MTHFD1L (NM_001242767) Human Tagged ORF Clone

CAT#: RG235098

MTHFD1L (GFP-tagged) - Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MTHFD1L
• Synonyms: dJ292B18.2; FTHFSDC1; MTC1THFS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235098 representing NM_001242767
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACGCGTCTGCCGCTCGTCCTGCGCCAGCTCCGCCGCCCGCCCCAGCCCCCGGGCCCTCCGCGCC
  GCCTCCGTGTGCCCTGTCGCGCTAGCAGCGGCGGCGGCGGAGGCGGCGGCGGTGGCCGGGAGGGCCTGCT
  TGGACAGCGGCGGCCGCAGGATGGCCAGGCCCGGAGCAGCTGCAGCCCCGGCGGCCGAACGCCCGCGGCG
  CGGGACTCCATCGTCAGAGAAGTCATTCAGAATTCAAAAGAAGTTCTAAGTTTATTGCAAGAAAAAAACC
  CTGCCTTCAAGCCGGTTCTTGCAATTATCCAGGCAGGTGACGACAACTTGATGCAGGAAATCAACCAGAA
  TTTGGCTGAGGAGGCTGGTCTGAACATCACTCACATTTGCCTCCCTCCAGATAGCAGTGAAGCCGAGATT
  ATAGATGAAATCTTAAAGATCAATGAAGATACCAGAGTACATGGCCTTGCCCTTCAGATCTCTGAGAACT
  TGTTTAGCAACAAAGTCCTCAATGCCTTGAAACCAGAAAAAGATGTGGATGGAGTAACAGACATAAACCT
  GGGGAAGCTGGTGCGAGGGGATGCCCATGAATGTTTTGTTTCACCTGTTGCCAAAGCTGTAATTGAACTT
  CTTGAAAAATCAGTAGGTGTCAACCTAGATGGAAAGAAGATTTTGGTAGTGGGGGCCCATGGGTCTTTGG
  AAGCTGCTCTACAATGCCTGTTCCAGAGAAAAGGGTCCATGACAATGAGCATCCAGTGGAAAACACGCCA
  GCTTCAAAGCAAGCTTCACGAGGCTGACATTGTGGTCCTAGGCTCACCTAAGCCAGAAGAGATTCCCCTT
  ACTTGGATACAACCAGGAACTACTGTTCTCAACTGCTCCCATGACTTCCTGTCAGGGAAGGTTGGGTGTG
  GCTCTCCAAGAATACATTTTGGTGGACTCATTGAGGAAGATGATGTGATTCTCCTTGCTGCAGCTCTGCG
  AATTCAGAACATGGTCAGTAGTGGAAGGAGATGGCTTCGTGAACAGCAGCACAGGCGGTGGAGACTTCAC
  TGCTTGAAACTTCAGCCTCTCTCCCCTGTGCCAAGTGACATTGAGATTTCAAGAGGACAAACTCCAAAAG
  CTGTGGATGTCCTTGCCAAGGAGATTGGATTGCTTGCAGATGAAATTGAAATCTATGGCAAAAGCAAAGC
  CAAAGTACGTTTGTCCGTGCTAGAAAGGTTAAAGGATCAAGCAGATGGAAAATACGTCTTAGTTGCTGGG
  ATCACACCCACCCCTCTTGGAGAAGGGAAGAGCACAGTCACCATCGGGCTTGTGCAGGCTCTGACCGCAC
  ACCTGAATGTCAACTCCTTTGCCTGCTTGAGGCAGCCTTCCCAAGGACCGACGTTTGGAGTGAAAGGAGG
  AGCCGCGGGTGGTGGATATGCCCAGGTCATCCCCATGGAGGAGTTCAACCTTCACTTGACTGGAGACATC
  CACGCCATCACCGCTGCCAATAACTTGCTGGCTGCCGCCATCGACACGAGGATTCTTCATGAAAACACGC
  AAACAGATAAGGCTCTGTATAATCGGCTGGTTCCTTTAGTGAATGGTGTCAGAGAATTTTCAGAAATTCA
  GCTTGCTCGGCTAAAAAAACTGGGAATAAATAAGACTGATCCGAGCACACTGACAGAAGAGGAAGTGAGT
  AAATTTGCCCGTCTCGACATCGACCCATCTACCATCACGTGGCAGAGAGTATTGGATACAAATGACCGAT
  TTCTACGAAAAATAACCATCGGGCAGGGAAACACAGAGAAGGGCCATTACCGGCAGGCGCAGTTTGACAT
  CGCAGTGGCCAGCGAGATCATGGCGGTGCTGGCCCTGACGGACAGCCTCGCAGACATGAAGGCACGGCTG
  GGAAGGATGGTGGTGGCCAGTGACAAAAGCGGGCAGCCTGTGACAGCAGATGATTTGGGGGTGACAGGTG
  CTTTGACAGTTTTGATGAAAGATGCAATAAAACCAAACCTGATGCAGACCCTGGAAGGGACACCTGTGTT
  CGTGCATGCGGGCCCTTTTGCTAACATTGCTCACGGCAACTCTTCAGTGTTGGCTGATAAAATTGCCCTG
  AAACTGGTTGGTGAAGAAGGATTTGTAGTGACCGAAGCTGGCTTTGGTGCTGACATCGGAATGGAGAAAT
  TCTTCAACATCAAGTGCCGAGCTTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGCAACGGTGCGAGC
  TCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAGAAAGAATATACAGAGGAG
  AACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTCAGCTCTTTGGGG
  TTCCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTTGGTGTGTGAGCT
  TGCAAAGCGGGCTGGTGCCTTTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGAAAAGGATCGGTG
  GACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGTATGATGTTCAGG
  TTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATATTGAACTCTCTCC
  TGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATCTGCATGGCAAAG
  ACCCACCTTTCTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCTTACCTATCAGTG
  ACGTCCGGGCCAGCATAGGCGCTGGGTTCATTTACCCTTTGGTCGGAACGATGAGCACCATGCCAGGACT
  GCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAAGTTAAAGGCTTGTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235098 representing NM_001242767
  Red=Cloning site Green=Tags(s)
  
