UTY (NM_001258255) Human Tagged ORF Clone

CAT#: RG235250

UTY (GFP-tagged) - Homo sapiens ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (UTY), transcript variant 10

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UTY
• Synonyms: KDM6AL; KDM6C; UTY1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235250 representing NM_001258255
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAGAAAATGGCGG
  AAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCGAGGAAAGGGAGGCGCTTGG
  TGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTA
  GGCAAGGCTGTTCGCTGCTACGAATCTTTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGACTTCTTTT
  GCCAATTAGGTCACTTCAACCTCTTGTTGGAAGATTATTCAAAAGCATTATCTGCATATCAGAGATATTA
  CAGTTTACAGGCTGACTACTGGAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTAC
  AATGCATTTCATTGGGCAATTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTTTGTCGAGCCA
  AGGAAATTCATTTACGACTTGGGCTCATGTTCAAAGTGAACACAGACTACAAGTCTAGTTTAAAGCATTT
  TCAGTTAGCCTTGATTGACTGTAATCCATGTACTTTGTCCAATGCTGAAATTCAATTTCATATTGCCCAT
  TTGTATGAAACCCAGAGGAAGTATCATTCTGCAAAGGAGGCATATGAACAACTTTTGCAGACAGAAAACC
  TTCCTGCACAAGTAAAAGCAACTGTATTGCAACAGTTAGGTTGGATGCATCATAATATGGATCTAGTAGG
  AGACAAAGCCACAAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCTTTGGAGGCAGATCCTAATTCT
  GGCCAATCGTGGTATTTTCTTGGAAGGTGTTATTCAAGTATTGGGAAAGTTCAGGATGCCTTTATATCTT
  ACAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGGTGTGTTGTATCAGCA
  GCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTGTGCTGTACAATTGGACCATGGGCATGCCGCA
  GCCTGGATGGACCTAGGTACTCTCTATGAATCCTGCAATCAACCTCAAGATGCCATTAAATGCTACCTAA
  ATGCAGCTAGAAGCAAACGTTGTAGTAATACCTCTACGCTTGCTGCAAGAATTAAATTTCTACAGGCTCA
  GTTGTGTAACCTTCCACAAAGTAGTCTACAGAATAAAACTAAATTACTTCCTAGTATTGAGGAGGCATGG
  AGCCTACCAATCCCCGCAGAGCTTACCTCCAGGCAGGGTGCCATGAACACAGCACAGCAGAATGGTTCTG
  ATAACTGGAATGGTGGCCAGAGTCTTTCACATCATCCAGTACAGCAAGTTTATTCGTTGTGTTTGACACC
  ACAGAAATTACAGCACTTGGAACAACTGCGAGCAAATAGAGATAATTTAAATCCAGCACAGAAGCATCAG
  CTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGCAGCAAATGAGACACAAAGAAGTTGCTCAGGGGCTTC
  ATAAAAGTCAGAGTTCATGTTTGTCAGGACCTAATGAAGAACAACCTCTGTTTTCCACTGGGTCAGCCCA
  GTATCACCAGGCAACTAGCACTGGTATTAAGAAGGCGAATGAACATCTCACTCTGCCTAGTAATTCAGTA
  CCACAGGGGGATGCTGACAGTCACCTCTCCTGTCATACTGCTACCTCAGGTGGACAACAAGGCATTATGT
  TTACCAAAGAGAGCAAGCCTTCAAAAAATAGATCCTTGGTGCCTGAAACAAGCAGGCATACTGGAGACAC
  ATCTAATGGCTGTGCTGATGTCAAGGGACTTTCTAATCATGTTCATCAGTTGATAGCAGATGCTGTTTCC
  AGTCCTAACCATGGAGATTCACCAAATTTATTAATTGCAGACAATCCTCAGCTCTCTGCTTTGTTGATTG
  GAAAAGCCAATGGCAATGTGGGTACTGGAACCTGTGACAAAGTGAATAATATTCACCCAGCTGTTCATAC
  AAAGACTGATCATTCTGTTGCCTCTTCACCCTCTTCAGCCATTTCCACAGCAACACCTTCTCCTAAATCC
  ACTGAGCAGAGAAGCATAAACAGTGTTACCAGCCTTAACAGTCCTCACAGTGGATTACACACAGTCAATG
  GAGAGGGGCTGGGGAAGTCACAGAGCTCTACAAAAGTAGACCTGCCTTTAGCTAGCCACAGATCTACTTC
  TCAGATCTTACCATCAATGTCAGTGTCTATATGCCCCAGTTCAACAGAAGTTCTGAAAGCATGCAGGAAT
  CCAGGTAAAAATGGCTTGTCTAATAGCTGCATTTTGTTAGATAAATGTCCACCTCCAAGACCACCAACTT
  CACCATACCCACCCTTGCCAAAGGACAAGTTGAATCCACCCACACCTAGTATTTACTTGGAAAATAAACG
  TGATGCTTTCTTTCCTCCATTACATCAATTTTGTACAAATCCAAAAAACCCTGTTACAGTAATACGTGGC
  CTTGCTGGAGCTCTTAAATTAGATCTTGGACTTTTCTCTACCAAAACTTTGGTAGAAGCTAACAATGAAC
  ATATGGTAGAAGTGAGGACACAGTTGCTGCAACCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAA
  AATCTGGCGTTGTGAAAGCAATAGATCTCATACTACAATTGCCAAATACGCACAATACCAGGCTTCCTCC
  TTCCAGGAATCATTGAGAGAAGAAAATGAGAAAAGAACACAACACAAAGATCATTCAGATAACGAATCCA
  CATCTTCAGAGAATTCTGGAAGGAGAAGGAAAGGACCTTTTAAAACCATAAAATTTGGGACCAACATTGA
  CCTCTCTGATAACAAAAAGTGGAAGTTGCAGTTACATGAACTGACTAAACTTCCTGCTTTTGCGCGTGTG
  GTGTCAGCAGGAAATCTTCTAACCCATGTTGGGCATACCATTCTGGGCATGAATACAGTACAACTGTATA
  TGAAAGTTCCAGGGAGTCGGACACCAGGTCACCAAGAAAATAACAACTTCTGCTCTGTTAACATAAATAT
  TGGTCCAGGAGATTGTGAATGGTTTGTTGTACCTGAAGATTATTGGGGTGTTCTGAATGACTTCTGTGAA
  AAAAATAATTTGAATTTTTTAATGAGTTCTTGGTGGCCCAACCTTGAAGATCTTTATGAAGCAAATGTCC
  CTGTGTATAGATTTATTCAGCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTGCATTGGGTTCA
  AGCTGTTGGCTGGTGCAATAACATTGCCTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCA
  GTGGAACGGTATGAATGGAACAAATTGAAAAGTGTGAAGTCACCAGTACCCATGGTGCATCTTTCCTGGA
  ATATGGCACGAAATATCAAAGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTTTGAAAAT
  TCTGAAGCAATATCAGACATTGAGAGAAGCTCTTGTTGCAGCAGGAAAAGAGGTTATATGGCATGGGCGG
  ACAAATGATGAACCAGCTCATTACTGTAGCATTTGTGAGGTGGAGGTTTTTAATCTGCTTTTTGTCACTA
  ATGAAAGCAATACTCAAAAAACCTACATAGTACATTGCCATGATTGTGCACGAAAAACAAGCAAAAGTTT
  GGAAAATTTTGTGGTGCTCGAACAGTACAAAATGGAGGACCTAATCCAAGTTTATGATCAATTTACACTA
  GCTCTTTCATTATCATCCTCATCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235250 representing NM_001258255
  Red=Cloning site Green=Tags(s)
  
