HMGCLL1 (NM_001287741) Human Tagged ORF Clone

CAT#: RG237355

HMGCLL1 (GFP-tagged) - Human 3-hydroxymethyl-3-methylglutaryl-CoA lyase-like 1 (HMGCLL1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HMGCLL1
• Synonyms: bA418P12.1; er-cHL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237355 representing NM_001287741.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGAATGTGCCATCCGCGGTGAAGCACTGCCTCAGCTACCAGCAGCTTCTCCGGGAGCATCTCTGG
  ATCGGGGATTCAGTGGCAGGGGCGCTCGACCCCGCGCAGGAAACATCCCAGTTATCTGGACTCCCTGAG
  TTTGTTAAAATAGTAGAAGTTGGGCCTAGGGATGGATTGCAGAATGAAAAGGTTATAGTTCCTACAGAT
  ATAAAAATTGAATTTATCAATCGACTTTCCCAAACTGGCTTGTCTGTAATAGAAGTGACTAGCTTTGTG
  TCTTCCAGATGGGTACCACAGGTTGCTGCTGGAGCTACTGAGATATCAGTTTTTGGAGCTGCATCTGAA
  TCCTTTAGCAAGAAGAATATTAACTGTTCCATTGAAGAAAGTATGGGAAAATTTGAGGAGGTTGTTAAG
  TCTGCAAGACACATGAATATTCCAGCACGAGGGTATGTGTCTTGTGCTCTGGGCTGTCCATATGAAGGA
  AGTATTACACCGCAAAAAGTGACAGAAGTGTCTAAGAGATTGTACGGCATGGGTTGTTATGAGATCTCT
  CTAGGAGACACAATTGGAGTGGGAACTCCAGGAAGTATGAAAAGAATGTTGGAAAGTGTGATGAAAGAA
  ATCCCACCAGGTGCTCTTGCTGTTCACTGTCATGACACATACGGACAAGCCTTAGCAAATATCCTTACG
  GCCCTTCAGATGGGAATTAATGTGGTGGACTCCGCAGTATCCGGATTAGGTGGCTGCCCTTATGCAAAA
  GGTGCTTCTGGGAATGTAGCCACTGAGGATTTGATATATATGCTTAATGGCCTGGGGCTCAATACAGGT
  GTGAATCTATACAAAGTGATGGAAGCTGGTGACTTTATTTGCAAAGCTGTGAATAAAACCACAAACTCT
  AAAGTAGCACAAGCCTCCTTCAATGCT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237355
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGNVPSAVKHCLSYQQLLREHLWIGDSVAGALDPAQETSQLSGLPEFVKIVEVGPRDGLQNEKVIVPTD
  IKIEFINRLSQTGLSVIEVTSFVSSRWVPQVAAGATEISVFGAASESFSKKNINCSIEESMGKFEEVVK
  SARHMNIPARGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGVGTPGSMKRMLESVMKE
  IPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPYAKGASGNVATEDLIYMLNGLGLNTG
  VNLYKVMEAGDFICKAVNKTTNSKVAQASFNA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287741
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001287741.1, NP_001274670.1
• RefSeq Size: 2385 bp
• RefSeq ORF: 927 bp
• Locus ID: 54511
• Cytogenetics: 6p12.1
• MW: 33.2 kDa