FTSJ1 (NM_001282157) Human Tagged ORF Clone

CAT#: RG236456

FTSJ1 (GFP-tagged) - Human FtsJ RNA methyltransferase homolog 1 (E. coli) (FTSJ1), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: FTSJ1
• Synonyms: CDLIV; JM23; MRX9; MRX44; SPB1; TRMT7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236456 representing NM_001282157.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTCTGAACATTGCTACACATGTCCTGAAGCCAGGGGGCTGCTTTGTGGCCAAGATATTCCGAGGC
  CGGGATGTGACGCTCCTCTACAGCCAGCTGCAGGTCTTCTTCTCCAGCGTGCTGTGTGCCAAGCCCAGG
  AGCAGCCGGAACTCTAGCATCGAGGCCTTCGCTGTCTGTCAGGGCTATGACCCTCCCGAGGGCTTCATC
  CCGGACCTGAGCAAACCCCTGCTGGACCATTCTTACGACCCAGATTTCAACCAGCTGGATGGTCCCACC
  CGCATCATTGTGCCTTTTGTGACCTGTGGGGACCTGAGCTCCTATGATTCGGACCGCAGTTACCCACTG
  GACCTAGAGGGCGGCTCAGAGTACAAGTACACTCCACCCACACAGCCCCCCATCTCGCCACCATACCAG
  GAGGCCTGCACGTTGAAGAGGAAGGGGCAGCTGGCCAAGGAGATCCGCCCCCAGGACTGCCCCATCAGC
  AGAGTGGACACGTTTCCCCAGCCCCTGGCCGCCCCTCAGTGCCACACCCTGCTGGCCCCTGAGATGGAA
  GACAATGAAATGAGTTGTTCACCT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236456
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MALNIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFI
  PDLSKPLLDHSYDPDFNQLDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQ
  EACTLKRKGQLAKEIRPQDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMSCSP
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282157
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282157.1, NP_001269086.1
• RefSeq Size: 1669 bp
• RefSeq ORF: 579 bp
• Locus ID: 24140
• Cytogenetics: Xp11.23
• MW: 21.7 kDa
• Gene Summary: This gene encodes a member of the methyltransferase superfamily. The encoded protein localizes to the nucleolus, binds to S-adenosylmethionine, and may be involved in the processing and modification of ribosomal RNA. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]