GLT28D1 (ALG13) (NM_001257231) Human Tagged ORF Clone

CAT#: RG235144

ALG13 (GFP-tagged) - Homo sapiens ALG13, UDP-N-acetylglucosaminyltransferase subunit (ALG13), transcript variant 7

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ALG13
• Synonyms: CDG1S; CXorf45; EIEE36; GLT28D1; MDS031; TDRD13; YGL047W
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235144 representing NM_001257231
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTACAGGTGCAGGAAGCTGTTTGGAGACTCTGGAAAAAGGAAAGCCACTCGTAGTGGTTATAAACG
  AAAAGTTGATGAACAATCATCAGCTGGAACTGGCAAAGCAGCTACACAAAGAGGGTCATCTCTTCTATTG
  TACCTGCAGGGTCCTGACTTGTCCTGGGCAAGCCAAGTCCATTGCTTCTGCTCCTGGGAAGTGCCAAGAT
  TCTGCAGCGCTGACTTCAACTGCCTTTTCAGGCCTAGACTTTGGGCTGCTTTCCGGATACCTGCATAAGC
  AAGCCCTTGTTACTGCTACCCATCCTACCTGCACCCTGCTTTTTCCCTCTTGCCACGCTTTTTTTCCTCT
  CCCTCTTACCCCCACCCTGTACAAAATGCATAAAGGATGGAAAAACTACTGCAGCCAGAAGTCTTTGAAT
  GAGGCATCAATGGATGAATATTTAGGCAGCTTAGGGCTGTTTCGAAAGCTGACTGCCAAGGATGCCTCTT
  GCCTCTTTCGGGCCATTTCGGAGCAGTTGTTTTGCAGCCAGGTCCATCATTTGGAAATCAGGAAGGCTTG
  TGTCTCATATATGAGGGAAAATCAACAAACTTTTGAGTCTTATGTGGAGGGATCTTTTGAGAAATACCTG
  GAACGGTTGGGAGATCCCAAGGAAAGTGCTGGCCAGCTGGAAATAAGAGCTCTTTCTCTAATTTATAATC
  GGGATTTCATTCTTTATCGCTTTCCTGGAAAACCTCCAACTTATGTCACAGATAATGGCTATGAAGACAA
  GATTCTACTCTGCTACTCAAGTAGTGGTCACTATGATTCTGTGTACTCAAAACAATTTCAGTCAAGTGCA
  GCTGTTTGTCAGGCTGTATTGTACGAAATTCTCTATAAAGATGTGTTTGTTGTGGATGAAGAAGAGTTGA
  AGACTGCGATTAAATTGTTTCGAAGTGGTTCTAAGAAGAACAGAAATAATGCTGTAACTGGAAGCGAGGA
  TGCCCATACTGATTACAAGAGTTCAAATCAGAATAGGATGGAAGAGTGGGGTGCCTGCTACAATGCTGAA
  AATATACCAGAGGGCTACAATAAAGGAACAGAAGAAACAAAGTCTCCAGAAAATCCATCAAAGATGCCCT
  TCCCCTATAAGGTGCTCAAAGCCCTGGATCCAGAAATCTATCGTAATGTAGAATTTGATGTTTGGTTGGA
  CAGCAGAAAAGAACTTCAAAAATCTGATTACATGGAGTATGCTGGGAGACAGTACTATTTGGGAGACAAG
  TGTCAGGTTTGCTTGGAATCAGAAGGAAGATATTATAATGCTCATATCCAGGAAGTTGGAAATGAGAACA
  ATTCAGTAACAGTCTTCATTGAAGAATTGGCGGAAAAGCATGTTGTTCCACTGGCTAACTTAAAACCAGT
  TACCCAAGTGATGTCTGTTCCTGCCTGGAATGCTATGCCCAGTCGGAAAGGAAGAGGTTACCAGAAAATG
  CCTGGGGGTTATGTCCCGGAAATAGTTATATCAGAGATGGACATAAAGCAACAGAAGAAGATGTTCAAGA
  AAATTCGAGGGAAAGAAGTTTACATGACTATGGCTTACGGCAAGGGAGACCCCCTCCTCCCACCCAGGCT
  GCAGCACAGTATGCATTATGGGCACGATCCTCCAATGCACTACTCACAGACAGCTGGCAATGTTATGTCT
  AATGAACATTTTCATCCTCAGCATCCATCTCCGAGACAAGGTCGGGGATATGGGATGCCCAGGAATTCAT
  CTCGGTTTATAAACAGGCACAACATGCCGGGCCCTAAAGTTGATTTTTACCCAGGCCCAGGTAAAAGGTG
  CTGCCAGAGCTATGATAACTTCTCTTATAGATCTCGTTCATTTAGACGTAGTCACCGCCAGATGAGTTGT
  GTGAATAAGGAGTCCCAGTATGGATTTACCCCAGGGAATGGACAGATGCCCAGGGGCTTGGAAGAAACTA
  TTACTTTTTATGAAGTTGAAGAAGGGGATGAGACTGCTTATCCAACTTTACCTAATCATGGAGGTCCCTC
  TACAATGGTTCCTGCTACTTCAGGATACTGTGTTGGAAGGCGGGGACATAGCTCAGGCAAACAGACTTTG
  AATTTAGAGGAGGGCAATGGCCAGAGTGAAAATGGGCGATATCATGAAGAATATCTTTATCGTGCAGAGC
  CAGACTATGAAACTTCAGGTGTTTATAGCACAACTGCATCTACAGCAAACTTGTCTCTTCAGGACAGAAA
  GTCATGTTCTATGTCTCCTCAGGACACAGTTACCTCATACAACTACCCCCAGAAGATGATGGGAAATATT
  GCAGCAGTTGCAGCTTCCTGTGCCAATAATGTTCCAGCTCCAGTCTTATCTAACGGTGCAGCGGCTAATC
  AAGCTATTAGTACCACTTCAGTTTCCTCACAGAATGCTATACAGCCTCTCTTTGTATCTCCACCTACACA
  CGGCAGGCCAGTGATTGCCTCACCATCCTATCCATGCCATTCTGCTATTCCTCATGCTGGTGCCTCTCTA
  CCACCACCACCACCACCACCACCACCACCACCACCACCACCACCTCCTCCTCCTCCTCCTCCTCCTCCTC
  CTCCTCCTCCTGCTCTTGATGTGGGAGAGACTTCAAACTTACAACCACCACCACCACTACCACCTCCACC
  TTATTCCTGTGATCCAAGCGGCAGTGATTTGCCTCAAGATACAAAAGTTTTGCAGTACTATTTCAATCTA
  GGATTGCAGTGCTATTACCACAGCTACTGGCACTCCATGGTCTATGTGCCACAGATGCAGCAGCAGCTTC
  ATGTAGAGAATTATCCAGTCTATACTGAGCCACCTCTGGTAGATCAAACCGTTCCTCAATGCTACAGTGA
  GGTGAGGAGAGAAGATGGCATACAGGCGGAAGCATCAGCAAATGATACTTTTCCGAATGCTGATTCTTCA
  TCTGTCCCTCATGGAGCAGTCTATTATCCAGTAATGTCAGATCCCTATGGGCAGCCACCTTTGCCAGGTT
  TTGACTCCTGCCTTCCGGTTGTGCCAGATTATTCCTGTGTTCCCCCCTGGCATCCAGTTGGTACAGCATA
  TGGTGGTTCTTCTCAAATTCATGGTGCTATAAATCCTGGGCCAATTGGCTGTATTGCTCCATCTCCCCCA
  GCTTCTCATTATGTACCTCAGGGTATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235144 representing NM_001257231
  Red=Cloning site Green=Tags(s)
  
