JLP (SPAG9) (NM_001130527) Human Tagged ORF Clone

CAT#: RG235262

SPAG9 (GFP-tagged) - Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SPAG9
• Synonyms: CT89; HLC-6; HLC4; HLC6; JIP-4; JIP4; JLP; PHET; PIG6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235262 representing NM_001130527
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGGACGGTGTGGTGTATCAGGAGGAGCCCGGCGGCTCCGGGGCCGTGATGTCGGAGCGGG
  TGTCCGGCCTGGCCGGCTCCATCTACCGCGAGTTCGAGCGGCTTATCGGGCGCTATGACGAGGAGGTGGT
  CAAAGAGCTGATGCCGCTGGTGGTGGCTGTGCTGGAGAACCTGGACTCGGTGTTCGCGCAGGACCAGGAG
  CACCAGGTGGAGCTGGAGCTGCTGCGGGACGACAACGAGCAGCTCATCACCCAGTACGAGCGGGAGAAGG
  CGCTGCGCAAGCACGCTGAGGAGAAATTCATTGAATTTGAAGACTCTCAAGAACAGGAAAAAAAGGACTT
  ACAGACCCGAGTGGAATCTTTAGAATCTCAAACAAGACAACTTGAGCTGAAAGCGAAAAACTATGCTGAC
  CAGATTAGCAGACTTGAAGAAAGAGAAGCAGAACTGAAGAAGGAATATAATGCATTACATCAAAGACACA
  CTGAGATGATCCATAATTATATGGAACATTTAGAAAGAACAAAACTTCATCAGCTCTCAGGGAGTGATCA
  ACTAGAATCCACAGCTCATAGTAGAATTAGAAAAGAACGCCCTATATCATTAGGAATTTTCCCATTACCT
  GCTGGAGATGGATTGCTTACACCTGATGCTCAGAAAGGAGGAGAGACCCCTGGATCTGAGCAATGGAAAT
  TTCAGGAATTAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAGCTTTCTGATGTTAGCCAAGGCGG
  ATCTAAAGCTACCACTCCAGCATCAACAGCTAATTCAGATGTGGCAACAATTCCTACTGATACTCCCTTA
  AAGGAAGAAAACGAAGGATTTGTGAAGGTTACAGATGCGCCAAATAAATCAGAGATAAGCAAACACATTG
  AAGTACAGGTAGCCCAGGAAACTAGAAATGTATCTACTGGCTCTGCTGAAAATGAAGAAAAGTCAGAAGT
  TCAAGCAATCATCGAATCTACTCCTGAGCTGGATATGGACAAAGATCTCAGTGGATATAAAGGTTCAAGC
  ACTCCCACCAAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAACTGTCTT
  CAGCTGGCTCAGGCCTAATAGGAGATGTGGATGAAGGAGCAGATTTACTAGGAATGGGTCGGGAAGTTGA
  GAATCTTATATTAGAAAATACACAACTGTTGGAAACCAAAAATGCTTTGAACATAGTGAAGAATGATTTG
  ATAGCAAAAGTGGATGAACTGACCTGTGAGAAAGATGTGCTGCAAGGGGAATTGGAGGCTGTGAAGCAAG
  CCAAACTGAAACTAGAGGAAAAGAACAGAGAATTGGAGGAAGAGCTTAGGAAAGCTCGGGCAGAAGCTGA
  AGATGCAAGGCAAAAAGCAAAAGATGACGATGATAGTGATATTCCCACAGCCCAGAGGAAACGGTTTACT
  AGAGTAGAAATGGCCCGTGTTCTCATGGAGCGAAACCAGTATAAAGAGAGATTGATGGAGCTTCAGGAAG
  CTGTTCGATGGACAGAGATGATTCGGGCATCACGAGAAAATCCAGCCATGCAGGAAAAAAAAAGGTCAAG
  CATTTGGCAGTTTGTGCCAACTCGTTTCAGCCGACTTTTCAGCTCCTCAAGTAACACGACTAAGAAGCCT
  GAACCACCTGTTAATCTGAAGTACAATGCACCCACGTCTCATGTTACTCCGTCCGTCAAGAAAAGAAGCA
  GCACCTTATCTCAGCTCCCTGGGGATAAGTCCAAAGCCTTTGATTTCCTTAGTGAAGAAACTGAAGCTAG
  TTTAGCCTCACGCAGAGAACAAAAGAGAGAGCAGTATCGTCAGGTAAAAGCACATGTTCAGAAGGAAGAC
  GGTAGAGTGCAGGCTTTTGGCTGGAGTCTGCCTCAGAAGTACAAACAGGTAACCAATGGTCAAGGTGAAA
  ATAAGATGAAAAATTTACCTGTGCCTGTCTATCTCAGACCTCTGGATGAAAAAGATACATCAATGAAGCT
  GTGGTGTGCTGTTGGAGTCAATTTATCTGGTGGGAAGACCAGAGATGGTGGTTCTGTTGTTGGAGCAAGT
  GTATTTTACAAGGATGTTGCTGGTTTGGATACAGAAGGCAGTAAACAGCGAAGTGCCTCTCAGAGTAGTT
  TAGATAAGTTAGATCAGGAACTTAAGGAACAGCAGAAGGAGTTAAAAAATCAAGAAGAATTATCCAGTCT
  AGTTTGGATCTGTACCAGCACTCATTCGGCTACAAAAGTTCTTATTATTGATGCTGTTCAACCTGGCAAC
  ATCCTAGACAGTTTCACTGTTTGCAACTCTCATGTTCTGTGCATTGCAAGTGTGCCAGGTGCACGAGAAA
  CAGACTACCCTGCAGGAGAAGATCTTTCAGAATCTGGTCAGGTAGACAAAGCATCTTTATGTGGAAGTAT
  GACAAGCAACAGCTCAGCAGAGACAGACAGCCTGTTAGGAGGCATCACAGTGGTTGGTTGTTCTGCAGAA
