DTX3 (NM_001286246) Human Tagged ORF Clone

CAT#: RG237620

DTX3 (GFP-tagged) - Human deltex 3, E3 ubiquitin ligase (DTX3), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DTX3
• Synonyms: deltex3; RNF154
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237620 representing NM_001286246.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGTTCGTCCTGTCCAGAATGGCAGCCTGTGGAGGCACCTGCAAGAACAAAGTGACTGTGTCCAAG
  CCCGTGTGGGACTTCCTGAGCAAAGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAGCACCGTGTG
  TCCATCCTCATAGATGGCGAGACTTCTGACATCTATGTTCTCCAGCTTTCCCCACAGGGTCCTCCCCCG
  GCCCCTCCAAATGGGCTCTACCTAGCCCGGAAGGCTCTCAAGGGGCTGCTAAAAGAGGCAGAGAAAGAG
  CTGAAGAAAGCTCAGAGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGGGGAGCACCCT
  GAGATGCACCGCGCAGGCCCACCCCCTCTCCGAGCAGCCCCACTTCTGCCCCCAGGAGCTCGGGGGCTC
  CCCCCTCCTCCTCCCCCCCTGCCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCAGAAGAGCAGGAG
  AGCACCTGCCCCATCTGTCTGGGGGAGATCCAGAATGCCAAGACATTGGAGAAGTGCCGGCATTCATTC
  TGCGAGGGCTGCATCACCCGGGCTCTGCAGGTGAAAAAGGCCTGCCCCATGTGCGGCCGCTTCTATGGG
  CAGCTGGTGGGCAACCAGCCCCAGAATGGGCGGATGCTGGTCTCTAAGGACGCCACCCTCCTACTGCCC
  AGCTATGAGAAGTACGGCACCATTGTCATCCAGTACGTCTTCCCGCCCGGTGTCCAGGGGGCTGAACAC
  CCAAACCCAGGAGTTCGGTATCCTGGCACCACACGGGTGGCCTACCTCCCGGACTGCCCTGAGGGCAAC
  AAGGTGCTGACCCTGTTCCGCAAGGCGTTTGACCAGCGTCTCACCTTCACTATCGGCACGTCCATGACC
  ACAGGGAGACCGAATGTCATCACCTGGAACGACATCCACCACAAGACCAGCTGCACAGGGGGACCCCAG
  CTGTTTGGGTACCCAGACCCCACCTACCTGACCCGGGTGCAAGAGGAGCTGAGAGCGAAGGGTATCACA
  GATGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237620
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSFVLSRMAACGGTCKNKVTVSKPVWDFLSKETPARLARLREEHRVSILIDGETSDIYVLQLSPQGPPP
  APPNGLYLARKALKGLLKEAEKELKKAQRQGELMGCLALGGGGEHPEMHRAGPPPLRAAPLLPPGARGL
  PPPPPPLPPPLPPRLREEAEEQESTCPICLGEIQNAKTLEKCRHSFCEGCITRALQVKKACPMCGRFYG
  QLVGNQPQNGRMLVSKDATLLLPSYEKYGTIVIQYVFPPGVQGAEHPNPGVRYPGTTRVAYLPDCPEGN
  KVLTLFRKAFDQRLTFTIGTSMTTGRPNVITWNDIHHKTSCTGGPQLFGYPDPTYLTRVQEELRAKGIT
  DD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286246
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286246.2
• RefSeq Size: 2081 bp
• RefSeq ORF: 1044 bp
• Locus ID: 196403
• Cytogenetics: 12q13.3
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 38.4 kDa
• Gene Summary: DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]