RFC1 (NM_001204747) Human Tagged ORF Clone

CAT#: RG235188

RFC1 (GFP-tagged) - Homo sapiens replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RFC1
• Synonyms: A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235188 representing NM_001204747
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATTCGGAAATTCTTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGA
  ATGAGAAAACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAATAG
  CTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATCTATGATTCAGAT
  TCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCAGAAAAACTGCCAGTATCTTCTA
  AACCTGGTAAAATTTCACGGCAGGATCCTGTTACATACATTTCAGAAACAGATGAAGAAGATGACTTTAT
  GTGTAAGAAGGCGGCCTCTAAATCAAAAGAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAAC
  ATGAAAAAGAATGAAGAAAACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACAT
  CAGTACTTGATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGAAA
  AGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAATTACAGCTTGAT
  GAAGATGCGGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAGTTTGCCAGAACATTAGCCATGTTGGATG
  AAGAACCCAAGACCAAAAAGGCTCGAAAGGACACAGAAGCGGGAGAAACGTTTTCATCTGTCCAAGCCAA
  TTTAAGTAAAGCAGAAAAACATAAATATCCTCATAAAGTAAAAACAGCACAAGTTTCAGATGAAAGAAAG
  AGCTACAGTCCTAGGAAGCAAAGTAAATATGAAAGTTCAAAAGAATCTCAGCAACATTCCAAGTCATCAG
  CTGACAAAATAGGAGAAGTCTCTTCTCCCAAGGCCAGTTCTAAGCTGGCAATTATGAAAAGAAAAGAAGA
  GAGCTCTTATAAAGAAATAGAGCCTGTGGCCTCAAAAAGAAAAGAAAATGCCATTAAATTGAAAGGAGAG
  ACAAAAACTCCTAAGAAAACCAAAAGTTCTCCAGCTAAAAAAGAGTCTGTAAGTCCTGAAGATTCTGAAA
  AGAAACGCACTAATTATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCCAAGGCTCTGGGCTCCAA
  AGAAATACCAAAGGGAGCTGAAAATTGCTTGGAAGGCCTTATATTTGTAATCACAGGCGTGCTGGAGTCT
  ATTGAACGAGATGAGGCCAAGTCTCTAATTGAACGTTATGGGGGAAAAGTAACAGGAAATGTCAGCAAGA
  AAACAAATTATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGAC
  AAAAATTATTGATGAAGATGGCCTGTTGAATCTGATTCGGACTATGCCAGGCAAGAAATCCAAGTATGAA
  ATAGCAGTTGAAACTGAGATGAAGAAAGAGTCCAAACTGGAGAGAACACCCCAAAAAAATGTCCAAGGAA
  AAAGAAAAATTAGTCCATCTAAAAAGGAATCAGAATCTAAAAAGAGCAGGCCGACTTCCAAAAGGGACAG
  TTTGGCAAAGACAATAAAAAAGGAAACAGATGTGTTTTGGAAAAGCCTGGATTTCAAGGAGCAGGTGGCT
  GAGGAGACAAGTGGTGACAGCAAGGCTAGGAATTTGGCTGATGACAGCAGTGAAAACAAAGTGGAAAATT
  TGCTCTGGGTGGATAAATATAAGCCAACCTCGCTCAAGACCATAATTGGACAGCAAGGTGACCAGAGCTG
  TGCCAACAAACTCCTACGCTGGCTCCGAAACTGGCAAAAGAGTTCTTCCGAAGATAAAAAACACGCAGCA
  AAGTTTGGTAAATTTTCCGGCAAAGATGATGGCTCTAGTTTTAAAGCAGCGTTGCTGTCAGGCCCTCCTG
  GTGTTGGCAAAACCACCACAGCTTCCCTGGTGTGTCAGGAGTTGGGATACAGCTACGTGGAACTGAATGC
  AAGTGACACCCGGAGTAAGAGCAGTTTGAAGGCGATTGTTGCTGAGTCACTGAACAATACCAGCATCAAA
  GGCTTTTATTCAAATGGAGCAGCCTCTTCAGTAAGCACGAAACATGCTCTCATCATGGATGAAGTAGATG
  GCATGGCAGGCAATGAGGATAGGGGAGGAATTCAGGAATTAATTGGCCTGATAAAACATACTAAAATTCC
  CATTATTTGTATGTGCAATGATAGAAATCATCCCAAGATTCGCTCTCTGGTTCATTATTGTTTTGATCTT
  CGTTTTCAAAGACCTCGGGTTGAACAGATTAAGGGTGCTATGATGTCTATTGCATTTAAAGAAGGTTTAA
  AGATTCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCCAATCAAGATATCAGACAGGTTTTACATAA
  TCTGAGTATGTGGTGTGCACGAAGTAAAGCATTAACCTATGACCAGGCCAAAGCTGATTCTCACAGAGCC
  AAAAAGGATATCAAAATGGGCCCATTTGATGTTGCCCGGAAAGTGTTTGCAGCTGGAGAGGAGACTGCTC
  ACATGTCACTTGTGGACAAGTCAGATCTCTTTTTTCATGATTATTCAATAGCACCCCTCTTCGTCCAGGA
  AAATTACATACACGTGAAGCCTGTAGCAGCAGGGGGTGACATGAAAAAGCACCTGATGCTTTTAAGCAGA
  GCAGCAGACAGCATATGCGATGGTGACCTAGTGGACAGCCAGATCCGGAGTAAGCAAAACTGGAGTCTTC
  TGCCTGCGCAGGCCATTTATGCCAGTGTTCTTCCTGGAGAGTTGATGAGGGGGTACATGACCCAGTTTCC
  CACCTTCCCAAGCTGGCTGGGGAAGCACTCGTCTACAGGCAAACATGATCGTATTGTTCAGGACCTGGCC
  TTGCATATGAGTCTCAGAACTTACTCCAGCAAAAGGACTGTAAACATGGATTATCTGTCGCTTCTAAGGG
  ATGCACTTGTACAGCCCTTGACCTCACAAGGAGTAGACGGAGTACAGGATGTTGTTGCACTTATGGACAC
  ATATTATTTGATGAAAGAAGACTTTGAGAATATCATGGAAATCAGCAGCTGGGGTGGCAAACCTAGTCCC
  TTTTCAAAGCTGGATCCCAAGGTGAAAGCAGCCTTCACAAGAGCTTACAATAAGGAAGCCCACCTTACTC
  CATACTCACTTCAAGCTATAAAGGCATCTAGACACAGCACAAGCCCATCCCTGGATTCGGAATACAATGA
  AGAATTAAATGAAGATGACTCTCAATCTGATGAGAAAGACCAAGATGCTATAGAAACTGATGCCATGATC
  AAGAAAAAGACAAAATCTTCAAAGCCTTCAAAACCAGAAAAAGATAAGGAGCCCAGAAAAGGAAAAGGAA
  AAAGTTCGAAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235188 representing NM_001204747
  Red=Cloning site Green=Tags(s)
  
