l Afadin (AFDN) (NM_001207008) Human Tagged ORF Clone

CAT#: RG235333

MLLT4 (GFP-tagged) - Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 (MLLT4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AFDN
• Synonyms: AF6; l-afadin; MLL-AF6; MLLT4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235333 representing NM_001207008
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGGGCGGCCGTGACGAGGAGCGGCGGAAGCTGGCCGACATCATCCACCACTGGAACGCCAACC
  GGCTGGACCTGTTCGAGATCAGCCAGCCGACCGAGGATTTGGAGTTCCATGGAGTGATGAGATTTTATTT
  TCAAGATAAAGCTGCTGGAAACTTTGCAACAAAATGTATTCGGGTCTCTAGTACTGCCACCACTCAAGAT
  GTAATCGAAACGCTCGCGGAGAAATTTCGACCTGATATGCGAATGCTGTCCTCTCCCAAGTATTCACTCT
  ATGAAGTGCATGTCAGCGGAGAAAGAAGATTGGATATAGATGAGAAACCTCTAGTTGTACAACTGAATTG
  GAACAAAGATGATCGGGAAGGCAGATTTGTTCTTAAGAATGAGAATGACGCCATTCCTCCTAAGGCTCAA
  AGTAATGGACCTGAAAAGCAGGAAAAAGAAGGGGTTATCCAGAACTTCAAGAGAACTCTCTCAAAGAAAG
  AAAAGAAGGAAAAAAAGAAGAGAGAAAAAGAGGCATTGCGACAGGCATCTGATAAAGATGATAGACCTTT
  CCAAGGGGAGGATGTTGAAAATTCTCGACTGGCTGCTGAGGTTTACAAAGACATGCCGGAAACCAGCTTT
  ACTCGAACCATTTCTAATCCTGAGGTGGTTATGAAACGACGGAGGCAGCAAAAATTGGAAAAGAGAATGC
  AGGAATTTCGGAGCTCAGATGGGCGGCCTGATTCAGGTGGAACATTGAGAATTTATGCAGATAGTTTAAA
  ACCAAATATTCCCTACAAGACAATCCTGCTGTCTACTACAGATCCTGCAGACTTTGCTGTGGCTGAAGCT
  TTAGAGAAGTATGGTCTGGAAAAAGAAAACCCTAAGGATTACTGCATCGCCCGGGTTATGCTTCCTCCTG
  GAGCCCAGCATTCTGATGAAAAGGGTGCTAAAGAAATTATTCTTGATGATGATGAGTGTCCTTTACAAAT
  CTTCAGGGAATGGCCAAGTGACAAAGGGATTTTAGTCTTTCAGTTGAAGAGGAGGCCACCAGACCACATC
  CCAAAGAAAACCAAGAAACACTTGGAAGGCAAGACACCCAAGGGAAAGGAGAGAGCTGACGGGTCTGGCT
  ATGGCTCCACCCTTCCTCCGGAGAAGCTGCCCTATTTAGTAGAGTTAAGCCCAGATGGTTCTGACTCTAG
  AGATAAGCCAAAGCTTTACCGCCTTCAGTTAAGTGTTACTGAAGTTGGGACAGAAAAGTTGGATGACAAC
  TCTATCCAGTTGTTTGGCCCAGGAATTCAGCCCCATCACTGTGACCTTACCAACATGGATGGAGTGGTCA
  CTGTGACGCCCAGAAGTATGGACGCAGAAACCTACGTGGAAGGCCAGCGCATCTCAGAAACCACCATGCT
  GCAGAGTGGCATGAAAGTGCAGTTTGGGGCGTCCCATGTATTTAAGTTTGTGGACCCCAGTCAGGATCAT
  GCTCTTGCAAAAAGATCTGTGGATGGAGGCCTGATGGTTAAGGGCCCAAGACATAAACCTGGAATTGTTC
  AGGAGACAACTTTTGATTTGGGAGGAGATATTCATAGTGGGACAGCATTACCGACAAGCAAGAGCACCAC
  TAGGCTGGACAGCGACAGAGTGTCGTCTGCCTCTAGCACAGCCGAGCGGGGAATGGTGAAGCCGATGATC
  AGAGTAGAACAGCAGCCAGATTATCGCAGGCAAGAAAGCAGAACACAGGATGCTTCTGGGCCTGAGCTGA
  TACTACCTGCAAGCATTGAATTCAGGGAAAGTTCTGAAGATTCATTTTTGTCTGCCATTATAAATTATAC
  TAATAGCTCTACAGTCCACTTTAAGTTGTCCCCTACATATGTATTATATATGGCATGCCGGTATGTATTG
  TCCAACCAGTACAGACCTGACATCAGCCCTACAGAGCGCACACATAAAGTCATTGCAGTCGTCAACAAGA
  TGGTGAGCATGATGGAGGGTGTCATCCAGAAACAGAAGAATATTGCAGGGGCACTTGCCTTCTGGATGGC
  AAATGCATCTGAACTTCTCAACTTCATTAAGCAAGACCGAGACCTTAGTCGGATCACACTGGATGCTCAA
  GATGTTTTAGCACATTTGGTTCAAATGGCATTTAAATACTTGGTTCACTGTCTTCAATCAGAACTTAATA
