MCM3 (NM_001270472) Human Untagged Clone

CAT#: SC332953

MCM3 (untagged) - Homo sapiens minichromosome maintenance complex component 3 (MCM3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM3
• Synonyms: HCC5; P1-MCM3; P1.h; RLFB
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001270472, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTTATATGCTCCCAAGGTCCCCGCCTCTTCCTCGAGGTAACCTTTGGTGGCGGGAAGAGTTCGGAAG
  TTTTCGCGCCGGGGTGGAGTCATCCTGGGAACCTCCACGCGACTTTGGTGGAGGTAGTTCTTTGGCAGCG
  GGCATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCC
  TGGACGACGAGGCTTCTGAACAATGCCTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTG
  TGGCCTCCATTGATGCTACCTATGCCAAGCAGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGG
  CTCCAAGCACGTCTCCCCGCGGACTCTTACCTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATT
  GTCACTAAATGTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCTACTAAGAAGA
  CCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGCCTTTCCCTCCAGCTCTGTCTATCCTACCAA
  GGATGAGGAGAACAATCCCCTTGAGACAGAATATGGCCTTTCTGTCTACAAGGATCACCAGACCATCACC
  ATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCAGCTCCCCCGCTCTGTGGACGTCATTCTGGATGATG
  ACTTGGTGGATAAAGCGAAGCCTGGTGACCGGGTTCAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAA
  GAAGGGAGGCTACACCTCTGGGACCTTCAGGACTGTCCTGATTGCCTGTAATGTTAAGCAGATGAGCAAG
  GATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGCCAAGATCAAGAAGTTCAGTAAAACCCGATCCAAGG
  ATATCTTTGACCAGCTGGCCAAGTCATTGGCCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAAT
  CCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCCACATCCGTGGGGACATCAAT
  ATTCTTCTAATAGGAGACCCATCCGTTGCCAAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCC
  GAGCTATCCCCACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCTGTCACCACAGACCAGGA
  AACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTGGCTGACCGAGGCGTGGTTTGCATTGATGAA
  TTTGACAAAATGTCTGACATGGATCGCACAGCCATCCATGAAGTGATGGAGCAGGGTCGAGTGACCATTG
  CCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGCTGCAGTGTTTTGGCAGCTGCCAACCCTGTCTACGG
  CAGGTATGACCAGTATAAGACTCCAATGGAGAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGAC
  TTGCTCTTCATCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAGATCTCAGACCATGTCCTTCGGA
  TGCACCGTTACAGAGCACCTGGGGAGCAGGATGGCGATGCTATGCCCTTGGGTAGTGCTGTGGATATCCT
  GGCCACAGATGATCCCAACTTTAGCCAGGAAGATCAGCAGGACACCCAGATTTATGAGAAGCATGACAAC
  CTTCTACATGGGACCAAGAAGAAAAAGGAGAAGATGGTGAGTGCAGCATTCATGAAGAAGTACATCCATG
  TGGCCAAAATCATCAAGCCTGTCCTGACACAGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCT
  GCGCAGCCAGGATAGCATGAGCTCAGACACCGCCAGGACATCTCCAGTTACAGCCCGAACACTGGAAACT
  CTGATTCGACTGGCCACAGCCCATGCGAAGGCCCGCATGAGCAAGACTGTGGACCTGCAGGATGCAGAGG
  AAGCTGTGGAGTTGGTCCAGTATGCTTACTTTAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCG
  AAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAAAGCCAAGAGGACCAGGAGCAGAAGAGGAAG
  AGAAGGAAGACTCGCCAGCCAGATGCCAAAGATGGGGATTCATACGACCCCTATGACTTCAGTGACACAG
  AGGAGGAAATGCCTCAAGTACACACTCCAAAGACGGCAGACTCACAGGAGACCAAGGAATCCCAGAAAGT
  GGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGGTGGCCCTCTTGGATGTGTTCCGGGAAGCTCATGCG
  CAGTCAATCGGCATGAATCGCCTCACAGAATCCATCAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTG
  AGATCCAGGCTGCTCTGAGCAAGATGCAGGATGACAATCAGGTCATGGTGTCTGAGGGCATCATCTTCCT
  CATCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270472
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270472.1, NP_001257401.1
• RefSeq Size: 3121 bp
• RefSeq ORF: 2457 bp
• Locus ID: 4172
• Cytogenetics: 6p12.2
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: 'The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]'
  Transcript Variant: This variant (2) lacks an alternate exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.