MCM3 (NM_001270472) Human Tagged ORF Clone

CAT#: RG234929

MCM3 (GFP-tagged) - Homo sapiens minichromosome maintenance complex component 3 (MCM3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MCM3
• Synonyms: HCC5; P1-MCM3; P1.h; RLFB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234929 representing NM_001270472
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTATATGCTCCCAAGGTCCCCGCCTCTTCCTCGAGGTAACCTTTGGTGGCGGGAAGAGTTCGGAAG
  TTTTCGCGCCGGGGTGGAGTCATCCTGGGAACCTCCACGCGACTTTGGTGGAGGTAGTTCTTTGGCAGCG
  GGCATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCC
  TGGACGACGAGGCTTCTGAACAATGCCTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTG
  TGGCCTCCATTGATGCTACCTATGCCAAGCAGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGG
  CTCCAAGCACGTCTCCCCGCGGACTCTTACCTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATT
  GTCACTAAATGTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCTACTAAGAAGA
  CCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGCCTTTCCCTCCAGCTCTGTCTATCCTACCAA
  GGATGAGGAGAACAATCCCCTTGAGACAGAATATGGCCTTTCTGTCTACAAGGATCACCAGACCATCACC
  ATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCAGCTCCCCCGCTCTGTGGACGTCATTCTGGATGATG
  ACTTGGTGGATAAAGCGAAGCCTGGTGACCGGGTTCAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAA
  GAAGGGAGGCTACACCTCTGGGACCTTCAGGACTGTCCTGATTGCCTGTAATGTTAAGCAGATGAGCAAG
  GATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGCCAAGATCAAGAAGTTCAGTAAAACCCGATCCAAGG
  ATATCTTTGACCAGCTGGCCAAGTCATTGGCCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAAT
  CCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCCACATCCGTGGGGACATCAAT
  ATTCTTCTAATAGGAGACCCATCCGTTGCCAAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCC
  GAGCTATCCCCACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCTGTCACCACAGACCAGGA
  AACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTGGCTGACCGAGGCGTGGTTTGCATTGATGAA
  TTTGACAAAATGTCTGACATGGATCGCACAGCCATCCATGAAGTGATGGAGCAGGGTCGAGTGACCATTG
  CCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGCTGCAGTGTTTTGGCAGCTGCCAACCCTGTCTACGG
  CAGGTATGACCAGTATAAGACTCCAATGGAGAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGAC
  TTGCTCTTCATCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAGATCTCAGACCATGTCCTTCGGA
  TGCACCGTTACAGAGCACCTGGGGAGCAGGATGGCGATGCTATGCCCTTGGGTAGTGCTGTGGATATCCT
  GGCCACAGATGATCCCAACTTTAGCCAGGAAGATCAGCAGGACACCCAGATTTATGAGAAGCATGACAAC
  CTTCTACATGGGACCAAGAAGAAAAAGGAGAAGATGGTGAGTGCAGCATTCATGAAGAAGTACATCCATG
  TGGCCAAAATCATCAAGCCTGTCCTGACACAGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCT
  GCGCAGCCAGGATAGCATGAGCTCAGACACCGCCAGGACATCTCCAGTTACAGCCCGAACACTGGAAACT
  CTGATTCGACTGGCCACAGCCCATGCGAAGGCCCGCATGAGCAAGACTGTGGACCTGCAGGATGCAGAGG
  AAGCTGTGGAGTTGGTCCAGTATGCTTACTTTAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCG
  AAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAAAGCCAAGAGGACCAGGAGCAGAAGAGGAAG
  AGAAGGAAGACTCGCCAGCCAGATGCCAAAGATGGGGATTCATACGACCCCTATGACTTCAGTGACACAG
  AGGAGGAAATGCCTCAAGTACACACTCCAAAGACGGCAGACTCACAGGAGACCAAGGAATCCCAGAAAGT
  GGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGGTGGCCCTCTTGGATGTGTTCCGGGAAGCTCATGCG
  CAGTCAATCGGCATGAATCGCCTCACAGAATCCATCAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTG
  AGATCCAGGCTGCTCTGAGCAAGATGCAGGATGACAATCAGGTCATGGTGTCTGAGGGCATCATCTTCCT
  CATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234929 representing NM_001270472
  Red=Cloning site Green=Tags(s)
  
  MLICSQGPRLFLEVTFGGGKSSEVFAPGWSHPGNLHATLVEVVLWQRAWRVPWCWTMWSCGRLREITWTS
  WTTRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGI
  VTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTIT
  IQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSK
  DAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDIN
  ILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDE
  FDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFD
  LLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDN
  LLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLET
  LIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRK
  RRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHA
  QSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270472
• ORF Size: 2454 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270472.1, NP_001257401.1
• RefSeq Size: 3121 bp
• RefSeq ORF: 2289 bp
• Locus ID: 4172
• Cytogenetics: 6p12.2
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: 'The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]'