  ATTACATGCCAGCCTTTCTAGATGACCCTGAAGAGAACAGTCTGCAACGACCAAAAATAGATGATGTGCT
  GCACACGCTCACAGGAGCCATGTCCTTGCTACGACGCTGCAGAGTCAATGCCGCCCTGACCATCCAGCTC
  TTCTCTCAGCTCTTCCACTTCATCAATATGTGGCTGTTCAATAGATTGGTGACCGACCCAGATTCGGGGC
  TGTGCTCCCATTACTGGGGTGCGATTATCCGTCAGCAGTTGGGCCATATTGAAGCCTGGGCTGAGAAGCA
  GGGGCTGGAACTGGCTGCGGACTGTCATCTGAGCAGGATCGTGCAGGCAACGACTTTGCTTACCATGGAT
  AAGTATGCACCTGATGACATTCCAAATATAAACAGCACCTGCTTTAAGTTAAATTCATTACAACTTCAAG
  CCTTATTACAGAACTATCACTGTGCACCTGATGAGCCTTTTATCCCAACGGATCTTATAGAAAATGTAGT
  GACTGTGGCTGAAAACACTGCCGATGAGCTGGCCCGCAGTGATGGAAGGGAAGTGCAGTTGGAGGAGGAT
  CCTGATCTGCAGCTGCCGTTTCTTTTGCCAGAAGATGGTTATTCTTGTGATGTTGTCAGAAACATTCCAA
  ATGGTTTACAAGAATTTTTAGACCCTCTGTGCCAGAGAGGATTTTGCAGGTTAATTCCTCACACACGTTC
  ACCAGGTACTTGGACAATATATTTTGAAGGTGCAGATTATGAAAGTCACCTTCTGCGTGAGAACACAGAG
  CTGGCTCAGCCTCTGAGGAAAGAACCTGAAATAATCACTGTGACCCTAAAAAAGCAGAATGGAATGGGCC
  TTAGCATTGTTGCAGCAAAGGGTGCTGGTCAAGATAAACTAGGAATCTATGTGAAGTCGGTTGTGAAAGG
  AGGTGCTGCAGATGATGGACGTCTAGCTGCAGGTGATCAGCTCCTCAGTGTGGATGGACGAAGTCTGGTT
  GGACTCTCTCAGGAAAGGGCGGCAGAACTCATGACAAGAACAAGCTCTGTGGTGACACTGGAAGTAGCAA
  AGCAGGGTGCCATCTACCACGGTCTGGCCACCCTTCTCAATCAGCCATCCCCCATGATGCAGAGAATTTC
  AGATCGTCGTGGCTCAGGTAAACCCCGACCAAAGAGTGAAGGCTTTGAGCTCTATAATAATTCAACTCAA
  AATGGGTCTCCTGAGAGTCCTCAGCTGCCTTGGGCAGAATATAGTGAACCAAAGAAATTGCCTGGTGATG
  ACAGACTGATGAAAAATAGAGCTGATCACCGTTCCAGCCCCAACGTAGCAAATCAGCCTCCTAGTCCTGG
  AGGGAAAAGTGCATATGCCTCTGGAACAACAGCGAAGATAACATCTGTCTCTACTGGAAACCTCTGCACT
  GAGGAGCAGACGCCTCCGCCTAGACCTGAAGCCTACCCCATCCCCACTCAGACGTACACCAGAGAGTATT
  TTACCTTCCCAGCTTCCAAATCCCAGGATCGGATGGCTCCTCCTCAGAACCAGTGGCCAAATTATGAGGA
  AAAGCCACATATGCACACAGATAGTAATCATTCCAGTATTGCAATTCAGCGTGTTACACGTTCCCAAGAA
  GAACTTCGAGAAGATAAAGCTTACCAACTTGAGCGGCATCGAATAGAGGCAGCTATGGACCGAAAGTCTG
  ATAGTGATATGTGGATAAATCAGAGCTCCTCACTGGACTCCAGTACCTCTAGCCAGGAGCATCTGAACCA
  TTCCTCTAAGTCGGTCACCCCTGCTTCCACACTGACCAAAAGTGGCCCTGGCCGTTGGAAAACACCAGCA
  GCCATACCGGCCACCCCTGTGGCCGTCTCCCAGCCAATCCGAACAGACCTGCCTCCGCCACCCCCGCCAC
  CTCCAGTCCACTATGCCGGTGATTTCGATGGAATGTCCATGGATTTGCCTCTCCCACCACCCCCTTCCGC
  CAACCAGATAGGGCTGCCGTCTGCGCAGGTGGCTGCTGCTGAACGGAGAAAGAGAGAAGAACATCAGCGT
  TGGTATGAGAAGGAGAAGGCCCGCCTGGAGGAGGAGCGGGAGAGGAAGCGGAGAGAGCAGGAGAGGAAGT
  TGGGCCAGATGCGCACTCAGTCCTTAAACCCTGCTCCGTTTTCTCCCCTGACTGCACAGCAGATGAAGCC
  CGAAAAGCCTTCCACACTCCAGCGGCCACAGGAAACAGTCATTCGGGAGCTGCAGCCTCAGCAGCAGCCC
  CGCACGATCGAGCGCAGAGACTTGCAGTACATTACAGTCAGCAAAGAGGAGCTTTCCTCGGGGGACAGTC
  TGTCCCCCGACCCGTGGAAGCGGGACGCCAAGGAGAAGCTGGAGAAGCAGCAGCAGATGCACATCGTGGA
  CATGCTGAGCAAGGAGATCCAGGAGCTCCAGAGCAAACCGGACCGCAGCGCCGAGGAGAGCGACCGGCTG
