NCE2 (UBE2F) (NM_001278308) Human Tagged ORF Clone

CAT#: RG236197

UBE2F (GFP-tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: UBE2F
• Synonyms: NCE2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236197 representing NM_001278308.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGTCTCAAAGGGTCCCGGACGGCAGCCACAGCG
  TCCGACTCGACTCGGAGGGTTTCTGTGAGAGACAAATTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCT
  AATTTACCTTGTACATGTAAAGTGCATTTTCCTGATCCAAACAAGCTTCATTGTTTTCAGCTAACAGTA
  ACCCCAGATGAGGGTTACTACCAGGGTGGAAAATTTCAGTTTGAAACTGAAGTTCCCGATGCGTACAAC
  ATGGTGCCTCCCAAAGTGAAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAATA
  TGTCTGAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACAAGAACATTAAAGGAT
  GTCGTTTGGGGATTAAACTCTTTGTTTACTGAGGACTTCCGGAATAAAGTGGATGACTACATCAAACGT
  TATGCCAGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236197
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTV
  TPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKD
  VVWGLNSLFTEDFRNKVDDYIKRYAR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278308
• ORF Size: 492 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278308.1, NP_001265237.1
• RefSeq Size: 2143 bp
• RefSeq ORF: 495 bp
• Locus ID: 140739
• Cytogenetics: 2q37.3
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 19.1 kDa