PRUNE (PRUNE1) (NM_001303243) Human Tagged ORF Clone

CAT#: RG236948

PRUNE (GFP-tagged) - Human prune exopolyphosphatase (PRUNE), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PRUNE1
• Synonyms: DRES-17; DRES17; H-PRUNE; HTCD37; NMIHBA; PRUNE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG236948 representing NM_001303243.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGATTGACCTCCATGCATTATACCAGGCTGGCCAACTCACCCTCATCCTTGTCGACCATCATATCT
  TATCCAAGACTGACCACTGAGCAGATGCTGAGAAAAGACCAGAAGACTATCTATAGACAAGGCGTCAAG
  GTGGCCATTAGTGCAATATATATGGATTTGGAGGCCTTTCTGCAGAGGTCTAACCTCCTTGCAGATCTC
  CATGCTTTCTGCCAGGCTCACAGCTATGATGTCCTGGTTGCCATGACTATCTTTTTCAACACTCACAAT
  GAGCCAGTGCGGCAGTTGGCTATTTTCTGTCCCCATGTGGCACTCCAAACAACGATCTGTGAAGTCCTG
  GAACGCTCCCACTCTCCACCCCTGAAGCTGACCCCTGCCTCAAGTACCCACCCTAACCTCCATGCCTAT
  CTTCAAGGCAACACCCAGGTCTCTCGAAAGAAACTTCTGCCCCTGCTCCAGGAAGCCCTGTCAGCATAT
  TTTGACTCCATGAAGATCCCTTCAGGACAGCCTGAGACAGCAGATGTGTCCAGGGAGCAAGTGGACAAG
  GAATTGGACAGGGCAAGTAACTCCCTGATTTCTGGCCTGAGTCAAGATGAGGAGGACCCTCCGCTGCCC
  CCGACGCCCATGAACAGCTTGGTGGATGAGTGCCCTCTAGATCAGGGGCTGCCTAAACTCTCTGCTGAG
  GCCGTCTTCGAGAAGTGCAGTCAGATCTCACTGTCACAGTCTACCACAGCCTCCCTGTCCAAGAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG236948
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRLTSMHYTRLANSPSSLSTIISYPRLTTEQMLRKDQKTIYRQGVKVAISAIYMDLEAFLQRSNLLADL
  HAFCQAHSYDVLVAMTIFFNTHNEPVRQLAIFCPHVALQTTICEVLERSHSPPLKLTPASSTHPNLHAY
  LQGNTQVSRKKLLPLLQEALSAYFDSMKIPSGQPETADVSREQVDKELDRASNSLISGLSQDEEDPPLP
  PTPMNSLVDECPLDQGLPKLSAEAVFEKCSQISLSQSTTASLSKK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001303243
• ORF Size: 756 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001303243.2
• RefSeq Size: 2757 bp
• RefSeq ORF: 759 bp
• Locus ID: 58497
• Cytogenetics: 1q21.3
• Protein Pathways: Purine metabolism
• MW: 28.5 kDa
• Gene Summary: This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]