  MDIRKFFGVIPSGKKLVSETVKKNEKTKSDEETLKAKKGIKEIKVNSSRKEDDFKQKQPSKKKRIIYDSD
  SESEETLQVKNAKKPPEKLPVSSKPGKISRQDPVTYISETDEEDDFMCKKAASKSKENGRSTNSHLGTSN
  MKKNEENTKTKNKPLSPIKLTPTSVLDYFGTGSVQRSNKKMVASKRKELSQNTDESGLNDEAIAKQLQLD
  EDAELERQLHEDEEFARTLAMLDEEPKTKKARKDTEAGETFSSVQANLSKAEKHKYPHKVKTAQVSDERK
  SYSPRKQSKYESSKESQQHSKSSADKIGEVSSPKASSKLAIMKRKEESSYKEIEPVASKRKENAIKLKGE
  TKTPKKTKSSPAKKESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLIFVITGVLES
  IERDEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKIIDEDGLLNLIRTMPGKKSKYE
  IAVETEMKKESKLERTPQKNVQGKRKISPSKKESESKKSRPTSKRDSLAKTIKKETDVFWKSLDFKEQVA
  EETSGDSKARNLADDSSENKVENLLWVDKYKPTSLKTIIGQQGDQSCANKLLRWLRNWQKSSSEDKKHAA
  KFGKFSGKDDGSSFKAALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKSSLKAIVAESLNNTSIK
  GFYSNGAASSVSTKHALIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDL
  RFQRPRVEQIKGAMMSIAFKEGLKIPPPAMNEIILGANQDIRQVLHNLSMWCARSKALTYDQAKADSHRA
  KKDIKMGPFDVARKVFAAGEETAHMSLVDKSDLFFHDYSIAPLFVQENYIHVKPVAAGGDMKKHLMLLSR
  AADSICDGDLVDSQIRSKQNWSLLPAQAIYASVLPGELMRGYMTQFPTFPSWLGKHSSTGKHDRIVQDLA
  LHMSLRTYSSKRTVNMDYLSLLRDALVQPLTSQGVDGVQDVVALMDTYYLMKEDFENIMEISSWGGKPSP
  FSKLDPKVKAAFTRAYNKEAHLTPYSLQAIKASRHSTSPSLDSEYNEELNEDDSQSDEKDQDAIETDAMI
  KKKTKSSKPSKPEKDKEPRKGKGKSSKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001204747
• ORF Size: 3444 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204747.1, NP_001191676.1
• RefSeq Size: 4899 bp
• RefSeq ORF: 3447 bp
• Locus ID: 5981
• Cytogenetics: 4p14
• Protein Families: Transcription Factors
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]'