AZI2 (NM_001271650) Human Tagged ORF Clone

CAT#: RG237142

AZI2 (GFP-tagged) - Human 5-azacytidine induced 2 (AZI2), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: AZI2
• Synonyms: AZ2; NAP1; TILP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237142 representing NM_001271650.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAATAAAGACAACTCTGAATCTTTGAAAGTATTGAATGAGCAGCTACAATCTAAAGAAGTAGAACTC
  CTCCAGCTGAGGACAGAGGTGGAAACTCAGCAGGTGATGAGGAATTTAAATCCACCTTCATCAAACTGG
  GAGGTGGAAAAGTTGAGCTGTGACCTGAAGATCCATGGTTTGGAACAAGAGCTGGAACTGATGAGGAAA
  GAATGTAGCGATCTCAAAATAGAACTACAGAAAGCCAAACAAACGGATCCATATCAGGAAGACAATCTG
  AAGAGCAGAGATCTCCAAAAACTAAGCATTTCAAGTGATAATATGCAGCATGCATACTGGGAACTGAAG
  AGAGAAATGTCTAATTTACATCTGGTGACTCAAGTACAAGCTGAACTACTAAGAAAACTGAAAACCTCA
  ACTGCAATCAAGAAAGCCTGTGCCCCTGTAGGATGCAGTGAAGACCTTGGAAGAGACAGCACAAAACTG
  CACTTGATGAATTTTACTGCAACATACACAAGACATCCCCCTCTCTTACCAAATGGCAAAGCTCTTTGT
  CATACCACATCTTCCCCTTTACCAGGAGATGTAAAGGTTTTATCAGAGAAAGCAATCCTCCAATCATGG
  ACAGACAATGAGAGATCCATTCCTAATGATGGTACATGCTTTCAGGAACACAGTTCTTATGGCAGAAAT
  TCTCTGGAAGACAATTCCTGGGTATTTCCAAGTCCTCCTAAATCAAGTGAGACAGCATTTGGGGAAACT
  AAAACTAAAACTTTGCCTTTACCCAACCTTCCACCACTGCATTACTTGGATCAACATAATCAGAACTGC
  CTTTATAAGAAT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237142
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSNWEVEKLSCDLKIHGLEQELELMRK
  ECSDLKIELQKAKQTDPYQEDNLKSRDLQKLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTS
  TAIKKACAPVGCSEDLGRDSTKLHLMNFTATYTRHPPLLPNGKALCHTTSSPLPGDVKVLSEKAILQSW
  TDNERSIPNDGTCFQEHSSYGRNSLEDNSWVFPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQNC
  LYKN
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271650
• ORF Size: 840 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001271650.2
• RefSeq Size: 3474 bp
• RefSeq ORF: 843 bp
• Locus ID: 64343
• Cytogenetics: 3p24.1
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 32.4 kDa
• Gene Summary: AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 [PubMed 14560022]). [supplied by OMIM, Mar 2008]