CREM (NM_001267565) Human Tagged ORF Clone

CAT#: RG235903

CREM (GFP-tagged) - Human cAMP responsive element modulator (CREM), transcript variant 26

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CREM
• Synonyms: CREM-2; hCREM-2; ICER
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235903 representing NM_001267565.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGTGGCATCAGCATAATCTATGTTTCAGGCGTCCTATAGAAGAGGATTATTCTTCAGGGGATGTG
  GAAGAAAAGGCACAGTCTCGCTCTGTCATCCAGGCTGGAGTGCGGTGGCATGATCTCAGCTCACTGCAA
  CCTCGAACTCCTGGGTTCAAGAAATTCTCATGCCTCAGCCTCATGAGTAGCTGGGATTACAGGAAAATA
  CTGAATGAACTGTCCTCTGATGTGCCTGGTGTTCCCAAGATTGAAGAAGAGAGATCAGAGGAAGAAGGA
  ACACCACCTAGTATTGCTACCATGGCAGTACCAACTAGCATATATCAGACTAGCACGGGGCAATACAGT
  ATGTATGCTGCAATTCGATATGATACAGTGCTAGCTTTAAGTCTTCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG235903
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MWWHQHNLCFRRPIEEDYSSGDVEEKAQSRSVIQAGVRWHDLSSLQPRTPGFKKFSCLSLMSSWDYRKI
  LNELSSDVPGVPKIEEERSEEEGTPPSIATMAVPTSIYQTSTGQYSMYAAIRYDTVLALSLL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267565
• ORF Size: 393 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001267565.1, NP_001254494.1
• RefSeq Size: 1118 bp
• RefSeq ORF: 396 bp
• Locus ID: 1390
• Cytogenetics: 10p11.21
• Protein Families: Druggable Genome, Transcription Factors
• MW: 15.4 kDa
• Gene Summary: 'This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008]'