HIP55 (DBNL) (NM_001284313) Human Tagged ORF Clone

CAT#: RG237463

DBNL (GFP-tagged) - Human drebrin-like (DBNL), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DBNL
• Synonyms: ABP1; HIP-55; HIP55; SH3P7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237463 representing NM_001284313.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCAGCTTCCTGAAGGGGGCCCATGTGACCATCAACGCACGGGCCGAGGAGGATGTGGAGCCTGAG
  TGCATCATGGAGAAGGTGGCCAAGGCTTCAGGTGCCAACTACAGCTTTCACAAGGAGAGTGGCCGCTTC
  CAGGACGTGGGACCCCAGGCCCCAGTGGGCTCTGTGTACCAGAAGACCAATGCCGTGTCTGAGATTAAA
  AGGGTTGGTAAAGACAGCTTCTGGGCCAAAGCAGAGAAGGAGGAGGAGAACCGTCGGCTGGAGGAAAAG
  CGGCGGGCCGAGGAGGCACAGCGGCAGCTGGAGCAGGAGCGCCGGGAGCGTGAGCTGCGTGAGGCTGCA
  CGCCGGGAGCAGCGCTATCAGGAGCAGGGTGGCGAGGCCAGCCCCCAGAGGACGTGGGAGCAGCAGCAA
  GAAGTGGTTTCAAGGAACCGAAATGAGCAGGAGTCTGCCGTGCACCCGAGGGAGATTTTCAAGCAGAAG
  GAGAGGGCCATGTCCACCACCTCCATCTCCAGTCCTCAGCCTGGCAAGCTGAGGAGCCCCTTCCTGCAG
  AAGCAGCTCACCCAACCAGAGACCCACTTTGGCAGAGAGCCAGCTGCTGCCATCTCAAGGCCCAGGGCA
  GATCTCCCTGCTGAGGAGCCGGCGCCCAGCACTCCTCCATGTCTGGTGCAGGCAGAAGAGGAGGCTGTG
  TATGAGGAACCTCCAGAGCAGGAGACCTTCTACGAGCAGCCCCCACTGGTGCAGCAGCAAGGTGCTGGC
  TCTGAGCACATTGACCACCACATTCAGGGCCAGGGGCTCAGTGGGCAAGGGCTCTGTGCCCGTGCCCTG
  TACGACTACCAGGCAGCCGACGACACAGAGATCTCCTTTGACCCCGAGAACCTCATCACGGGCATCGAG
  GTGATCGACGAAGGCTGGTGGCGTGGCTATGGGCCGGATGGCCATTTTGGCATGTTCCCTGCCAACTAC
  GTGGAGCTCATTGAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237463
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASFLKGAHVTINARAEEDVEPECIMEKVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVSEIK
  RVGKDSFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAARREQRYQEQGGEASPQRTWEQQQ
  EVVSRNRNEQESAVHPREIFKQKERAMSTTSISSPQPGKLRSPFLQKQLTQPETHFGREPAAAISRPRA
  DLPAEEPAPSTPPCLVQAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQGLSGQGLCARAL
  YDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284313
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001284313.2
• RefSeq Size: 2041 bp
• RefSeq ORF: 984 bp
• Locus ID: 28988
• Cytogenetics: 7p13
• MW: 37.6 kDa
• Gene Summary: Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. [UniProtKB/Swiss-Prot Function]