  MFTGAGSCLETLEKGKPLVVVINEKLMNNHQLELAKQLHKEGHLFYCTCRVLTCPGQAKSIASAPGKCQD
  SAALTSTAFSGLDFGLLSGYLHKQALVTATHPTCTLLFPSCHAFFPLPLTPTLYKMHKGWKNYCSQKSLN
  EASMDEYLGSLGLFRKLTAKDASCLFRAISEQLFCSQVHHLEIRKACVSYMRENQQTFESYVEGSFEKYL
  ERLGDPKESAGQLEIRALSLIYNRDFILYRFPGKPPTYVTDNGYEDKILLCYSSSGHYDSVYSKQFQSSA
  AVCQAVLYEILYKDVFVVDEEELKTAIKLFRSGSKKNRNNAVTGSEDAHTDYKSSNQNRMEEWGACYNAE
  NIPEGYNKGTEETKSPENPSKMPFPYKVLKALDPEIYRNVEFDVWLDSRKELQKSDYMEYAGRQYYLGDK
  CQVCLESEGRYYNAHIQEVGNENNSVTVFIEELAEKHVVPLANLKPVTQVMSVPAWNAMPSRKGRGYQKM
  PGGYVPEIVISEMDIKQQKKMFKKIRGKEVYMTMAYGKGDPLLPPRLQHSMHYGHDPPMHYSQTAGNVMS
  NEHFHPQHPSPRQGRGYGMPRNSSRFINRHNMPGPKVDFYPGPGKRCCQSYDNFSYRSRSFRRSHRQMSC
  VNKESQYGFTPGNGQMPRGLEETITFYEVEEGDETAYPTLPNHGGPSTMVPATSGYCVGRRGHSSGKQTL
  NLEEGNGQSENGRYHEEYLYRAEPDYETSGVYSTTASTANLSLQDRKSCSMSPQDTVTSYNYPQKMMGNI
  AAVAASCANNVPAPVLSNGAAANQAISTTSVSSQNAIQPLFVSPPTHGRPVIASPSYPCHSAIPHAGASL
  PPPPPPPPPPPPPPPPPPPPPPPPPPPALDVGETSNLQPPPPLPPPPYSCDPSGSDLPQDTKVLQYYFNL
  GLQCYYHSYWHSMVYVPQMQQQLHVENYPVYTEPPLVDQTVPQCYSEVRREDGIQAEASANDTFPNADSS
  SVPHGAVYYPVMSDPYGQPPLPGFDSCLPVVPDYSCVPPWHPVGTAYGGSSQIHGAINPGPIGCIAPSPP
  ASHYVPQGM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001257231
• ORF Size: 3177 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001257231.1, NP_001244160.1
• RefSeq Size: 4193
• RefSeq ORF: 3180
• Locus ID: 79868
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]