  MGTRLPLVLRQLRRPPQPPGPPRRLRVPCRASSGGGGGGGGGREGLLGQRRPQDGQARSSCSPGGRTPAA
  RDSIVREVIQNSKEVLSLLQEKNPAFKPVLAIIQAGDDNLMQEINQNLAEEAGLNITHICLPPDSSEAEI
  IDEILKINEDTRVHGLALQISENLFSNKVLNALKPEKDVDGVTDINLGKLVRGDAHECFVSPVAKAVIEL
  LEKSVGVNLDGKKILVVGAHGSLEAALQCLFQRKGSMTMSIQWKTRQLQSKLHEADIVVLGSPKPEEIPL
  TWIQPGTTVLNCSHDFLSGKVGCGSPRIHFGGLIEEDDVILLAAALRIQNMVSSGRRWLREQQHRRWRLH
  CLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVRLSVLERLKDQADGKYVLVAG
  ITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHLTGDI
  HAITAANNLLAAAIDTRILHENTQTDKALYNRLVPLVNGVREFSEIQLARLKKLGINKTDPSTLTEEEVS
  KFARLDIDPSTITWQRVLDTNDRFLRKITIGQGNTEKGHYRQAQFDIAVASEIMAVLALTDSLADMKARL
  GRMVVASDKSGQPVTADDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLADKIAL
  KLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKEYTEE
  NIQLVADGCCNLQKQIQITQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSV
  DLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQGFGNLPICMAK
  THLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQVKGLF
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242767
• ORF Size: 2937 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001242767.1, NP_001229696.1
• RefSeq Size: 3490
• RefSeq ORF: 2940
• Locus ID: 25902
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate
• Gene Summary: The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]