RFC1 (NM_001204747) Human Untagged Clone

CAT#: SC331606

RFC1 (untagged) - Homo sapiens replication factor C (activator 1) 1, 145kDa (RFC1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFC1
• Synonyms: A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001204747, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACATTCGGAAATTCTTTGGAGTAATACCAAGTGGAAAGAAACTTGTAAGTGAAACAGTAAAGAAGA
  ATGAGAAAACAAAGTCTGATGAAGAAACTTTAAAAGCAAAGAAAGGAATAAAGGAAATCAAGGTAAATAG
  CTCCCGTAAAGAGGATGACTTCAAACAAAAGCAACCAAGCAAGAAAAAGAGGATCATCTATGATTCAGAT
  TCAGAGTCAGAGGAGACGTTGCAGGTAAAAAATGCCAAAAAGCCACCAGAAAAACTGCCAGTATCTTCTA
  AACCTGGTAAAATTTCACGGCAGGATCCTGTTACATACATTTCAGAAACAGATGAAGAAGATGACTTTAT
  GTGTAAGAAGGCGGCCTCTAAATCAAAAGAGAATGGAAGATCTACAAATAGTCATCTTGGAACATCAAAC
  ATGAAAAAGAATGAAGAAAACACTAAGACCAAGAATAAGCCTTTATCACCAATAAAACTTACACCCACAT
  CAGTACTTGATTATTTTGGAACTGGAAGTGTCCAAAGATCTAATAAGAAGATGGTGGCAAGCAAAAGAAA
  AGAGCTTTCACAAAATACAGATGAGTCTGGATTAAATGATGAAGCCATCGCCAAGCAATTACAGCTTGAT
  GAAGATGCGGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAGTTTGCCAGAACATTAGCCATGTTGGATG
  AAGAACCCAAGACCAAAAAGGCTCGAAAGGACACAGAAGCGGGAGAAACGTTTTCATCTGTCCAAGCCAA
  TTTAAGTAAAGCAGAAAAACATAAATATCCTCATAAAGTAAAAACAGCACAAGTTTCAGATGAAAGAAAG
  AGCTACAGTCCTAGGAAGCAAAGTAAATATGAAAGTTCAAAAGAATCTCAGCAACATTCCAAGTCATCAG
  CTGACAAAATAGGAGAAGTCTCTTCTCCCAAGGCCAGTTCTAAGCTGGCAATTATGAAAAGAAAAGAAGA
  GAGCTCTTATAAAGAAATAGAGCCTGTGGCCTCAAAAAGAAAAGAAAATGCCATTAAATTGAAAGGAGAG
  ACAAAAACTCCTAAGAAAACCAAAAGTTCTCCAGCTAAAAAAGAGTCTGTAAGTCCTGAAGATTCTGAAA
  AGAAACGCACTAATTATCAAGCTTATCGAAGCTACTTAAATCGAGAAGGTCCCAAGGCTCTGGGCTCCAA
  AGAAATACCAAAGGGAGCTGAAAATTGCTTGGAAGGCCTTATATTTGTAATCACAGGCGTGCTGGAGTCT
  ATTGAACGAGATGAGGCCAAGTCTCTAATTGAACGTTATGGGGGAAAAGTAACAGGAAATGTCAGCAAGA
  AAACAAATTATCTTGTCATGGGTCGTGATAGTGGACAGTCCAAGAGTGATAAGGCCGCAGCCTTGGGGAC
  AAAAATTATTGATGAAGATGGCCTGTTGAATCTGATTCGGACTATGCCAGGCAAGAAATCCAAGTATGAA
  ATAGCAGTTGAAACTGAGATGAAGAAAGAGTCCAAACTGGAGAGAACACCCCAAAAAAATGTCCAAGGAA
  AAAGAAAAATTAGTCCATCTAAAAAGGAATCAGAATCTAAAAAGAGCAGGCCGACTTCCAAAAGGGACAG
  TTTGGCAAAGACAATAAAAAAGGAAACAGATGTGTTTTGGAAAAGCCTGGATTTCAAGGAGCAGGTGGCT
  GAGGAGACAAGTGGTGACAGCAAGGCTAGGAATTTGGCTGATGACAGCAGTGAAAACAAAGTGGAAAATT
  TGCTCTGGGTGGATAAATATAAGCCAACCTCGCTCAAGACCATAATTGGACAGCAAGGTGACCAGAGCTG