  CGCAAGCTCATGCTGGAGTGGCAGTTCCAGAAGAGACTCCAGGAGTCGAAGCAGAAGGACGAAGATGACG
  AGGAGGAGGAGGACGATGATGTGGACACCATGCTGATCATGCAGCGCCTGGAGGCTGAACGAAGAGCGAG
  GTTGCAGGACGAGGAGCGGAGGCGGCAGCAGCAGTTAGAAGAGATGCGCAAGCGGGAAGCGGAAGACCGA
  GCGAGGCAAGAGGAAGAGCGCCGGCGGCAGGAGGAGGAGCGAACAAAACGAGACGCTGAAGAAAAGAGGC
  GACAGGAAGAAGGGTATTACAGCCGCCTGGAAGCCGAGAGGCGCAGACAGCACGACGAGGCGGCGCGCAG
  GTTGCTGGAGCCCGAGGCGCCCGGACCAAACTCTTACCCAGGATCTACTGGAGCAGCTGTTGGAGCCCAT
  GACGCCTGTCGGGATGCAAAAGAGAAGCGCTCTAAAAGCCAAGATGCAGATTCACCTGGAAGTTCTGGGG
  CCCCTGAAAACTTGACATTCAAGGAACGCCAGCGTCTTTTTTCACAAGGTCAAGATGTATCCAATAAAGT
  GAAAGCTTCTCGTAAATTAACAGAACTGGAGAATGAACTGAACACAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235333 representing NM_001207008
  Red=Cloning site Green=Tags(s)
  
  MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQD
  VIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQ
  SNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSF
  TRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEA
  LEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHI
  PKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDN
  SIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDH
  ALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMI
  RVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVL
  SNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQ
  DVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQL
  FSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMD
  KYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEED
  PDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTE
  LAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADDGRLAAGDQLLSVDGRSLV
  GLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQ
  NGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCT
  EEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQE
  ELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPA
  AIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQR
  WYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQP
  RTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRL
  RKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDR
  ARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAH
  DACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001207008
• ORF Size: 5229 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001207008.1, NP_001193937.1
• RefSeq Size: 7459 bp
• RefSeq ORF: 5232 bp
• Locus ID: 4301
• Cytogenetics: 6q27
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Leukocyte transendothelial migration, Tight junction
• Gene Summary: 'This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011]'