  MKSCAVSLTTAAVAFGDEAKKMAEGKASRESEEESVSLTVEEREALGGMDSRLFGFVRLHEDGARTKTLL
  GKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYSKALSAYQRYYSLQADYWKNAAFLYGLGLVYFYY
  NAFHWAIKAFQDVLYVDPSFCRAKEIHLRLGLMFKVNTDYKSSLKHFQLALIDCNPCTLSNAEIQFHIAH
  LYETQRKYHSAKEAYEQLLQTENLPAQVKATVLQQLGWMHHNMDLVGDKATKESYAIQYLQKSLEADPNS
  GQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAA
  AWMDLGTLYESCNQPQDAIKCYLNAARSKRCSNTSTLAARIKFLQAQLCNLPQSSLQNKTKLLPSIEEAW
  SLPIPAELTSRQGAMNTAQQNGSDNWNGGQSLSHHPVQQVYSLCLTPQKLQHLEQLRANRDNLNPAQKHQ
  LEQLESQFVLMQQMRHKEVAQGLHKSQSSCLSGPNEEQPLFSTGSAQYHQATSTGIKKANEHLTLPSNSV
  PQGDADSHLSCHTATSGGQQGIMFTKESKPSKNRSLVPETSRHTGDTSNGCADVKGLSNHVHQLIADAVS
  SPNHGDSPNLLIADNPQLSALLIGKANGNVGTGTCDKVNNIHPAVHTKTDHSVASSPSSAISTATPSPKS
  TEQRSINSVTSLNSPHSGLHTVNGEGLGKSQSSTKVDLPLASHRSTSQILPSMSVSICPSSTEVLKACRN
  PGKNGLSNSCILLDKCPPPRPPTSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPKNPVTVIRG
  LAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWRCESNRSHTTIAKYAQYQASS
  FQESLREENEKRTQHKDHSDNESTSSENSGRRRKGPFKTIKFGTNIDLSDNKKWKLQLHELTKLPAFARV
  VSAGNLLTHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEDYWGVLNDFCE
  KNNLNFLMSSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGPLTACQYKLA
  VERYEWNKLKSVKSPVPMVHLSWNMARNIKVSDPKLFEMIKYCLLKILKQYQTLREALVAAGKEVIWHGR
  TNDEPAHYCSICEVEVFNLLFVTNESNTQKTYIVHCHDCARKTSKSLENFVVLEQYKMEDLIQVYDQFTL
  ALSLSSSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258255
• ORF Size: 3804 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258255.1, NP_001245184.1
• RefSeq Size: 6289 bp
• RefSeq ORF: 3807 bp
• Locus ID: 7404
• Cytogenetics: Yq11.221
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. The encoded protein is also a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. A large number of alternatively spliced transcripts have been observed for this gene, but the full length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2012]'