  TGCCAACAAACTCCTACGCTGGCTCCGAAACTGGCAAAAGAGTTCTTCCGAAGATAAAAAACACGCAGCA
  AAGTTTGGTAAATTTTCCGGCAAAGATGATGGCTCTAGTTTTAAAGCAGCGTTGCTGTCAGGCCCTCCTG
  GTGTTGGCAAAACCACCACAGCTTCCCTGGTGTGTCAGGAGTTGGGATACAGCTACGTGGAACTGAATGC
  AAGTGACACCCGGAGTAAGAGCAGTTTGAAGGCGATTGTTGCTGAGTCACTGAACAATACCAGCATCAAA
  GGCTTTTATTCAAATGGAGCAGCCTCTTCAGTAAGCACGAAACATGCTCTCATCATGGATGAAGTAGATG
  GCATGGCAGGCAATGAGGATAGGGGAGGAATTCAGGAATTAATTGGCCTGATAAAACATACTAAAATTCC
  CATTATTTGTATGTGCAATGATAGAAATCATCCCAAGATTCGCTCTCTGGTTCATTATTGTTTTGATCTT
  CGTTTTCAAAGACCTCGGGTTGAACAGATTAAGGGTGCTATGATGTCTATTGCATTTAAAGAAGGTTTAA
  AGATTCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCCAATCAAGATATCAGACAGGTTTTACATAA
  TCTGAGTATGTGGTGTGCACGAAGTAAAGCATTAACCTATGACCAGGCCAAAGCTGATTCTCACAGAGCC
  AAAAAGGATATCAAAATGGGCCCATTTGATGTTGCCCGGAAAGTGTTTGCAGCTGGAGAGGAGACTGCTC
  ACATGTCACTTGTGGACAAGTCAGATCTCTTTTTTCATGATTATTCAATAGCACCCCTCTTCGTCCAGGA
  AAATTACATACACGTGAAGCCTGTAGCAGCAGGGGGTGACATGAAAAAGCACCTGATGCTTTTAAGCAGA
  GCAGCAGACAGCATATGCGATGGTGACCTAGTGGACAGCCAGATCCGGAGTAAGCAAAACTGGAGTCTTC
  TGCCTGCGCAGGCCATTTATGCCAGTGTTCTTCCTGGAGAGTTGATGAGGGGGTACATGACCCAGTTTCC
  CACCTTCCCAAGCTGGCTGGGGAAGCACTCGTCTACAGGCAAACATGATCGTATTGTTCAGGACCTGGCC
  TTGCATATGAGTCTCAGAACTTACTCCAGCAAAAGGACTGTAAACATGGATTATCTGTCGCTTCTAAGGG
  ATGCACTTGTACAGCCCTTGACCTCACAAGGAGTAGACGGAGTACAGGATGTTGTTGCACTTATGGACAC
  ATATTATTTGATGAAAGAAGACTTTGAGAATATCATGGAAATCAGCAGCTGGGGTGGCAAACCTAGTCCC
  TTTTCAAAGCTGGATCCCAAGGTGAAAGCAGCCTTCACAAGAGCTTACAATAAGGAAGCCCACCTTACTC
  CATACTCACTTCAAGCTATAAAGGCATCTAGACACAGCACAAGCCCATCCCTGGATTCGGAATACAATGA
  AGAATTAAATGAAGATGACTCTCAATCTGATGAGAAAGACCAAGATGCTATAGAAACTGATGCCATGATC
  AAGAAAAAGACAAAATCTTCAAAGCCTTCAAAACCAGAAAAAGATAAGGAGCCCAGAAAAGGAAAAGGAA
  AAAGTTCGAAGAAATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204747
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204747.1, NP_001191676.1
• RefSeq Size: 4899 bp
• RefSeq ORF: 3447 bp
• Locus ID: 5981
• Cytogenetics: 4p14
• Protein Families: Transcription Factors
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Gene Summary: 'This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]'
  Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1, resulting in an isoform (2) that is 1 aa longer than isoform 1.