TID1 (DNAJA3) (NM_001286516) Human Tagged ORF Clone

CAT#: RG237306

DNAJA3 (GFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DNAJA3
• Synonyms: HCA57; hTID-1; TID1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237306 representing NM_001286516.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTGAGCCGCAAGCTGAGCGTCCCCGCCTTTGCGTCTTCCCTGACCTCTTGCGGCCCCCGAGCGCT
  GCTGACATTGAGACCTGGTGTCAGCCTTACAGGAAGATCTTTGGCGAGTTCTCATCCTCTTCATTTGGA
  GATTTCCAGACCGTGTTTGATCAGCCTCAGGAATACTTCATGGAGTTGACATTCAATCAAGCTGCAAAG
  GGGGTCAACAAGGAGTTCACCGTGAACATCATGGACACGTGTGAGCGCTGCAACGGCAAGGGGAACGAG
  CCCGGCACCAAGGTGCAGCATTGCCACTACTGTGGCGGCTCCGGCATGGAAACCATCAACACAGGCCCT
  TTTGTGATGCGTTCCACGTGTAGGAGATGTGGTGGCCGCGGCTCCATCATCATATCGCCCTGTGTGGTC
  TGCAGGGGAGCAGGACAAGCCAAGCAGAAAAAGCGAGTGATGATCCCTGTGCCTGCAGGAGTCGAGGAT
  GGCCAGACCGTGAGGATGCCTGTGGGAAAAAGGGAAATTTTCATTACGTTCAGGGTGCAGAAAAGCCCT
  GTGTTCCGGAGGGACGGCGCAGACATCCACTCCGACCTCTTTATTTCTATAGCTCAGGCTCTTCTTGGG
  GGTACAGCCAGAGCCCAGGGCCTGTACGAGACGATCAACGTGACGATCCCCCCTGGGACTCAGACAGAC
  CAGAAGATTCGGATGGGTGGGAAAGGCATCCCCCGGATTAACAGCTACGGCTACGGAGACCACTACATC
  CACATCAAGATACGAGTTCCAAAGAGGCTAACGAGCCGGCAGCAGAGCCTGATCCTGAGCTACGCCGAG
  GACGAGACAGATGTGGAGGGGACGGTGAACGGCGTCACCCTCACCAGCTCTGGAAAAAGATCCACTGGA
  AAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237306
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAEPQAERPRLCVFPDLLRPPSAADIETWCQPYRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAK
  GVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVV
  CRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLG
  GTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAE
  DETDVEGTVNGVTLTSSGKRSTGN
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286516
• ORF Size: 900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286516.1, NP_001273445.1
• RefSeq Size: 2314 bp
• RefSeq ORF: 903 bp
• Locus ID: 9093
• Cytogenetics: 16p13.3
• MW: 33.5 kDa
• Gene Summary: This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]