ERCC8 (NM_001290285) Human Tagged ORF Clone

CAT#: RG236876

ERCC8 (GFP-tagged) - Human excision repair cross-complementation group 8 (ERCC8), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ERCC8
• Synonyms: CKN1; CSA; UVSS2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236876 representing NM_001290285.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGATACAAATACATTACAATTGGTACTAGAGGACCCAAAGTACAACTTTGTGACTTGAAGTCTGGA
  TCCTGTTCTCACATTCTACAGGGTCACAGACAAGAAATATTAGCAGTTTCCTGGTCTCCACGTTATGAC
  TATATCTTGGCAACAGCAAGTGCTGACAGTAGAGTAAAATTATGGGATGTGAGAAGAGCATCAGGATGT
  TTGATTACTCTTGATCAACATAATGGGAAAAAGTCACAAGCTGTTGAATCAGCAAACACTGCTCATAAT
  GGGAAAGTTAATGGCTTATGTTTTACAAGTGATGGACTTCACCTCCTCACTGTTGGTACAGATAATCGA
  ATGAGGCTCTGGAATAGTTCCAATGGAGAAAACACACTTGTGAACTATGGAAAAGTTTGTAATAACAGT
  AAAAAAGGATTGAAATTCACTGTCTCCTGTGGCTGCAGTTCAGAATTTGTTTTTGTACCATATGGTAGC
  ACCATTGCTGTTTATACAGTTTACTCAGGAGAACAGATAACTATGCTTAAGGGACATTATAAAACTGTT
  GACTGCTGTGTATTTCAGTCAAATTTCCAGGAACTTTATAGTGGTAGCAGAGACTGCAACATTCTGGCT
  TGGGTTCCATCCTTATATGAACCAGTTCCTGATGATGATGAGACTACAACAAAATCACAATTAAATCCG
  GCCTTTGAAGATGCCTGGAGCAGCAGTGATGAAGAAGGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236876
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGYKYITIGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRYDYILATASADSRVKLWDVRRASGC
  LITLDQHNGKKSQAVESANTAHNGKVNGLCFTSDGLHLLTVGTDNRMRLWNSSNGENTLVNYGKVCNNS
  KKGLKFTVSCGCSSEFVFVPYGSTIAVYTVYSGEQITMLKGHYKTVDCCVFQSNFQELYSGSRDCNILA
  WVPSLYEPVPDDDETTTKSQLNPAFEDAWSSSDEEG
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290285
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290285.1, NP_001277214.1
• RefSeq Size: 1962 bp
• RefSeq ORF: 732 bp
• Locus ID: 1161
• Cytogenetics: 5q12.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• MW: 27.3 kDa
• Gene Summary: 'This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]'