CGK2 (PRKG2) (NM_001282480) Human Tagged ORF Clone

CAT#: RG237586

PRKG2 (GFP-tagged) - Human protein kinase, cGMP-dependent, type II (PRKG2), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PRKG2
• Synonyms: cGK2; cGKII; PKG2; PRKGR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237586 representing NM_001282480.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTAACTGGAAGCTGTCCAAAGCACTCTCTCTGGAAATGATTCAGCTGAAGGAGAAGGTGGCCAGA
  TTTTCCTCATCATCCCCATTCCAGAACCTTGAGATTATTGCAACACTGGGCGTTGGTGGGTTCGGAAGA
  GTTGAGCTTGTTAAAGTAAAAAATGAGAATGTTGCTTTTGCTATGAAGTGTATAAGGAAGAAGCACATA
  GTTGACACCAAGCAGCAGGAGCATGTCTACTCAGAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTC
  ATTGTGAAATTATATCGTACTTTCAAGGACAATAAGTATGTATACATGCTTCTGGAGGCCTGCTTAGGT
  GGGGAGCTCTGGAGTATATTAAGGGACAGAGGCAGCTTTGATGAACCCACCTCCAAATTCTGCGTTGCT
  TGTGTGACAGAAGCATTTGATTACCTGCATCGACTAGGTATTATCTACAGAGACTTGAAACCAGAAAAC
  TTAATTCTAGATGCTGAGGGTTACCTTAAATTGGTTGACTTTGGATTTGCGAAGAAAATAGGGTCTGGA
  CAGAAAACATGGACATTCTGTGGGACTCCAGAATATGTAGCTCCTGAAGTCATTCTCAACAAGGGACAT
  GACTTCAGTGTGGATTTCTGGTCACTGGGAATTCTAGTGTATGAGCTCCTAACGGGCAACCCACCCTTT
  TCTGGGGTTGACCAAATGATGACCTACAATTTGATTCTCAAAGGAATTGAAAAAATGGATTTTCCCAGG
  AAGATAACACGACGACCTGAGGATTTGATTCGGAGGCTTTGCAGGCAAAATCCAACAGAAAGGCTGGGA
  AATCTGAAGAATGGAATAAATGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAGGGACTG
  AAAGCACGGAGCCTTCCATCACCTTTGCAAAGAGAGCTCAAGGGACCCATAGATCACAGCTACTTTGAC
  AAATATCCTCCTGAAAAGGGAATGCCTCCAGATGAGCTATCAGGCTGGGATAAAGACTTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237586
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHI
  VDTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVA
  CVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGH
  DFSVDFWSLGILVYELLTGNPPFSGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLG
  NLKNGINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282480
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282480.1, NP_001269409.1
• RefSeq Size: 3897 bp
• RefSeq ORF: 1029 bp
• Locus ID: 5593
• Cytogenetics: 4q21.21
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction
• MW: 39.9 kDa
• Gene Summary: 'This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical. [provided by RefSeq, May 2018]'