EPB41L1 (NM_001258329) Human Tagged ORF Clone

CAT#: RG234991

EPB41L1 (GFP-tagged) - Homo sapiens erythrocyte membrane protein band 4.1-like 1 (EPB41L1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EPB41L1
• Synonyms: 4.1N; MRD11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234991 representing NM_001258329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACAGAGACAGGCCCCGACTCTGAGGTGAAGAAAGCTCAGGAGGAGGCCCCGCAGCAGCCCGAGG
  CTGCTGCCGCTGTGACCACCCCTGTGACCCCTGCAGGCCACGGCCACCCAGAGGCCAACTCCAATGAGAA
  GCATCCATCCCAGCAGGACACGCGGCCTGCTGAACAGAGCCTAGACATGGAGGAGAAGGACTACAGTGAG
  GCCGATGGCCTTTCGGAGAGGACCACGCCCAGCAAGGCCCAGAAATCGCCCCAGAAGATTGCCAAGAAAT
  ACAAGAGTGCCATCTGCCGGGTCACTCTGCTTGATGCCTCGGAGTATGAGTGTGAGGTGGAGAAACATGG
  CCGGGGCCAGGTGCTGTTTGACCTGGTCTGTGAACACCTCAACCTCCTAGAGAAGGACTACTTCGGCCTG
  ACCTTCTGTGATGCTGACAGCCAGAAGAACTGGCTGGACCCCTCCAAGGAGATCAAGAAGCAGATCCGGA
  GTAGCCCCTGGAATTTTGCCTTCACAGTCAAGTTCTACCCGCCTGATCCTGCCCAGCTGACAGAAGACAT
  CACAAGATACTACCTGTGCCTGCAGCTGCGGGCAGACATCATCACGGGCCGGCTGCCATGCTCCTTTGTC
  ACGCATGCCCTACTGGGCTCCTACGCTGTGCAGGCTGAGCTGGGTGACTATGATGCTGAGGAGCATGTGG
  GCAACTATGTCAGCGAGCTCCGCTTCGCCCCTAACCAGACCCGGGAGCTGGAGGAGAGGATCATGGAGCT
  GCATAAGACATATAGGGGGATGACCCCGGGAGAAGCAGAAATCCACTTCTTAGAGAATGCCAAGAAGCTT
  TCCATGTACGGAGTAGACCTGCACCATGCCAAGGACTCTGAGGGCATCGACATCATGTTAGGCGTTTGTG
  CCAATGGCCTGCTCATCTACCGGGACCGGCTGAGAATCAACCGCTTTGCCTGGCCCAAGATCCTCAAGAT
  CTCCTACAAGAGGAGTAACTTCTATATCAAGATCCGGCCTGGGGAGTATGAGCAATTTGAGAGCACAATT
  GGCTTTAAGCTCCCAAACCACCGGTCAGCCAAGAGACTGTGGAAGGTCTGCATCGAGCATCATACATTCT
  TCCGGCTGGTGTCCCCTGAGCCCCCACCCAAGGGCTTCCTGGTGATGGGCTCCAAGTTCCGGTACAGTGG
  GAGGACCCAGGCACAGACTCGCCAGGCCAGCGCCCTCATTGACCGGCCTGCACCCTTCTTTGAGCGTTCT
  TCCAGCAAACGGTACACCATGTCCCGCAGCCTTGATGGAGCAGAGTTCTCCCGCCCAGCCTCGGTCAGCG
  AGAACCATGATGCAGGGCCTGACGGTGACAAGCGGGATGAGGATGGCGAGTCTGGGGGGCAACGGTCAGA
  GGCTGAGGAGGGAGAGGTCAGGACTCCAACCAAGATCAAGGAGCTAAAGCCGGAGCAGGAAACCACGCCG
  AGACACAAGCAGGAGTTCTTAGACAAGCCAGAAGATGTCTTGCTGAAGCACCAGGCCAGCATCAATGAGC
  TCAAAAGGACCCTGAAGGAGCCCAACAGCAAACTCATCCACCGGGATCGAGACTGGGAACGGGAGCGCAG
  GCTGCCCTCCTCCCCCGCCTCCCCCTCCCCCAAGGGCACCCCTGAGAAAGCCAATGAGAGAGCAGGGCTG
  AGGGAGGGCTCCGAGGAGAAAGTCAAACCACCACGTCCCCGGGCCCCAGAGAGTGACACAGGCGATGAGG
  ACCAGGACCAGGAGAGGGACACGGTGTTCCTGAAGGACAACCACCTGGCCATTGAGCGCAAGTGCTCCAG
  CATCACGGTCAGCTCTACGTCTAGCCTGGAGGCTGAGGTGGACTTCACGGTCATTGGTGACTACCATGGC
  AGCGCCTTCGAAGACTTCTCCCGCAGCCTGCCTGAGCTCGACCGGGACAAAAGCGACTCGGACACTGAGG
  GCCTGCTGTTCTCCCGGGATCTCAACAAGGGGGCCCCCAGCCAGGATGATGAGTCTGGGGGCATTGAGGA
  CAGCCCGGATCGAGGGGCCTGCTCCACCCCGGATATGCCCCAGTTTGAGCCCGTGAAAACAGAAACCATG
  ACTGTCAGCAGTCTGGCCATTAGAAAGAAGATTGAGCCGGAGGCCGTACTGCAGACCAGAGTCTCCGCTA
  TGGATAACACCCAGGTTGATGGGAGTGCCTCAGTGGGGAGGGAGTTCATAGCAACCACTCCCTCCATCAC
  CACGGAGACCATATCGACCACCATGGAGAACAGTCTCAAGTCCGGGAAGGGGGCAGCTGCCATGATCCCA
  GGCCCACAGACGGTGGCCACGGAAATCCGTTCTCTTTCTCCGATCATCGGGAAAGATGTCCTCACCAGCA
  CCTACGGCGCCACTGCGGAAACCCTCTCAACCTCCACCACCACCCATGTCACCAAAACTGTGAAAGGAGG
  GTTTTCTGAGACAAGGATCGAGAAGCGAATCATCATTACTGGGGATGAAGATGTCGATCAAGACCAGGCC
  CTGGCTTTGGCCATCAAGGAGGCCAAACTGCAGCATCCTGATATGCTGGTAACCAAAGCTGTCGTATACA
  GAGAAACAGACCCATCCCCAGAGGAGAGGGACAAGAAGCCACAGGAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234991 representing NM_001258329
  Red=Cloning site Green=Tags(s)
  