  GGTGTGACGGGAGCTGCCACTTCCCCTAGTACAAATGGTGCTTCTCCAGTGATGGATAAACCACCAGAAA
  TGGAAGCAGAAAATAGTGAGGTTGATGAAAATGTTCCAACAGCAGAAGAAGCAACTGAAGCTACAGAAGG
  GAATGCGGGGTCAGCTGAAGACACAGTGGACATCTCCCAAACTGGCGTCTACACAGAGCATGTCTTTACA
  GATCCTTTGGGAGTTCAGATCCCAGAAGACCTCTCCCCAGTGTATCAGTCGAGCAATGACTCAGATGCAT
  ATAAAGATCAAATATCAGTACTGCCAAATGAACAAGACTTGGTGAGAGAAGAAGCCCAGAAAATGAGTAG
  TCTTTTACCAACTATGTGGCTTGGAGCTCAAAATGGCTGTTTGTATGTCCATTCATCTGTAGCCCAGTGG
  AGGAAATGTCTCCATTCCATTAAACTTAAAGATTCGATTCTCAGTATTGTACACGTGAAGGGAATCGTGT
  TAGTAGCCCTGGCTGACGGCACCCTTGCAATCTTTCACAGAGGAGTGGATGGGCAGTGGGATTTGTCAAA
  CTATCACCTCTTAGACCTTGGACGGCCTCATCATTCCATCCGTTGCATGACTGTGGTACATGACAAAGTC
  TGGTGTGGCTATAGGAACAAAATCTATGTGGTGCAGCCAAAGGCCATGAAAATAGAGAAATCTTTTGATG
  CACATCCCAGGAAGGAGAGCCAAGTGCGACAGCTTGCGTGGGTGGGGGATGGCGTGTGGGTCTCCATTCG
  CTTGGATTCTACGCTCCGTCTCTATCATGCACACACTTATCAACATCTACAGGATGTGGACATTGAGCCT
  TATGTAAGCAAAATGTTAGGTACTGGAAAACTGGGCTTCTCTTTTGTGAGAATTACAGCTCTTATGGTGT
  CTTGTAATCGTTTGTGGGTGGGGACAGGAAATGGTGTCATTATCTCCATCCCATTGACAGAAACAAATAA
  AACCTCAGGTGTACCAGGAAATCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAAAACAGTGATAAA
  GTGACTCCAGGGACATTTATACCCTATTGTTCAATGGCACATGCACAGCTTTGCTTCCATGGGCACCGGG
  ATGCTGTGAAATTCTTTGTGGCAGTCCCAGGTCAAGTCATCAGCCCACAAAGTAGCAGTAGTGGCACGGA
  TCTGACGGGTGACAAAGCAGGGCCATCTGCACAGGAGCCTGGTAGTCAGACGCCCTTGAAGTCTATGCTT
  GTCATCAGTGGAGGAGAGGGCTACATCGACTTCCGAATGGGTGATGAAGGTGGAGAATCAGAACTTCTTG
  GAGAGGATCTTCCACTTGAACCTTCTGTCACCAAAGCAGAAAGGAGTCACTTGATAGTGTGGCAAGTGAT
  GTATGGCAATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235262 representing NM_001130527
  Red=Cloning site Green=Tags(s)
  
  MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQE
  HQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYAD
  QISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLP
  AGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPL
  KEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSS
  TPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDL
  IAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFT
  RVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFVPTRFSRLFSSSSNTTKKP
  EPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKED
  GRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGAS
  VFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGN
  ILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAE
  GVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFT
  DPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQW
  RKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKV
  WCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEP
  YVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDK
  VTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSML
  VISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130527
• ORF Size: 3933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130527.2, NP_001123999.1
• RefSeq Size: 8243
• RefSeq ORF: 3936
• Locus ID: 9043
• Gene Summary: This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]