TGIF (TGIF1) (NM_001278686) Human Tagged ORF Clone

CAT#: RG236943

TGIF1 (GFP-tagged) - Human TGFB-induced factor homeobox 1 (TGIF1), transcript variant 11

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TGIF1
• Synonyms: HPE4; TGIF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236943 representing NM_001278686.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  RCATGGTTCTAGCGCAGAGCCGGGTGTCTGCCGGGGTGGGCTCCCCGCATTGTTCGGGCTCCGGCGGGG
  GCGGCTCTGATTCCTTTCCATGGCCCGCCTCCCACCCCGGGAATCCGCAGTGCTCCTTTTCCACGGCTT
  TTCTGGCGTCCCCCCGACTCTCCCGCGGCACTTTGGCCTACCTTCCCCCAGCGCCGTGGTCCTCCCTGG
  CGACCCCCTCTGCGCTCCTGGGGTCCTCCTGCGCCCCCCCTCCTCCACCGGCGCGCTGCCCACAGCCGC
  GTGCCCTCTCCCAGGAGCTGGGGACCAAGGCTGGGCCCCGCCGGCCGCATCGGTGGGAACTTCCGCGGT
  CCCCATCCCAGGGCGCACAGGGTCCAGCTCCTCGGCGCCGACTCCTGGAAACAATGAAAGGTATTGTTG
  CAGCATCTGGCAGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTTGGACCTTTCTTCATCCGCTG
  GCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCAAGGAGTCTGTGCAGATTCTTCGGGATTGGCTGT
  ATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGCGTTGCTGTCCCAGCAAACACACCTGT
  CTACGCTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGCAGGCTCCTCCCTGACATGCTGAGAAAGG
  ATGGCAAAGATCCAAATCAGTTCACAATTTCCCGCCGTGGGGCCAAGATTTCTGAAACGAGCTCTGTGG
  AGTCCGTGATGGGCATCAAAAACTTCATGCCAGCTCTAGAGGAGACCCCATTTCATTCCTGTACAGCTG
  GGCCAAACCCAACCCTAGGGAGGCCACTGTCTCCTAAGCCGTCATCCCCGGGATCAGTTTTGGCTCGTC
  CATCAGTGATCTGCCATACCACTGTGACTGCATTGAAAGATGTCCCTTTCTCTCTCTGCCAGTCGGTCG
  GTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAACTTCACAGACACCTCTCTCATGTACC
  CAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGTGGTCTTTTCAACACTCCTCCCCCTA
  CTCCACCGGACCTCAACCAGGACTTCAGTGGATTTCAGCTTCTAGTGGATGTTGCACTCAAACGGGCTG
  CAGAGATGGAGCTTCAGGCAAAACTTACAGCT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236943
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  XWF*RRAGCLPGWAPRIVRAPAGAALIPFHGPPPTPGIRSAPFPRLFWRPPDSPAALWPTFPQRRGPPWR
  PPLRSWGPPAPPLLHRRAAHSRVPSPRSWGPRLGPAGRIGGNFRGPHPRAHRVQLLGADSWKQ*KVLLQH
  LAVRLRMRTAWTFPWTFLHPLAQAREGEGATYPRSLCRFFGIGCMSTVTMPILQSKKKRCCPSKHTCLR
  YRSVTGSSTPAAGSSLTC*ERMAKIQISSQFPAVGPRFLKRALWSP*WASKTSCQL*RRPHFIPVQLGQTQP
  *GGHCLLSRHPRDQFWLVHQ*SAIPL*LH*KMSLSLSASRSVWDKTQIYSR*RPKTSQTPLSCTQRTLVNLDQV
  RIHRVVFSTLLPLLHRTSTRTSVDFSF*WMLHSNGLQRWSFRQNLQ
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278686
• ORF Size: 1211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278686.1, NP_001265615.1
• RefSeq Size: 1553 bp
• RefSeq ORF: 759 bp
• Locus ID: 7050
• Cytogenetics: 18p11.31
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• MW: 45.5 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2013]'