SSH2 (NM_001282130) Human Tagged ORF Clone

CAT#: RG236478

SSH2 (GFP-tagged) - Human slingshot protein phosphatase 2 (SSH2), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: SSH2
• Synonyms: SSH-2; SSH-2L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236478 representing NM_001282130.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTTTGGTCACGGTCCAGCGGTCACCTACCCCCAGCACCACCTCCAGCCCCTGCGCCTCGGAGGCA
  GACAGTGGGGAGGAAGAATGCCGGTCACAGCCCAGGAGCATCAGCGAGAGCTTTCTAACTGTCAAAGGT
  GCTGCCCTTTTTCTACCACGGGGAAATGGCTCATCCACACCAAGAATCAGCCACAGACGGAACAAGCAT
  GCAGGCGATCTCCAACAGCATCTCCAAGCAATGTTCATTTTACTCCGCCCAGAAGACAACATCAGGCTG
  GCTGTAAGACTGGAAAGTACTTACCAGAATCGAACACGCTATATGGTAGTGGTTTCAACTAATGGTAGA
  CAAGACACTGAAGAAAGCATCGTCCTAGGAATGGATTTCTCCTCTAATGACAGTAGCACTTGTACCATG
  GGCTTAGTTTTGCCTCTCTGGAGCGACACGCTAATTCATTTGGATGGTGATGGTGGGTTCAGTGTATCG
  ACGGATAACAGAGTTCACATATTCAAACCTGTATCTGTGCAGGCAATGTGGGTTGACAGGGATTCAAGG
  AACAAACACTGTGATGTACTATTGGTGGAAGAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236478
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MALVTVQRSPTPSTTSSPCASEADSGEEECRSQPRSISESFLTVKGAALFLPRGNGSSTPRISHRRNKH
  AGDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDSSTCTM
  GLVLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWVDRDSRNKHCDVLLVEE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282130
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282130.2
• RefSeq Size: 1024 bp
• RefSeq ORF: 588 bp
• Locus ID: 85464
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 22.1 kDa
• Gene Summary: This gene encodes a protein tyrosine phosphatase that plays a key role in the regulation of actin filaments. The encoded protein dephosphorylates and activates cofilin, which promotes actin filament depolymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]