BCAR3 (NM_001261410) Human Tagged ORF Clone

CAT#: RG234795

BCAR3 (GFP-tagged) - Homo sapiens breast cancer anti-estrogen resistance 3 (BCAR3), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BCAR3
• Synonyms: AND-34; NSP2; SH2D3B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234795 representing NM_001261410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAAGGAATGCAGTGCTTTCCACGCCCTCTCAGCAGCTCTGTGCTGCTTCTATCACCGCAAGTCTT
  TCATTGGAGTCAAGTTCTCCAAGGAGAGGCACATCATGGACAGGACCCCCGAGAAACTGAAGAAGGAGCT
  GGAGGAGGAGCTGCTCCTGAGCAGCGAGGACCTGCGCAGCCATGCCTGGTACCACGGCCGCATCCCCCGA
  CAGGTGTCTGAAAACCTTGTGCAGCGAGATGGTGACTTCCTAGTTCGTGACTCTCTGTCCAGCCCTGGGA
  ACTTTGTCCTGACCTGTCAGTGGAAGAACCTCGCTCAGCACTTCAAAATCAACCGGACAGTTCTGCGACT
  CAGCGAGGCCTACAGCCGCGTGCAGTACCAGTTCGAGATGGAGAGCTTCGACTCCATCCCCGGCCTGGTG
  CGCTGCTACGTGGGCAACCGCCGGCCCATCTCCCAGCAGAGTGGCGCCATCATCTTCCAGCCCATCAACA
  GGACGGTGCCTCTGCGGTGCCTGGAGGAGCATTATGGCACCTCCCCAGGCCAGGCCCGGGAGGGCAGCCT
  CACCAAGGGAAGGCCGGATGTGGCCAAGAGGCTGAGCCTCACCATGGGTGGCGTCCAGGCCCGAGAGCAG
  AATTTGCCCAGGGGAAACCTCCTCAGAAACAAAGAAAAGAGTGGTAGCCAGCCCGCCTGCCTGGATCACA
  TGCAGGACAGAAGAGCCTTGTCCCTCAAAGCCCACCAGTCAGAGAGCTACCTGCCGATTGGCTGCAAGCT
  GCCACCTCAGTCCTCGGGTGTGGACACAAGCCCCTGCCCAAACTCACCTGTGTTCAGGACGGGAAGCGAG
  CCTGCCCTGAGCCCAGCAGTGGTTCGGAGGGTCTCCTCAGACGCCAGGGCTGGGGAGGCGCTGAGGGGAT
  CAGACAGTCAACTGTGCCCTAAGCCCCCGCCTAAGCCCTGCAAGGTGCCGTTCCTCAAGGTTCCCTCGTC
  TCCCTCTGCCTGGCTCAACTCAGAGGCCAACTACTGTGAACTGAACCCAGCGTTTGCCACAGGCTGCGGC
  AGGGGAGCAAAGCTACCCTCATGTGCCCAGGGAAGCCACACAGAACTGCTCACAGCCAAGCAGAATGAGG
  CGCCAGGTCCCCGGAACTCTGGCGTCAACTACTTGATCCTTGATGATGATGACAGGGAAAGACCTTGGGA
  ACCTGCGGCAGCTCAGATGGAGAAGGGGCAGTGGGACAAGGGCGAGTTTGTGACGCCCCTCCTGGAGACT
  GTCTCCTCCTTCAGGCCCAACGAGTTTGAGTCAAAGTTCCTTCCCCCTGAGAATAAGCCCCTGGAAACAG
  CAATGTTGAAACGTGCAAAAGAACTGTTCACCAACAACGACCCCAAGGTCATCGCCCAGCACGTACTGAG
  CATGGACTGCAGGGTTGCTAGGATACTTGGAGTCTCTGAAGAGATGAGGAGGAACATGGGGGTGAGCTCA
  GGCCTGGAACTCATTACCTTGCCTCACGGACACCAGCTGCGCCTGGACATAATTGAAAGACACAACACAA
  TGGCCATCGGCATTGCAGTGGACATTCTGGGATGCACGGGCACTTTGGAGGACCGAGCGGCCACTCTGAG
  TAAGATCATCCAGGTGGCGGTGGAACTGAAGGATTCCATGGGGGACCTCTATTCCTTCTCAGCTCTCATG
  AAAGCCCTGGAAATGCCACAGATCACAAGGTTAGAAAAGACGTGGACTGCTCTGCGGCACCAGTACACCC
  AAACTGCCATTCTCTATGAGAAACAGCTGAAGCCCTTCAGCAAACTCCTGCATGAAGGCAGAGAGTCCAC
  ATGTGTTCCCCCAAACAATGTATCAGTCCCACTGCTGATGCCGCTTGTGACGTTAATGGAGCGCCAGGCT
  GTGACTTTTGAAGGAACCGACATGTGGGAAAAAAACGACCAGAGCTGTGAAATCATGCTGAACCATTTGG
  CAACAGCGCGATTCATGGCCGAGGCTGCAGACAGCTACCGGATGAATGCTGAGAGGATCCTGGCAGGTTT
  TCAACCAGATGAAGAAATGAATGAAATCTGCAAGACTGAATTTCAAATGCGATTGCTATGGGGCAGCAAA
  GGTGCACAAGTCAATCAGACAGAGAGATATGAGAAATTCAACCAGATTTTAACTGCCCTCTCGCGTAAAT
  TGGAACCTCCTCCTGTAAAGCAGGCAGAGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234795 representing NM_001261410
  Red=Cloning site Green=Tags(s)
  
  MPKECSAFHALSAALCCFYHRKSFIGVKFSKERHIMDRTPEKLKKELEEELLLSSEDLRSHAWYHGRIPR
  QVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSEAYSRVQYQFEMESFDSIPGLV
  RCYVGNRRPISQQSGAIIFQPINRTVPLRCLEEHYGTSPGQAREGSLTKGRPDVAKRLSLTMGGVQAREQ
  NLPRGNLLRNKEKSGSQPACLDHMQDRRALSLKAHQSESYLPIGCKLPPQSSGVDTSPCPNSPVFRTGSE
  PALSPAVVRRVSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPSSPSAWLNSEANYCELNPAFATGCG
  RGAKLPSCAQGSHTELLTAKQNEAPGPRNSGVNYLILDDDDRERPWEPAAAQMEKGQWDKGEFVTPLLET
  VSSFRPNEFESKFLPPENKPLETAMLKRAKELFTNNDPKVIAQHVLSMDCRVARILGVSEEMRRNMGVSS
  GLELITLPHGHQLRLDIIERHNTMAIGIAVDILGCTGTLEDRAATLSKIIQVAVELKDSMGDLYSFSALM
  KALEMPQITRLEKTWTALRHQYTQTAILYEKQLKPFSKLLHEGRESTCVPPNNVSVPLLMPLVTLMERQA
  VTFEGTDMWEKNDQSCEIMLNHLATARFMAEAADSYRMNAERILAGFQPDEEMNEICKTEFQMRLLWGSK
  GAQVNQTERYEKFNQILTALSRKLEPPPVKQAEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001261410
• ORF Size: 2202 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001261410.1, NP_001248339.1
• RefSeq Size: 2893
• RefSeq ORF: 2205
• Locus ID: 8412
• Protein Families: Druggable Genome
• Gene Summary: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]