ID: 2036
• Cytogenetics: 20q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• Gene Summary: 'Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]'