DIAPH3 (NM_001258367) Human Tagged ORF Clone

CAT#: RG235187

DIAPH3 (GFP-tagged) - Homo sapiens diaphanous-related formin 3 (DIAPH3), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DIAPH3
• Synonyms: AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235187 representing NM_001258367
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGGCACCAGCCGCGGCTGCACCACCCGGCCCAAGGCTCAGCCGCTGGGACTCCCTACCCTTCCT
  CAGCCTCTCTCCGCGGCTGCCGGGAAAGCAAGATGCCGCGCAGGAAGGGCCCCCAACACCCTCCGCCGCC
  CAGTGGCCCCGAGGAGCCTGGGGAGAAGCGCCCCAAGTTTCTGGACAAATTTGCCAGCATAAGAATTCCA
  GGGAGCAAGAAAGAGAGACCTCCACTTCCCAACCTGAAGACTGCATTTGCAAGCAGTGATTGCTCAGCAG
  CACCTTTAGAGATGATGGAGAACTTTCCAAAGCCACTGTCAGAGAATGAACTCTTAGAACTTTTTGAAAA
  AATGATGGGAAGTCTTAAGAGAAGCCGACAGATCTCACCTCAGGAATTCATTCATGAGCTGAAAATGGGG
  TCTGCAGATGAGAGACTTGTCACATGCCTGGAGTCTCTCCGAGTGTCTTTGACCAGCAATCCTGTGAGTT
  GGGTGGAAAGCTTTGGACATGAAGGGCTTGGATTATTATTAGACATTTTGGAAAAACTGATTAGTGGAAA
  AATCCAAGAAAAAGTTGTAAAGAAAAATCAACATAAAGTCATACAGTGTCTAAAAGCCCTGATGAATACG
  CAGTATGGCTTGGAAAGAATTATGAGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCA
  GACACCCCAATATGATGACAGATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCAT
  CCTTGAAGAAGTTTTAGAAGCTTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATT
  GTGGAAGGCCTCCGGCACAATTCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTA
  CATCTCCTGATGATTTGGATTTCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGAT
  ATTGCCAAATTTAAAATGCATTAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAA
  GAAGAAGATTTGTTTGAGTTATCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATG
  TTTACAACATGGTGTGGAGCACAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCA
  TCTTTTGCTGATTCGAAATGATTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCC
  CAGATTGTATTGCATAGAGATGGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAA
  CCCAGTTTGTAGACATTTGCATAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTA
  CAAGAAATTTGAAAAAGAGTTTACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAG
  ATTAATGAGCTTCAAGCAGAGCTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATA
  TTCCTTTGCCTCCCTCTAAAGAAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTC
  TGGTGGAGGGGTGCCGCCTCCACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTC
  AGTGGTCCTGTGCCTCCACCACCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCC
  TGCCATTTGGGTTGAAACCAAAGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAA
  GATCAGACCTCATGAAATGACTGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTG
  GATTTGCTTTGTAAACTTGAGAATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTG
  AAGAGAAGAAATCGATTAAGAAAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCT
  TTCAATCTTCCTGAGCTCTTTTCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAA
  ACACGGTTGGCAGAGTCTATGATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCAT
  TGTCTCAGTTCAAGAGTGAATATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAATGT
  GAAGAGACTACGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATC
  AAACCTGACATCATGGCTGTCAGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTGC
  TGGAACTTGTATTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTAA
  CCTTAGCTCTCTCTGTAAACTAAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCATTTCCTG
  GTAGAAATATGTGAAGAGAAGTACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTAGACAAAG
  CTAGTAAAGTCTCTGTAGAAACGCTGGAAAAGAATTTGAGGCAGATGGGAAGGCAGCTTCAACAGCTTGA
  GAAGGAATTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGACAAAGATGTCCAGATTT
  GTTATCAGTGCAAAAGAACAATATGAGACACTTTCGAAGTTACACGAAAACATGGAAAAGTTATACCAGA
  GTATAATAGGATACTATGCCATTGATGTGAAGAAGGTGTCTGTGGAAGACTTTCTTACTGACCTGAATAA
  CTTCAGAACCACATTCATGCAAGCAATAAAGGAGAATATCAAAAAAAGAGAAGCAGAGGAAAAAGAAAAA
  CGTGTCAGAATAGCTAAAGAATTAGCAGAGCGAGAAAGACTCGAACGCCAACAAAAGAAAAAGCGTTTAT
  TAGAAATGAAGACTGAGGGTGATGAGACAGGAGTGATGGATAATCTGCTGGAGGCCTTGCAGTCCGGGGC
  TGCCTTCCGCGACAGAAGAAAAAGGACACCGATGCCAAAAGATGTTCGGCAGAGTCTCAGTCCAATGTCT
  CAGAGGCCTGTTCTGAAAGTTTGTAACCATGAAAATCAGAAAGTGCAGTTGACAGAAGGGTCACGTTCAC
  ACTACAATATCAATTGCAACTCAACAAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGACACTCA
  TACGTCTACTGGGAGGATCAAGGCAGCTGAGAAGAAGGAAGCGTGTAATGTAGAAAGCAACAGAAAAAAG
  GAAACGGAACTTCTTGGCTCTTTTTCTAAAAATGAATCAGTTCCCGAAGTTGAAGCCCTGCTGGCAAGAT
  TACGAGCTTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235187 representing NM_001258367
  Red=Cloning site Green=Tags(s)
  
  MERHQPRLHHPAQGSAAGTPYPSSASLRGCRESKMPRRKGPQHPPPPSGPEEPGEKRPKFLDKFASIRIP
  GSKKERPPLPNLKTAFASSDCSAAPLEMMENFPKPLSENELLELFEKMMGSLKRSRQISPQEFIHELKMG
  SADERLVTCLESLRVSLTSNPVSWVESFGHEGLGLLLDILEKLISGKIQEKVVKKNQHKVIQCLKALMNT
  QYGLERIMSEERSLSLLAKAVDPRHPNMMTDVVKLLSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCI
  VEGLRHNSVQLQVACMQLINALVTSPDDLDFRLHIRNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHK
  EEDLFELSHRLEDIRAELDEAYDVYNMVWSTVKETRAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVS
  QIVLHRDGMDPDFTYRKRLDLDLTQFVDICIDQAKLEEFEEKASELYKKFEKEFTDHQETQAELQKKEAK
  INELQAELQAFKSQFGALPADCNIPLPPSKEGGTGHSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPF
  SGPVPPPPPLGFLGGQNSPPLPILPFGLKPKKEFKPEISMRRLNWLKIRPHEMTENCFWIKVNENKYENV
  DLLCKLENTFCCQQKERREEEDIEEKKSIKKKIKELKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDE
  TRLAESMIQNLIKHLPDQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNI
  KPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFL
  VEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRF
  VISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEK
  RVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMS
  QRPVLKVCNHENQKVQLTEGSRSHYNINCNSTRTPVAKELNYNLDTHTSTGRIKAAEKKEACNVESNRKK
  ETELLGSFSKNESVPEVEALLARLRAL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258367
• ORF Size: 3441 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001258367.1, NP_001245296.1
• RefSeq Size: 4674
• RefSeq ORF: 3444
• Locus ID: 81624
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]