  MTTETGPDSEVKKAQEEAPQQPEAAAAVTTPVTPAGHGHPEANSNEKHPSQQDTRPAEQSLDMEEKDYSE
  ADGLSERTTPSKAQKSPQKIAKKYKSAICRVTLLDASEYECEVEKHGRGQVLFDLVCEHLNLLEKDYFGL
  TFCDADSQKNWLDPSKEIKKQIRSSPWNFAFTVKFYPPDPAQLTEDITRYYLCLQLRADIITGRLPCSFV
  THALLGSYAVQAELGDYDAEEHVGNYVSELRFAPNQTRELEERIMELHKTYRGMTPGEAEIHFLENAKKL
  SMYGVDLHHAKDSEGIDIMLGVCANGLLIYRDRLRINRFAWPKILKISYKRSNFYIKIRPGEYEQFESTI
  GFKLPNHRSAKRLWKVCIEHHTFFRLVSPEPPPKGFLVMGSKFRYSGRTQAQTRQASALIDRPAPFFERS
  SSKRYTMSRSLDGAEFSRPASVSENHDAGPDGDKRDEDGESGGQRSEAEEGEVRTPTKIKELKPEQETTP
  RHKQEFLDKPEDVLLKHQASINELKRTLKEPNSKLIHRDRDWERERRLPSSPASPSPKGTPEKANERAGL
  REGSEEKVKPPRPRAPESDTGDEDQDQERDTVFLKDNHLAIERKCSSITVSSTSSLEAEVDFTVIGDYHG
  SAFEDFSRSLPELDRDKSDSDTEGLLFSRDLNKGAPSQDDESGGIEDSPDRGACSTPDMPQFEPVKTETM
  TVSSLAIRKKIEPEAVLQTRVSAMDNTQVDGSASVGREFIATTPSITTETISTTMENSLKSGKGAAAMIP
  GPQTVATEIRSLSPIIGKDVLTSTYGATAETLSTSTTTHVTKTVKGGFSETRIEKRIIITGDEDVDQDQA
  LALAIKEAKLQHPDMLVTKAVVYRETDPSPEERDKKPQES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258329
• ORF Size: 2640 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258329.1, NP_001245258.1
• RefSeq Size: 6327 bp
• RefSeq ORF: 2643 bp
• Locus ID: 2036
• Cytogenetics: 20q11.23
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• Gene Summary: 'Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]'